Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WN949_RS03845 Genome accession   NZ_CP151088
Coordinates   792397..793377 (+) Length   326 a.a.
NCBI ID   WP_341516968.1    Uniprot ID   -
Organism   Citrobacter gillenii strain DW61     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 787397..798377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WN949_RS03815 ansB 787902..788948 (+) 1047 WP_096755739.1 L-asparaginase 2 -
  WN949_RS03820 hemW 789073..790209 (-) 1137 WP_117342645.1 radical SAM family heme chaperone HemW -
  WN949_RS03825 - 790202..790795 (-) 594 WP_182260673.1 XTP/dITP diphosphatase -
  WN949_RS03830 yggU 790803..791093 (-) 291 WP_042310947.1 DUF167 family protein YggU -
  WN949_RS03835 - 791090..791656 (-) 567 WP_096755742.1 YggT family protein -
  WN949_RS03840 - 791675..792379 (-) 705 WP_096755743.1 YggS family pyridoxal phosphate-dependent enzyme -
  WN949_RS03845 pilT 792397..793377 (+) 981 WP_341516968.1 type IV pilus twitching motility protein PilT Machinery gene
  WN949_RS03850 ruvX 793387..793803 (-) 417 WP_096755745.1 Holliday junction resolvase RuvX -
  WN949_RS03855 - 793803..794366 (-) 564 WP_096755746.1 YqgE/AlgH family protein -
  WN949_RS03860 gshB 794542..795489 (-) 948 WP_117342642.1 glutathione synthase -
  WN949_RS03865 rsmE 795502..796233 (-) 732 WP_096755748.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WN949_RS03870 endA 796308..797015 (-) 708 WP_341516969.1 deoxyribonuclease I -
  WN949_RS03875 - 797253..798128 (+) 876 WP_341516970.1 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35886.08 Da        Isoelectric Point: 6.5677

>NTDB_id=974795 WN949_RS03845 WP_341516968.1 792397..793377(+) (pilT) [Citrobacter gillenii strain DW61]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRVEIAPFTAPDVEKLLMLWLSEQQQVQWREKGQVDFAIALADSRRLRA
SAFTHQQGTSLALRLLPFDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVDSLNQHVDGHILTLEDPIE
YRYTSQRCLIQQREIGVHCLSFAAGLRAALREDPDVILLGELRDSETIRLALTAVETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEADRQGGRVALFELLVNTSAVSHLIREGKTHQLPGVIQTGQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=974795 WN949_RS03845 WP_341516968.1 792397..793377(+) (pilT) [Citrobacter gillenii strain DW61]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGGTAGAAATTGCCCCGTTTACCGCGCCTGACGTGGAGAAGCTGCTGATGTTATGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAAGGGTCAGGTTGATTTTGCTATCGCACTGGCGGATTCCCGGCGTTTACGGGCC
AGCGCATTTACGCATCAGCAGGGAACGTCACTGGCGCTGAGACTGCTGCCGTTTGATTGCCCGTGCCTGGATGATCTCCA
GACGCCCGCTGCGTTGCCAGAACTGCTGCACAGCGAAAACGGGTTGATTCTGGTGACCGGGGCGACGGGTAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTCTCTCAATCAGCACGTTGACGGGCATATTCTGACCCTGGAAGACCCGATTGAA
TACCGCTATACCAGCCAGCGTTGTCTGATCCAACAGCGTGAGATTGGCGTGCACTGCCTTTCTTTTGCTGCCGGCCTGCG
GGCGGCATTGCGTGAAGATCCTGACGTGATTTTACTGGGGGAACTGCGCGACAGCGAGACGATTCGTCTGGCACTGACGG
CGGTGGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGTGCGGCGCAGGCGGTGGAGCGGCTGGTGGATACG
TTCCCTGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGTAGTTTGCGGGCGGTGCTCGCACAAAAACTGGAAGC
CGACAGGCAGGGTGGGCGGGTGGCGTTATTTGAACTGCTGGTCAATACGTCGGCTGTGAGTCATCTGATCCGTGAAGGGA
AAACCCACCAGCTACCTGGCGTGATACAAACCGGGCAACAGGCGGGTATGCAAACATTTGCCCAGAGCCTGCAGCAGCGT
CAGGCGCAAGGCAGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Pseudomonas stutzeri DSM 10701

44.343

100

0.445
  pilT Pseudomonas aeruginosa PAK

44.037

100

0.442
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.082

97.546

0.42
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368

Multiple sequence alignment