Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NSS81_RS05185 Genome accession   NZ_CP151943
Coordinates   974048..975097 (-) Length   349 a.a.
NCBI ID   WP_342432475.1    Uniprot ID   -
Organism   Neobacillus sp. FSL H8-0543     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 969048..980097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS81_RS05160 (NSS81_05160) - 969684..970490 (-) 807 WP_342432470.1 PilN domain-containing protein -
  NSS81_RS05165 (NSS81_05165) pilM 970487..971476 (-) 990 WP_342432471.1 pilus assembly protein PilM -
  NSS81_RS05170 (NSS81_05170) - 971493..972248 (-) 756 WP_342432472.1 prepilin peptidase -
  NSS81_RS05175 (NSS81_05175) - 972248..972709 (-) 462 WP_342432473.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NSS81_RS05180 (NSS81_05180) - 972850..974055 (-) 1206 WP_342432474.1 type II secretion system F family protein -
  NSS81_RS05185 (NSS81_05185) pilT 974048..975097 (-) 1050 WP_342432475.1 type IV pilus twitching motility protein PilT Machinery gene
  NSS81_RS05190 (NSS81_05190) - 975110..976771 (-) 1662 WP_342432476.1 ATPase, T2SS/T4P/T4SS family -
  NSS81_RS05195 (NSS81_05195) - 976795..978093 (-) 1299 WP_342432477.1 hypothetical protein -
  NSS81_RS05200 (NSS81_05200) - 978118..978894 (-) 777 WP_342432478.1 UbiA family prenyltransferase -
  NSS81_RS05205 (NSS81_05205) - 979161..979625 (+) 465 WP_342432479.1 type II secretion system protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38922.06 Da        Isoelectric Point: 9.0130

>NTDB_id=981522 NSS81_RS05185 WP_342432475.1 974048..975097(-) (pilT) [Neobacillus sp. FSL H8-0543]
MKEKIDKILRAALEFKASDIHLTVGVPPVFRINGDIKRYGKEILLPDDTEEIARLTVPEDMYTVFKEKGELDYSYGIPGL
SRFRVNAYHQRRSVSLALRVVASKTPTIEDLDLPEIVAKLVEKPQGLILVTGPTGSGKSTTLASMIDYVNRTMRKHIVTL
EDPIEYLHKHGNSIIDQREVGFDTKSYANGLKGALRQDPDIILVGEMRDLETIGIAITAAETGHLVLGTLHTSSAPSTIN
RIIDVFPPAQQPQIRVQLASVLVGVISQRLFPTVDKKGRKAATEILVNNPAIANLIRNEKIHQIQSTMQTSRAQGMHTLE
MSLRELLDRNLIQKEAAQKYLQEKMMMDG

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=981522 NSS81_RS05185 WP_342432475.1 974048..975097(-) (pilT) [Neobacillus sp. FSL H8-0543]
ATGAAAGAAAAAATCGATAAAATACTGAGGGCAGCACTTGAATTTAAAGCGTCCGATATTCATTTAACAGTTGGTGTCCC
ACCTGTATTTCGAATCAATGGGGATATTAAGCGGTATGGAAAAGAAATTTTGCTTCCTGATGATACCGAGGAAATTGCAA
GGCTGACAGTTCCAGAGGACATGTATACTGTTTTTAAGGAAAAAGGAGAGCTGGATTATTCTTACGGTATTCCTGGGCTA
TCGCGCTTCCGTGTTAACGCCTATCATCAACGGAGGAGTGTTTCCCTAGCCCTAAGGGTGGTTGCTTCAAAAACACCGAC
AATTGAAGATTTAGATTTACCAGAAATAGTTGCGAAACTAGTTGAAAAGCCTCAGGGTCTTATCCTAGTGACGGGACCGA
CAGGAAGCGGAAAATCAACTACCCTTGCATCAATGATTGATTATGTAAACAGAACGATGCGCAAGCATATCGTTACCCTT
GAGGATCCAATTGAGTATCTCCACAAACACGGAAATTCGATTATTGACCAACGCGAAGTCGGGTTTGATACCAAATCATA
TGCAAATGGCTTAAAGGGTGCCTTACGACAAGATCCTGACATTATTCTAGTTGGGGAAATGCGTGACTTAGAAACGATTG
GGATTGCGATTACGGCAGCAGAAACCGGACACCTTGTGCTAGGAACACTTCATACCTCAAGTGCCCCGTCAACCATTAAC
CGGATAATCGATGTATTTCCACCGGCACAGCAGCCGCAAATCCGTGTTCAGCTTGCATCTGTATTGGTGGGCGTCATTTC
GCAGCGCTTGTTTCCTACTGTTGACAAAAAGGGACGTAAGGCTGCAACTGAAATCTTAGTAAACAATCCGGCGATTGCAA
ACTTAATCCGAAATGAAAAAATCCACCAAATTCAAAGTACGATGCAAACGTCTCGAGCCCAGGGGATGCATACACTTGAA
ATGAGTTTAAGAGAGTTACTAGATCGAAACCTGATTCAAAAGGAAGCGGCCCAGAAATATTTACAAGAGAAGATGATGAT
GGATGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.049

93.983

0.499
  pilT Neisseria gonorrhoeae MS11

46.991

100

0.47
  pilT Neisseria meningitidis 8013

46.705

100

0.467
  pilT Pseudomonas stutzeri DSM 10701

47.788

97.135

0.464
  pilT Acinetobacter baylyi ADP1

47.337

96.848

0.458
  pilT Vibrio cholerae strain A1552

47.041

96.848

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.041

96.848

0.456
  pilT Legionella pneumophila strain ERS1305867

46.866

95.989

0.45
  pilT Legionella pneumophila strain Lp02

46.866

95.989

0.45
  pilT Acinetobacter nosocomialis M2

46.707

95.702

0.447
  pilT Acinetobacter baumannii D1279779

46.707

95.702

0.447
  pilT Acinetobacter baumannii strain A118

46.707

95.702

0.447
  pilT Pseudomonas aeruginosa PAK

46.567

95.989

0.447
  pilU Pseudomonas stutzeri DSM 10701

41.916

95.702

0.401
  pilU Acinetobacter baylyi ADP1

40.525

98.281

0.398
  pilU Vibrio cholerae strain A1552

39.521

95.702

0.378

Multiple sequence alignment