Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NSQ78_RS02795 Genome accession   NZ_CP150199
Coordinates   611647..612774 (-) Length   375 a.a.
NCBI ID   WP_049416904.1    Uniprot ID   A0AAP7J565
Organism   Stenotrophomonas sp. FSL W7-1447     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 606647..617774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ78_RS02775 (NSQ78_02775) - 607075..608064 (+) 990 WP_057494091.1 fumarylacetoacetate hydrolase family protein -
  NSQ78_RS02780 (NSQ78_02780) maiA 608079..608747 (+) 669 WP_064237583.1 maleylacetoacetate isomerase -
  NSQ78_RS02785 (NSQ78_02785) - 608792..610252 (-) 1461 WP_064237584.1 UdgX family uracil-DNA binding protein -
  NSQ78_RS02790 (NSQ78_02790) - 610233..611516 (-) 1284 WP_064237585.1 putative DNA modification/repair radical SAM protein -
  NSQ78_RS02795 (NSQ78_02795) pilU 611647..612774 (-) 1128 WP_049416904.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NSQ78_RS02800 (NSQ78_02800) - 612896..613261 (+) 366 WP_019660954.1 DUF4398 domain-containing protein -
  NSQ78_RS02805 (NSQ78_02805) - 613258..614142 (+) 885 WP_080473096.1 OmpA family protein -
  NSQ78_RS02810 (NSQ78_02810) - 614467..614703 (+) 237 WP_005407964.1 YdcH family protein -
  NSQ78_RS02815 (NSQ78_02815) - 615005..616375 (+) 1371 WP_049416902.1 pyridoxal-phosphate dependent enzyme -
  NSQ78_RS02820 (NSQ78_02820) - 616499..617671 (+) 1173 WP_064237586.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41398.70 Da        Isoelectric Point: 6.3741

>NTDB_id=966048 NSQ78_RS02795 WP_049416904.1 611647..612774(-) (pilU) [Stenotrophomonas sp. FSL W7-1447]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKAGTIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=966048 NSQ78_RS02795 WP_049416904.1 611647..612774(-) (pilU) [Stenotrophomonas sp. FSL W7-1447]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTTTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCGCTGGGCAACACCGGCCTGCCGCCAGGCATGGTGAAGAAGATTGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGAGAGCTCGAGCTCAACATGGCCATCGCCCTGCCCGACGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTGAACCTTCCACAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAAACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCAGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGCCGACGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAAATCAAGGCCGCGATGGAAGCCTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCACCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCACGACCCGTACGCGGACTTCTCGGCAGGCGCGCCCAGCATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.471

90.667

0.376
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363

Multiple sequence alignment