Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VUK78_RS13160 Genome accession   NZ_CP150467
Coordinates   545412..546452 (+) Length   346 a.a.
NCBI ID   WP_005425810.1    Uniprot ID   A0A9X3MNX0
Organism   Vibrio harveyi strain NH-LM1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540412..551452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUK78_RS13125 (VUK78_13125) hemW 540431..541603 (-) 1173 WP_029789656.1 radical SAM family heme chaperone HemW -
  VUK78_RS13130 (VUK78_13130) - 541622..542224 (-) 603 WP_005436525.1 XTP/dITP diphosphatase -
  VUK78_RS13135 (VUK78_13135) - 542330..542761 (-) 432 WP_005425804.1 DUF4426 domain-containing protein -
  VUK78_RS13140 (VUK78_13140) yggU 542822..543112 (-) 291 WP_005425805.1 DUF167 family protein YggU -
  VUK78_RS13145 (VUK78_13145) - 543112..543669 (-) 558 WP_005425806.1 YggT family protein -
  VUK78_RS13150 (VUK78_13150) proC 543723..544541 (-) 819 WP_050937496.1 pyrroline-5-carboxylate reductase -
  VUK78_RS13155 (VUK78_13155) - 544673..545383 (-) 711 WP_340700191.1 YggS family pyridoxal phosphate-dependent enzyme -
  VUK78_RS13160 (VUK78_13160) pilT 545412..546452 (+) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  VUK78_RS13165 (VUK78_13165) pilU 546484..547590 (+) 1107 WP_005449736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VUK78_RS13170 (VUK78_13170) ruvX 547650..548075 (-) 426 WP_005449738.1 Holliday junction resolvase RuvX -
  VUK78_RS13175 (VUK78_13175) - 548125..548688 (-) 564 WP_005449740.1 YqgE/AlgH family protein -
  VUK78_RS13180 (VUK78_13180) gshB 548818..549768 (-) 951 WP_340700192.1 glutathione synthase -
  VUK78_RS13185 (VUK78_13185) rsmE 549783..550514 (-) 732 WP_005449742.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VUK78_RS13190 (VUK78_13190) endA 550661..551356 (-) 696 WP_005449743.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38634.12 Da        Isoelectric Point: 6.5043

>NTDB_id=970409 VUK78_RS13160 WP_005425810.1 545412..546452(+) (pilT) [Vibrio harveyi strain NH-LM1]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=970409 VUK78_RS13160 WP_005425810.1 545412..546452(+) (pilT) [Vibrio harveyi strain NH-LM1]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTAAGAAAGCTGGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTTTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCTGTGTTCCGTACCATCCCAAGTAGCATTCCAACACTGGA
AGAGCTAGAAGCCCCAGAGATCTTCAGAAAGATTGCCAATGCTGAAAAAGGCCTGGTATTGGTTACCGGTCCAACGGGTT
CTGGTAAATCGACTACCTTGGCAGCCATTGTTGACTACATTAACCGTAATCACAATAAGCACGTCCTAACAATTGAAGAT
CCGATTGAATTTGTTCACACCAACAATAAGTGTCTGATCAACCAACGTGAAGTACACCGTGATACACACAGCTTCCAAAA
CGCACTGCGCAGCGCATTACGTGAAGACCCGGATGTGATTCTAGTTGGTGAGATGCGTGATAAAGAAACCATCAGTTTGG
CGCTGACGGCTGCTGAAACGGGTCACCTTGTTTTCGGTACGCTGCACACTAGCTCAGCAGCGAAAACCATCGACCGTATT
ATCGACGTATTCCCAGGTAGTGACAAAGACATGGTGCGTTCGATGCTGTCGGAGTCGCTGCGTTCGGTTATTGCACAAAA
ACTGTTAAAACGTAATGGTGGTGGTCGTATTGCTTGTCATGAAATCATGATGGCGACACCTGCGATCCGTAACCTTATTC
GTGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAGACCGGTGCGGCGCATGGCATGCAGACCATGGAGCAGAATGCT
CGTCAATTGATGGCGCAAGGCATGGTATCGCGTGAAGAAGTGGATAAGAAAGTTGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.377

99.711

0.861
  pilT Vibrio cholerae strain A1552

86.377

99.711

0.861
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Pseudomonas aeruginosa PAK

68.824

98.266

0.676
  pilT Pseudomonas stutzeri DSM 10701

69.027

97.977

0.676
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

64.118

98.266

0.63
  pilT Neisseria gonorrhoeae MS11

63.824

98.266

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.857

100

0.503
  pilU Vibrio cholerae strain A1552

41.617

96.532

0.402
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.532

0.39

Multiple sequence alignment