Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKZ14_RS05765 Genome accession   NZ_CP150202
Coordinates   1220807..1221853 (+) Length   348 a.a.
NCBI ID   WP_016991992.1    Uniprot ID   A0AAE8QS02
Organism   Lysinibacillus sp. FSL P2-0066     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1215807..1226853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKZ14_RS05745 (MKZ14_05745) - 1216338..1216784 (-) 447 WP_333006157.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKZ14_RS05750 (MKZ14_05750) - 1216765..1217247 (-) 483 WP_333006156.1 type II secretion system protein -
  MKZ14_RS05755 (MKZ14_05755) - 1217777..1219108 (+) 1332 WP_333006155.1 G5 domain-containing protein -
  MKZ14_RS05760 (MKZ14_05760) - 1219132..1220796 (+) 1665 WP_258859611.1 GspE/PulE family protein -
  MKZ14_RS05765 (MKZ14_05765) pilT 1220807..1221853 (+) 1047 WP_016991992.1 type IV pilus twitching motility protein PilT Machinery gene
  MKZ14_RS05770 (MKZ14_05770) - 1221850..1223055 (+) 1206 WP_036080216.1 type II secretion system F family protein -
  MKZ14_RS05775 (MKZ14_05775) - 1223179..1223400 (+) 222 WP_333006154.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKZ14_RS05780 (MKZ14_05780) - 1223363..1223656 (+) 294 WP_238542680.1 hypothetical protein -
  MKZ14_RS05785 (MKZ14_05785) - 1223713..1224159 (+) 447 WP_333006153.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKZ14_RS05790 (MKZ14_05790) pilM 1224272..1225246 (+) 975 WP_016991996.1 pilus assembly protein PilM -
  MKZ14_RS05795 (MKZ14_05795) - 1225246..1225839 (+) 594 WP_258859482.1 hypothetical protein -
  MKZ14_RS05800 (MKZ14_05800) - 1225836..1226528 (+) 693 WP_333006152.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38914.79 Da        Isoelectric Point: 7.0543

>NTDB_id=966216 MKZ14_RS05765 WP_016991992.1 1220807..1221853(+) (pilT) [Lysinibacillus sp. FSL P2-0066]
MTAYSIVELLTRAFEEKASDLHLTKGIPPVYRIHGQLEHYGTVPLETEDLKEMVKVILPVHKQQEFEDKGETDFNFSLPG
KCRFRVNAYHQRNEVTIAARLIEAKIPSIEALNMPQVLYKLAEKPQGLILVTGPTGSGKSTTLAAMIDHINETRSKHIIT
LEDPIEYLHTHKKSVINQREIGVDTMSFANGLRASLRQDPDIILVGEMRDYETISTAITAAETGHLVMATLHTSSAPTTI
DRIIDVFPPHQQSQIRVQLANVLQGIVSQRLFVRKDVKGRIAATEILVQAPAISNLIRNEKIHQIPSVMQTSKALGMHTL
EYSMQHLITTGKISLEDARPYMNAGDYS

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=966216 MKZ14_RS05765 WP_016991992.1 1220807..1221853(+) (pilT) [Lysinibacillus sp. FSL P2-0066]
ATGACAGCATATTCAATCGTCGAATTATTAACACGTGCATTTGAAGAGAAAGCATCTGATCTTCATTTAACAAAAGGTAT
TCCACCGGTATATCGTATCCATGGGCAACTGGAGCATTATGGCACAGTCCCACTTGAAACAGAGGATTTAAAGGAAATGG
TAAAGGTCATTCTTCCTGTCCATAAACAACAGGAATTTGAGGACAAGGGAGAAACGGACTTTAACTTTTCATTGCCTGGG
AAATGTCGATTCCGTGTCAATGCTTACCATCAACGAAATGAAGTAACGATTGCAGCTCGTTTAATTGAGGCTAAAATTCC
TTCTATTGAAGCGCTCAATATGCCACAGGTGTTATATAAGCTTGCAGAAAAACCACAAGGCTTAATATTAGTGACAGGCC
CAACAGGCTCAGGGAAATCAACTACCTTAGCAGCAATGATAGATCATATCAATGAAACAAGATCTAAGCATATTATTACG
TTAGAGGATCCAATTGAATATTTGCATACACATAAGAAGTCTGTCATCAACCAACGTGAGATTGGTGTCGATACAATGAG
CTTTGCCAATGGCTTACGAGCATCATTACGACAGGACCCAGATATTATTTTGGTTGGGGAGATGCGAGATTATGAAACCA
TTTCAACGGCTATTACAGCTGCTGAAACAGGGCATTTAGTCATGGCTACACTTCATACAAGTAGTGCACCAACGACCATC
GACCGTATTATCGATGTATTTCCACCGCATCAGCAGAGTCAAATTCGTGTGCAGCTTGCGAATGTACTACAAGGTATTGT
TTCTCAGCGTTTATTTGTACGTAAGGATGTAAAAGGGCGTATTGCTGCGACAGAAATTTTAGTACAGGCACCTGCAATTT
CAAATTTAATTCGTAACGAAAAAATTCATCAAATTCCAAGTGTGATGCAGACAAGTAAAGCTTTGGGTATGCACACATTG
GAATATTCGATGCAGCACCTAATTACGACAGGTAAGATTTCACTAGAGGATGCAAGACCATATATGAACGCTGGTGATTA
TAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.083

96.552

0.503
  pilT Neisseria meningitidis 8013

50

95.977

0.48
  pilT Neisseria gonorrhoeae MS11

49.701

95.977

0.477
  pilT Acinetobacter baumannii D1279779

49.401

95.977

0.474
  pilT Acinetobacter nosocomialis M2

49.401

95.977

0.474
  pilT Acinetobacter baumannii strain A118

49.401

95.977

0.474
  pilT Pseudomonas aeruginosa PAK

49.102

95.977

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.503

95.977

0.466
  pilT Vibrio cholerae strain A1552

48.503

95.977

0.466
  pilT Legionella pneumophila strain Lp02

49.24

94.54

0.466
  pilT Legionella pneumophila strain ERS1305867

49.24

94.54

0.466
  pilT Pseudomonas stutzeri DSM 10701

48.204

95.977

0.463
  pilT Acinetobacter baylyi ADP1

47.904

95.977

0.46
  pilU Pseudomonas stutzeri DSM 10701

42.598

95.115

0.405
  pilU Vibrio cholerae strain A1552

42.236

92.529

0.391
  pilU Acinetobacter baylyi ADP1

40.06

95.402

0.382

Multiple sequence alignment