Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAEY27_RS04000 Genome accession   NZ_CP151800
Coordinates   848510..849490 (+) Length   326 a.a.
NCBI ID   WP_342323629.1    Uniprot ID   -
Organism   Kosakonia sp. BYX6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 843510..854490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEY27_RS03970 (AAEY27_03970) wzz(fepE) 843987..845093 (+) 1107 WP_342323623.1 LPS O-antigen length regulator Wzz(fepE) -
  AAEY27_RS03975 (AAEY27_03975) hemW 845185..846321 (-) 1137 WP_342323624.1 radical SAM family heme chaperone HemW -
  AAEY27_RS03980 (AAEY27_03980) - 846314..846907 (-) 594 WP_342323625.1 XTP/dITP diphosphatase -
  AAEY27_RS03985 (AAEY27_03985) yggU 846916..847206 (-) 291 WP_342323626.1 DUF167 family protein YggU -
  AAEY27_RS03990 (AAEY27_03990) - 847203..847769 (-) 567 WP_342323627.1 YggT family protein -
  AAEY27_RS03995 (AAEY27_03995) - 847788..848492 (-) 705 WP_342323628.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAEY27_RS04000 (AAEY27_04000) pilT 848510..849490 (+) 981 WP_342323629.1 type IV pilus twitching motility protein PilT Machinery gene
  AAEY27_RS04005 (AAEY27_04005) - 849487..851745 (-) 2259 WP_342323630.1 FAD-dependent oxidoreductase -
  AAEY27_RS04010 (AAEY27_04010) - 851812..852504 (+) 693 WP_342323631.1 IclR family transcriptional regulator -
  AAEY27_RS04015 (AAEY27_04015) ruvX 852661..853077 (-) 417 WP_342323632.1 Holliday junction resolvase RuvX -
  AAEY27_RS04020 (AAEY27_04020) - 853077..853640 (-) 564 WP_342323633.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35451.38 Da        Isoelectric Point: 6.1711

>NTDB_id=980906 AAEY27_RS04000 WP_342323629.1 848510..849490(+) (pilT) [Kosakonia sp. BYX6]
MDIEEIVALSVKHNVSDLHLCSGCPPRWRQQGRLEPAPFDLPDIEALIDAQLNALQQRQWREQGQVDFALSTASGRRLRA
GAFSRYGGSSLALRLLPQSCPLLEEIDTPAALPTLLGRENGLILVTGATGSGKSTTLAAMVQHLNQAYDAHILTLEDPIE
FVHTSARSLIQQREVGQHCASFADGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDT
FPAQEKDPVRSQLAGSLQAVLAQKLEADKQGGRVALFELLINTPAVANLIREGKTHQLPGVMQTGQQVGMQTFAQSHQQR
VLQGWL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=980906 AAEY27_RS04000 WP_342323629.1 848510..849490(+) (pilT) [Kosakonia sp. BYX6]
ATGGATATAGAAGAAATAGTGGCGCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCAGTGGTTGCCCGCCGCG
CTGGCGACAACAGGGACGGCTGGAGCCTGCACCTTTTGATCTGCCTGATATTGAGGCGCTGATCGACGCGCAGCTCAATG
CGCTACAGCAGCGCCAGTGGCGTGAACAGGGGCAGGTCGATTTCGCGTTGAGCACCGCTAGCGGCAGGCGGCTGCGCGCC
GGGGCTTTTTCTCGTTATGGCGGGTCTTCGCTGGCGCTGCGTTTGCTGCCGCAAAGCTGTCCGTTACTGGAAGAGATCGA
TACCCCCGCCGCATTGCCGACGCTGCTTGGGCGGGAAAATGGGTTGATTCTGGTGACCGGCGCGACCGGCAGCGGCAAAT
CCACCACGCTGGCGGCGATGGTTCAGCACCTTAATCAGGCTTATGACGCCCATATTCTGACGCTGGAAGACCCGATCGAG
TTTGTGCACACCAGCGCGCGCAGCCTGATCCAGCAGCGGGAAGTCGGCCAGCACTGCGCGTCTTTTGCCGATGGGCTGCG
CGCGGCGCTGCGTGAAGATCCCGATGTGATTCTGTTGGGGGAACTTCGCGACAGCGAGACTATTCGCCTGGCGCTGACCG
CCGCCGAAACCGGACATCTGGTGATGGCGACCTTGCATACGCGCGGTGCCGCGCAAGCCGTCGAGCGACTGGTGGATACC
TTTCCGGCGCAAGAAAAAGACCCGGTGCGCAGCCAGTTAGCGGGGAGTTTGCAGGCGGTGCTGGCGCAAAAGCTGGAAGC
GGACAAGCAGGGTGGGCGTGTGGCGCTGTTCGAATTGCTGATCAATACGCCTGCGGTGGCGAATTTAATCCGTGAAGGCA
AGACGCATCAACTGCCCGGCGTGATGCAAACCGGGCAGCAAGTGGGGATGCAGACTTTTGCGCAAAGCCATCAACAGCGG
GTGCTACAGGGCTGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.456

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.456

100

0.509
  pilT Neisseria meningitidis 8013

50

100

0.509
  pilT Neisseria gonorrhoeae MS11

49.699

100

0.506
  pilT Pseudomonas aeruginosa PAK

47.416

100

0.479
  pilT Acinetobacter baylyi ADP1

47.416

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

49.051

96.933

0.475
  pilT Acinetobacter baumannii strain A118

46.809

100

0.472
  pilT Acinetobacter baumannii D1279779

46.809

100

0.472
  pilT Acinetobacter nosocomialis M2

46.809

100

0.472
  pilT Legionella pneumophila strain ERS1305867

48.113

97.546

0.469
  pilT Legionella pneumophila strain Lp02

48.113

97.546

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.912

97.546

0.448
  pilU Vibrio cholerae strain A1552

40.923

99.693

0.408
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Acinetobacter baylyi ADP1

38.318

98.466

0.377

Multiple sequence alignment