Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAFF32_RS07230 Genome accession   NZ_CP151802
Coordinates   1567205..1568317 (-) Length   370 a.a.
NCBI ID   WP_216960540.1    Uniprot ID   A0AAP2H9G8
Organism   Lysobacter sp. FW306-1B-D06B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1562205..1573317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFF32_RS07195 (AAFF32_07195) coq7 1562232..1562876 (-) 645 WP_342316942.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  AAFF32_RS07200 (AAFF32_07200) - 1562886..1564082 (-) 1197 WP_342316943.1 (Fe-S)-binding protein -
  AAFF32_RS07205 (AAFF32_07205) rplM 1564254..1564682 (+) 429 WP_216960552.1 50S ribosomal protein L13 -
  AAFF32_RS07210 (AAFF32_07210) rpsI 1564709..1565101 (+) 393 WP_216960549.1 30S ribosomal protein S9 -
  AAFF32_RS07220 (AAFF32_07220) - 1565426..1566415 (+) 990 WP_342316944.1 fumarylacetoacetate hydrolase family protein -
  AAFF32_RS07225 (AAFF32_07225) maiA 1566482..1567150 (+) 669 WP_216960543.1 maleylacetoacetate isomerase -
  AAFF32_RS07230 (AAFF32_07230) pilU 1567205..1568317 (-) 1113 WP_216960540.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAFF32_RS07235 (AAFF32_07235) - 1568493..1568795 (+) 303 WP_342316945.1 DUF4398 domain-containing protein -
  AAFF32_RS07240 (AAFF32_07240) - 1568792..1569676 (+) 885 WP_342316946.1 hypothetical protein -
  AAFF32_RS07245 (AAFF32_07245) - 1570053..1570271 (+) 219 WP_216960532.1 YdcH family protein -
  AAFF32_RS07250 (AAFF32_07250) - 1570518..1571894 (+) 1377 WP_216960530.1 pyridoxal-phosphate dependent enzyme -
  AAFF32_RS07255 (AAFF32_07255) - 1571894..1572838 (+) 945 WP_342316947.1 2OG-Fe(II) oxygenase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41054.41 Da        Isoelectric Point: 5.9389

>NTDB_id=980928 AAFF32_RS07230 WP_216960540.1 1567205..1568317(-) (pilU) [Lysobacter sp. FW306-1B-D06B]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPLFERDLELNMALALPEAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDYRNSNISGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLRAVVSQRLVIGTDGRRLPAAEVLINTPHIRDLMRRGQVHEIKQAMEETLEEGMESFDQCL
FRLHKEGKIDMEEALKAADSREGLALKFRLSEGSSGEHDPYADIYDAATQ

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=980928 AAFF32_RS07230 WP_216960540.1 1567205..1568317(-) (pilU) [Lysobacter sp. FW306-1B-D06B]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTTTTGACCACCGGCGCGCCGGTTTA
CATCAAGGTCGAAGGCAAGCTCTATCCCCTGGGCAATACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCATACTCGT
TGATGGACGAGGGGCAAGTTCCGCTGTTCGAGCGTGACCTCGAACTGAACATGGCGCTCGCCCTGCCCGAAGCCGGCCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCCATCCGCAGCGTGATCCCGTCGATCGA
GGAACTGCAACTGCCGCAGGTGCTGAAGGACATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTCGGCTCTACCGGTT
CGGGCAAGTCGACCACGCTCGCGTCGATGATCGACTACCGCAACAGCAACATCTCGGGCCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGAAGTCGATCGTGAACCAGCGCGAAGTCGGCCTGGACACGCACGCCTTCCACAA
CGCGCTGAAGAATGCGATGCGCGAGGCGCCGGACGTCATCCTGATCGGCGAAATCCTCGACGCCACTACGATGGAAGCGG
CGATCGCGTTTGCCGAAACCGGCCACTTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACGCTGGAGCGCATC
CTCAACTTCTTCCCGGAAGCGGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTCAGTCAGCG
ACTGGTGATCGGCACCGACGGGCGCCGCCTGCCGGCCGCGGAAGTGCTGATCAACACGCCGCACATCCGCGACCTCATGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAGGAAACGCTGGAGGAAGGCATGGAATCCTTCGACCAGTGCCTG
TTCCGCCTGCACAAGGAAGGCAAGATCGACATGGAAGAGGCCCTGAAGGCCGCCGACTCGCGCGAAGGCCTGGCGCTCAA
GTTCCGCCTCTCCGAAGGCTCCAGCGGCGAGCACGACCCGTACGCGGACATCTACGACGCCGCGACGCAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

97.027

0.5
  pilU Acinetobacter baylyi ADP1

48.305

95.676

0.462
  pilU Vibrio cholerae strain A1552

48.023

95.676

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.972

96.757

0.435
  pilT Acinetobacter baumannii strain A118

41.279

92.973

0.384
  pilT Acinetobacter nosocomialis M2

41.279

92.973

0.384
  pilT Acinetobacter baumannii D1279779

41.279

92.973

0.384
  pilT Pseudomonas aeruginosa PAK

41.716

91.351

0.381
  pilT Acinetobacter baylyi ADP1

40.988

92.973

0.381
  pilT Pseudomonas stutzeri DSM 10701

40.407

92.973

0.376
  pilT Legionella pneumophila strain Lp02

40.533

91.351

0.37
  pilT Legionella pneumophila strain ERS1305867

40.533

91.351

0.37
  pilT Neisseria gonorrhoeae MS11

39.193

93.784

0.368
  pilT Neisseria meningitidis 8013

39.193

93.784

0.368
  pilT Vibrio cholerae strain A1552

40.24

90

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.24

90

0.362

Multiple sequence alignment