Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AABC73_RS02295 Genome accession   NZ_CP151076
Coordinates   486059..487093 (+) Length   344 a.a.
NCBI ID   WP_020291795.1    Uniprot ID   A0A085V219
Organism   Pseudomonas sp. G.S.17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481059..492093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABC73_RS02265 (AABC73_02265) - 481421..481858 (-) 438 WP_341522278.1 DUF4426 domain-containing protein -
  AABC73_RS02270 (AABC73_02270) metW 481884..482504 (-) 621 WP_065831733.1 methionine biosynthesis protein MetW -
  AABC73_RS02275 (AABC73_02275) - 482512..483651 (-) 1140 WP_341522279.1 homoserine O-acetyltransferase -
  AABC73_RS02280 (AABC73_02280) - 483855..484445 (-) 591 WP_341522280.1 YggT family protein -
  AABC73_RS02285 (AABC73_02285) proC 484474..485292 (-) 819 WP_341522281.1 pyrroline-5-carboxylate reductase -
  AABC73_RS02290 (AABC73_02290) - 485317..486003 (-) 687 WP_341522282.1 YggS family pyridoxal phosphate-dependent enzyme -
  AABC73_RS02295 (AABC73_02295) pilT 486059..487093 (+) 1035 WP_020291795.1 type IV pilus twitching motility protein PilT Machinery gene
  AABC73_RS02300 (AABC73_02300) - 487212..487889 (-) 678 WP_341522283.1 C40 family peptidase -
  AABC73_RS02305 (AABC73_02305) - 488140..488559 (+) 420 WP_341522284.1 TM2 domain-containing protein -
  AABC73_RS02310 (AABC73_02310) - 488770..490119 (+) 1350 WP_341522285.1 hypothetical protein -
  AABC73_RS02315 (AABC73_02315) - 490238..491509 (-) 1272 WP_341522286.1 dihydroorotase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37950.75 Da        Isoelectric Point: 6.6632

>NTDB_id=974683 AABC73_RS02295 WP_020291795.1 486059..487093(+) (pilT) [Pseudomonas sp. G.S.17]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALSAKEVQELIYDIMNDKQRQNFEELLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMVDYLNCNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAQEKSMIRSMLSESLHAVVSQTLLKKVGGGRIAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCM
KDLVTRGLVTRDSAREKAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=974683 AABC73_RS02295 WP_020291795.1 486059..487093(+) (pilT) [Pseudomonas sp. G.S.17]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAGCAAGGCGCATCGGACTTGCATCTCTCTGCTGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGATGTGCGGCGGATCAACCTGCCAGCCCTGAGCGCCAAAGAGGTTCAGGAGCTGATCTACGACA
TCATGAACGACAAGCAACGGCAGAACTTCGAAGAGCTGCTGGAAACCGACTTTTCCTTCGAAGTGCCGGGCGTTGCACGC
TTTCGGGTCAACGCGTTCAACCAGAACCGTGGCGCGGGCGCGGTGTTTCGGACCATTCCTTCGAAGATCCTGAGCATGGA
AGATCTCGGGATGGGAGAAGTGTTTCGCAAGATTACCGACGTGCCGCGCGGCCTGGTCCTGGTCACCGGACCGACCGGTT
CCGGCAAGTCGACGACCCTGGCAGCGATGGTCGATTACCTGAACTGCAACAAACATCACCACATCCTGACCATCGAAGAC
CCGATCGAATTCGTTCACGAGTCCAAGAAGTGCCTGGTCAACCAGCGCGAAGTGCACCGCGATACCCTCGGCTTTTCGGA
AGCCTTGCGCTCGGCGCTGCGTGAAGACCCGGACGTGATTCTGGTGGGCGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACGGCGGCGGAAACCGGTCACCTGGTGTTTGGCACGCTGCACACCACGTCCGCAGCGAAGACCATCGACCGGATC
GTCGACGTGTTCCCGGCGCAGGAAAAATCCATGATTCGTTCGATGTTGTCCGAGTCGCTGCATGCCGTGGTGTCGCAAAC
CCTGCTGAAGAAGGTCGGCGGCGGGCGGATTGCTGCGCACGAAATCATGATGGGTACGCCAGCGATCAGGAACCTGATTC
GCGAAGACAAGGTCGCACAGATGTACTCAGCGATTCAGACCGGAGGCTCGCTGGGTATGCAGACGCTGGATATGTGCATG
AAGGATCTGGTCACCCGCGGCCTGGTTACCCGCGACAGCGCGCGGGAAAAGGCCAAGGTGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A085V219

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

89.244

100

0.892
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Legionella pneumophila strain Lp02

72.781

98.256

0.715
  pilT Legionella pneumophila strain ERS1305867

72.781

98.256

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria meningitidis 8013

66.765

98.837

0.66
  pilT Neisseria gonorrhoeae MS11

66.471

98.837

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment