Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKY27_RS01295 Genome accession   NZ_CP150239
Coordinates   258484..259527 (+) Length   347 a.a.
NCBI ID   WP_339197050.1    Uniprot ID   -
Organism   Solibacillus sp. FSL R5-0449     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 253484..264527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY27_RS01275 (MKY27_01275) - 254060..254527 (-) 468 WP_339197042.1 type II secretion system protein -
  MKY27_RS01280 (MKY27_01280) - 254529..254975 (-) 447 WP_339197044.1 type II secretion system protein -
  MKY27_RS01285 (MKY27_01285) - 255388..256791 (+) 1404 WP_339197045.1 VanW family protein -
  MKY27_RS01290 (MKY27_01290) - 256807..258471 (+) 1665 WP_339197048.1 GspE/PulE family protein -
  MKY27_RS01295 (MKY27_01295) pilT 258484..259527 (+) 1044 WP_339197050.1 type IV pilus twitching motility protein PilT Machinery gene
  MKY27_RS01300 (MKY27_01300) - 259524..260732 (+) 1209 WP_339197052.1 type II secretion system F family protein -
  MKY27_RS01305 (MKY27_01305) - 260827..261243 (+) 417 WP_339197055.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKY27_RS01310 (MKY27_01310) pilM 261367..262344 (+) 978 WP_339197058.1 pilus assembly protein PilM -
  MKY27_RS01315 (MKY27_01315) - 262341..262931 (+) 591 WP_339197061.1 PilN domain-containing protein -
  MKY27_RS01320 (MKY27_01320) - 262931..263641 (+) 711 WP_339197064.1 potassium transporter -
  MKY27_RS01325 (MKY27_01325) - 263674..264429 (+) 756 WP_339197066.1 prepilin peptidase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38356.77 Da        Isoelectric Point: 6.1248

>NTDB_id=967294 MKY27_RS01295 WP_339197050.1 258484..259527(+) (pilT) [Solibacillus sp. FSL R5-0449]
MTLNIQDLLQHAYDENASDLHVTTGIPPVYRINGQLKQYGDVIVTAEMIEQMVKDILPSYKAAEFDEKGETDFNYSLKDF
CRFRVNAYHQRNVGAIAARLISSEIPTIDSLNMPNVLYDLAEKPQGLILVTGPTGSGKSTTLAAMIDYINETKSKHIITL
EDPIEYLHSHKKSVVNQREIGVDTGSFANGLRASLRQDPDIILVGEMRDLETISTAITAAETGHLVFGTLHTSSAPTTID
RIIDVFPPHQQGQIRIQLANVLQGIISQRLFIRKDKAGRVAATEILVSIPAVTNLIRNEKIHQIPSVMQTNRALGMHTLE
TSIQALVSSGKVSLEEVRPYLNVGDYS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=967294 MKY27_RS01295 WP_339197050.1 258484..259527(+) (pilT) [Solibacillus sp. FSL R5-0449]
ATGACATTGAACATACAAGATTTACTGCAGCATGCGTATGATGAAAATGCTTCCGATTTGCATGTCACTACTGGCATCCC
TCCTGTTTACCGAATAAATGGTCAGCTGAAACAATATGGCGATGTAATTGTAACGGCGGAAATGATTGAGCAAATGGTAA
AAGATATTTTACCATCTTACAAAGCAGCTGAGTTTGATGAAAAGGGTGAAACTGATTTCAACTATTCATTGAAAGACTTT
TGCCGCTTCCGTGTAAATGCATATCATCAGCGAAATGTTGGTGCAATCGCAGCTCGTCTTATCTCGAGTGAAATTCCGAC
AATTGACTCACTGAATATGCCGAATGTGCTGTACGATTTAGCAGAAAAACCTCAAGGACTAATTTTAGTAACTGGTCCAA
CTGGTTCAGGTAAGTCAACTACACTAGCGGCAATGATTGATTATATTAATGAAACAAAATCAAAACATATTATTACACTG
GAAGACCCGATTGAATATTTACATTCGCATAAAAAATCGGTAGTGAATCAGCGTGAAATCGGAGTCGATACCGGTTCATT
TGCAAATGGTCTGCGTGCATCGCTCCGTCAAGATCCGGATATTATTCTAGTGGGGGAAATGCGAGATTTAGAGACGATTT
CTACAGCGATTACGGCAGCAGAAACAGGTCACTTAGTATTCGGCACACTTCATACGTCAAGTGCACCGACAACCATTGAC
CGTATTATCGATGTATTCCCGCCACATCAGCAAGGGCAGATTCGAATTCAATTAGCTAACGTATTGCAAGGAATTATTTC
ACAGCGATTATTCATTCGTAAAGATAAAGCAGGCCGGGTTGCAGCAACAGAAATTCTCGTAAGTATTCCGGCTGTAACAA
ATTTGATACGAAATGAAAAAATTCATCAGATTCCAAGTGTGATGCAGACAAATCGAGCGTTAGGGATGCATACATTGGAG
ACATCCATACAAGCGCTTGTTTCTTCCGGTAAAGTTTCATTGGAGGAAGTACGGCCATATTTGAATGTGGGTGATTACAG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.308

93.66

0.49
  pilT Pseudomonas aeruginosa PAK

49.555

97.118

0.481
  pilT Neisseria meningitidis 8013

49.405

96.83

0.478
  pilT Acinetobacter nosocomialis M2

49.258

97.118

0.478
  pilT Neisseria gonorrhoeae MS11

49.107

96.83

0.476
  pilT Acinetobacter baumannii D1279779

48.961

97.118

0.476
  pilT Acinetobacter baumannii strain A118

48.961

97.118

0.476
  pilT Acinetobacter baylyi ADP1

48.665

97.118

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.512

96.83

0.47
  pilT Vibrio cholerae strain A1552

48.512

96.83

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.368

97.118

0.47
  pilT Legionella pneumophila strain Lp02

48.338

95.389

0.461
  pilT Legionella pneumophila strain ERS1305867

48.338

95.389

0.461
  pilU Pseudomonas stutzeri DSM 10701

39.82

96.254

0.383
  pilU Vibrio cholerae strain A1552

40.566

91.643

0.372
  pilU Acinetobacter baylyi ADP1

38.554

95.677

0.369

Multiple sequence alignment