Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VUK78_RS13165 Genome accession   NZ_CP150467
Coordinates   546484..547590 (+) Length   368 a.a.
NCBI ID   WP_005449736.1    Uniprot ID   A0A9X3RZ62
Organism   Vibrio harveyi strain NH-LM1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541484..552590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUK78_RS13130 (VUK78_13130) - 541622..542224 (-) 603 WP_005436525.1 XTP/dITP diphosphatase -
  VUK78_RS13135 (VUK78_13135) - 542330..542761 (-) 432 WP_005425804.1 DUF4426 domain-containing protein -
  VUK78_RS13140 (VUK78_13140) yggU 542822..543112 (-) 291 WP_005425805.1 DUF167 family protein YggU -
  VUK78_RS13145 (VUK78_13145) - 543112..543669 (-) 558 WP_005425806.1 YggT family protein -
  VUK78_RS13150 (VUK78_13150) proC 543723..544541 (-) 819 WP_050937496.1 pyrroline-5-carboxylate reductase -
  VUK78_RS13155 (VUK78_13155) - 544673..545383 (-) 711 WP_340700191.1 YggS family pyridoxal phosphate-dependent enzyme -
  VUK78_RS13160 (VUK78_13160) pilT 545412..546452 (+) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  VUK78_RS13165 (VUK78_13165) pilU 546484..547590 (+) 1107 WP_005449736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VUK78_RS13170 (VUK78_13170) ruvX 547650..548075 (-) 426 WP_005449738.1 Holliday junction resolvase RuvX -
  VUK78_RS13175 (VUK78_13175) - 548125..548688 (-) 564 WP_005449740.1 YqgE/AlgH family protein -
  VUK78_RS13180 (VUK78_13180) gshB 548818..549768 (-) 951 WP_340700192.1 glutathione synthase -
  VUK78_RS13185 (VUK78_13185) rsmE 549783..550514 (-) 732 WP_005449742.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VUK78_RS13190 (VUK78_13190) endA 550661..551356 (-) 696 WP_005449743.1 deoxyribonuclease I -
  VUK78_RS13195 (VUK78_13195) - 551508..552005 (-) 498 WP_009697583.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41263.37 Da        Isoelectric Point: 6.7729

>NTDB_id=970410 VUK78_RS13165 WP_005449736.1 546484..547590(+) (pilU) [Vibrio harveyi strain NH-LM1]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLSENDVALLLDSAMDPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKATMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=970410 VUK78_RS13165 WP_005449736.1 546484..547590(+) (pilU) [Vibrio harveyi strain NH-LM1]
ATGGATCTGAATAAATTTCTCGAAGGCATGCTGGCGTTAAAAGCGTCGGATCTATACATCACTGTCGGTGCCCCGATCTT
GTTTCGTGTGGATGGCGAATTGCGTCCGCAAGGCGATAAGCTGAGCGAAAATGACGTAGCCTTGTTGCTTGATAGTGCGA
TGGACCCGGATCGACGACAGGAGTTTCGCAAAAGCCGCGAGTCGAACTTTGCCATTGTCCGTGATTGCGGTCGCTTCCGT
GTCAGTGCCTTCTTCCAACGTGAATTACCGGGAGCCGTCATTCGTCGTATTGAAACCAATATTCCAACGTTTGAGCAGCT
TAAGTTGCCTTTGGTGCTGCAAGATCTTGCAATTGCCAAGCGTGGTTTGGTGCTGGTGGTTGGTGCGACAGGCTCTGGTA
AGTCGACCACCATGGCTGCGATGACGGGTTATCGCAACAACAACAAGACTGGTCATATTCTTACGGTAGAAGACCCAATT
GAATTTGTGCATGAGCATAAACGTTGTATTGTGACTCAGCGTGAAGTGGGCTTGGATACTGACAGCTATGAAGTGGCGTT
GAAGAACTCGTTGCGTCAGGCACCAGACATGATTTTGATTGGTGAGATCCGAAGCCGAGAAACGATGGAATACGCAATGA
CCTTTGCTGAAACTGGTCACTTGTGCATGGCTACATTGCACGCCAACAACGCAAACCAAGCACTTGAACGTATTTTGCAC
TTGGTGCCGAAAGACCAGAAAGATCAGTTCTTGTTTGATCTGTCGATGAACTTAAAAGGCGTAGTAGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAATAGCCCGCGTGTATCCGATCTGATCCGTC
GAGGCGATCTTCATGAGTTGAAAGCAACCATGGCTAGATCCAATGAATTTGGGATGTTGACCTTCGACCAATCGCTCTAC
AAGTTAGTGATGCAAGGAAAGATCAGTGAAGAAGATGCATTGCACAGTGCGGACTCTGCCAACGATCTGCGTTTGATGTT
GAAGACGCAACGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

59.143

95.109

0.563
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.57

93.478

0.389
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.643

92.935

0.378
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37

Multiple sequence alignment