Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKY07_RS01460 Genome accession   NZ_CP150208
Coordinates   294090..295136 (+) Length   348 a.a.
NCBI ID   WP_339263575.1    Uniprot ID   -
Organism   Solibacillus sp. FSL W7-1472     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 289090..300136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY07_RS01440 (MKY07_01440) - 289642..290157 (-) 516 WP_339263571.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKY07_RS01445 (MKY07_01445) - 290150..290563 (-) 414 WP_339263572.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKY07_RS01450 (MKY07_01450) - 290975..292393 (+) 1419 WP_339263573.1 VanW family protein -
  MKY07_RS01455 (MKY07_01455) - 292411..294075 (+) 1665 WP_339263574.1 ATPase, T2SS/T4P/T4SS family -
  MKY07_RS01460 (MKY07_01460) pilT 294090..295136 (+) 1047 WP_339263575.1 type IV pilus twitching motility protein PilT Machinery gene
  MKY07_RS01465 (MKY07_01465) - 295133..296341 (+) 1209 WP_339263577.1 type II secretion system F family protein -
  MKY07_RS01470 (MKY07_01470) - 296435..296914 (+) 480 WP_339263579.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKY07_RS01475 (MKY07_01475) pilM 297043..298020 (+) 978 WP_339263580.1 pilus assembly protein PilM -
  MKY07_RS01480 (MKY07_01480) - 298017..298610 (+) 594 WP_339263581.1 PilN domain-containing protein -
  MKY07_RS01485 (MKY07_01485) - 298607..299380 (+) 774 WP_339263582.1 potassium transporter -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38523.14 Da        Isoelectric Point: 6.3437

>NTDB_id=966388 MKY07_RS01460 WP_339263575.1 294090..295136(+) (pilT) [Solibacillus sp. FSL W7-1472]
MATLYIQDLLQRAYDENASDLHVTIGIPPVYRVNGQLKHYGDVIVTPEMVEQMIQTILPTYKAAEFEEKGETDFNYSLEG
LCRFRVNAYHQRNAGAIAARLISSKIPTIESLNMPKVLYDLAEKPQGLILVTGPTGSGKSTTLAAMIDYINETKAKHIIT
LEDPIEYLHSHKKSVVNQREVGVDTDTFANGLRASLRQDPDIILVGEMRDLDTISTAITAAETGHLVFATLHTSSAPTTI
DRIIDVFPPYQQGQIRIQLANVLQGIISQRLFIRKDKAGRVAATEILVSVPAVTNLIRNEKIHQIPSIMQTSRALGMHTL
ENSIQALISSGKISLEEARPYMNVGDYS

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=966388 MKY07_RS01460 WP_339263575.1 294090..295136(+) (pilT) [Solibacillus sp. FSL W7-1472]
ATGGCGACATTATACATTCAAGATTTACTGCAGCGTGCGTATGACGAAAACGCTTCTGATTTACATGTAACGATCGGGAT
TCCTCCTGTATACCGCGTAAACGGTCAATTGAAGCATTACGGAGATGTCATCGTTACACCGGAAATGGTCGAACAGATGA
TTCAGACGATTTTACCGACATATAAAGCAGCGGAATTCGAGGAAAAAGGCGAAACCGATTTCAACTATTCACTGGAAGGA
CTTTGCCGATTCCGTGTCAATGCTTATCACCAGCGAAATGCCGGGGCAATTGCCGCTCGTCTGATTTCGAGCAAAATCCC
GACAATTGAGTCATTGAATATGCCAAAAGTGCTGTACGATCTGGCAGAAAAACCTCAGGGACTTATTTTAGTTACAGGTC
CAACGGGGTCAGGGAAATCGACAACACTTGCTGCAATGATCGACTATATAAATGAAACAAAAGCAAAGCATATCATTACG
TTAGAAGACCCGATTGAGTATTTACATTCGCACAAAAAATCGGTCGTCAATCAGCGTGAAGTCGGTGTCGATACAGATAC
GTTCGCAAATGGTCTTCGCGCATCACTGCGTCAGGACCCGGATATCATTCTGGTAGGGGAAATGCGAGATTTAGATACGA
TTTCAACAGCGATTACCGCTGCGGAAACCGGTCACTTAGTATTTGCCACACTGCATACATCAAGTGCACCGACAACGATT
GACCGGATTATCGATGTGTTCCCGCCTTATCAGCAGGGGCAAATCCGGATTCAGTTGGCGAATGTATTGCAGGGCATCAT
TTCACAACGGCTGTTTATCCGCAAAGATAAAGCAGGGCGTGTAGCGGCAACAGAAATTTTAGTGAGTGTGCCTGCTGTGA
CGAACTTAATCCGAAATGAAAAAATTCACCAGATTCCAAGTATTATGCAAACAAGCAGGGCTTTGGGGATGCACACATTG
GAAAACTCGATCCAGGCACTTATTTCTTCCGGGAAAATTTCATTGGAGGAAGCTCGTCCATATATGAACGTAGGTGATTA
CAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.94

96.264

0.5
  pilT Pseudomonas stutzeri DSM 10701

51.045

96.264

0.491
  pilT Acinetobacter baylyi ADP1

51.045

96.264

0.491
  pilT Acinetobacter nosocomialis M2

50.448

96.264

0.486
  pilT Acinetobacter baumannii strain A118

50.149

96.264

0.483
  pilT Acinetobacter baumannii D1279779

50.149

96.264

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.926

93.103

0.474
  pilT Neisseria meningitidis 8013

48.81

96.552

0.471
  pilT Vibrio cholerae strain A1552

49.102

95.977

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.102

95.977

0.471
  pilT Neisseria gonorrhoeae MS11

48.512

96.552

0.468
  pilT Legionella pneumophila strain ERS1305867

49.24

94.54

0.466
  pilT Legionella pneumophila strain Lp02

49.24

94.54

0.466
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.264

0.399
  pilU Vibrio cholerae strain A1552

40.994

92.529

0.379
  pilU Acinetobacter baylyi ADP1

39.521

95.977

0.379

Multiple sequence alignment