Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NSU02_RS00580 Genome accession   NZ_CP150165
Coordinates   110491..111543 (-) Length   350 a.a.
NCBI ID   WP_063387121.1    Uniprot ID   A0A165Y9I5
Organism   Aeribacillus sp. FSL W8-0870     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 105491..116543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSU02_RS00555 (NSU02_00555) - 106039..106962 (-) 924 WP_339193792.1 hypothetical protein -
  NSU02_RS00560 (NSU02_00560) - 106955..107557 (-) 603 WP_339193794.1 PilN domain-containing protein -
  NSU02_RS00565 (NSU02_00565) pilM 107558..108553 (-) 996 WP_339193795.1 pilus assembly protein PilM -
  NSU02_RS00570 (NSU02_00570) - 108670..109083 (-) 414 WP_339193796.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NSU02_RS00575 (NSU02_00575) - 109299..110504 (-) 1206 WP_327873329.1 type II secretion system F family protein -
  NSU02_RS00580 (NSU02_00580) pilT 110491..111543 (-) 1053 WP_063387121.1 type IV pilus twitching motility protein PilT Machinery gene
  NSU02_RS00585 (NSU02_00585) - 111556..113211 (-) 1656 WP_339193797.1 GspE/PulE family protein -
  NSU02_RS00590 (NSU02_00590) - 113284..114675 (-) 1392 WP_339193798.1 G5 domain-containing protein -
  NSU02_RS00595 (NSU02_00595) - 115351..115818 (+) 468 WP_339193800.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NSU02_RS00600 (NSU02_00600) - 115811..116314 (+) 504 WP_339193802.1 hypothetical protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39294.88 Da        Isoelectric Point: 6.9859

>NTDB_id=964449 NSU02_RS00580 WP_063387121.1 110491..111543(-) (pilT) [Aeribacillus sp. FSL W8-0870]
MSYNISDLLKKAFTDHASDLHLIVDSPPVFRINGELKPYGDHSLSKNDTERMAKELIPETMWDTFEQQGELDFNYELEGI
SRFRLNAFRQRNHISVAIRIIPTKIPTIAELKIPSIVEKLMEKPQGLILVTGPTGSGKSTTLAAMIDYINRNYSKHIITL
EDPIEYIHSHKRSIINQREIGSDTKSFANGLRASLRQDPDIILVGEMRDLETISTAITAAETGHLVLATLHTSSAAQTID
RIIDVFPPHQQRQIRLQVANVLQGIISQRLFPLKDGSGRTVATEILINNPSISNLIRNEKIHQIHNVLQTNRAQGMHTLE
MSVQSLLSSGKISYESAMPYINKGDENGYL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=964449 NSU02_RS00580 WP_063387121.1 110491..111543(-) (pilT) [Aeribacillus sp. FSL W8-0870]
ATGTCATACAATATATCAGATTTACTTAAAAAAGCTTTTACGGATCATGCTTCAGACCTCCATTTAATTGTCGACTCTCC
TCCGGTTTTTCGCATAAACGGCGAGCTAAAGCCTTACGGAGACCATTCATTGTCTAAAAACGACACGGAAAGAATGGCAA
AAGAGCTTATTCCGGAGACGATGTGGGATACGTTCGAGCAGCAGGGCGAGCTTGATTTTAACTATGAATTAGAGGGAATT
TCACGGTTTCGATTAAACGCTTTTCGCCAACGCAATCATATAAGTGTCGCGATCCGAATTATACCGACGAAGATCCCAAC
GATCGCAGAGCTTAAAATACCTTCCATTGTCGAGAAATTGATGGAAAAGCCCCAAGGGTTAATTCTCGTCACGGGACCAA
CCGGTTCAGGTAAGTCAACGACATTGGCAGCGATGATCGATTATATTAATCGAAACTATTCCAAACATATTATTACATTA
GAAGATCCAATCGAATATATTCATTCCCATAAGCGATCCATTATCAATCAGCGGGAAATTGGTTCAGATACAAAAAGCTT
TGCAAACGGACTGCGCGCATCCTTAAGGCAAGACCCGGATATTATTTTAGTGGGGGAAATGCGGGATCTTGAGACGATAT
CGACAGCTATTACTGCTGCTGAAACAGGCCATTTAGTGCTGGCCACATTGCACACAAGCAGTGCAGCTCAGACGATCGAC
CGGATCATAGATGTGTTCCCGCCCCATCAGCAAAGGCAAATCCGCCTTCAAGTAGCAAACGTTTTGCAAGGAATCATTTC
ACAGCGCCTTTTCCCGCTAAAGGACGGATCCGGGCGAACGGTTGCAACGGAGATTTTAATTAATAATCCTTCCATTTCAA
ACTTGATTCGCAATGAAAAAATTCATCAAATCCACAATGTTTTGCAAACGAATAGAGCTCAAGGAATGCATACATTGGAA
ATGTCCGTTCAAAGCTTATTAAGCTCAGGAAAAATTTCTTACGAAAGCGCCATGCCATATATCAATAAAGGTGATGAAAA
TGGCTATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A165Y9I5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.448

95.714

0.483
  pilT Neisseria meningitidis 8013

49.249

95.143

0.469
  pilT Neisseria gonorrhoeae MS11

48.949

95.143

0.466
  pilT Acinetobacter baylyi ADP1

47.633

96.571

0.46
  pilT Acinetobacter nosocomialis M2

47.904

95.429

0.457
  pilT Vibrio cholerae strain A1552

47.337

96.571

0.457
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.337

96.571

0.457
  pilT Acinetobacter baumannii D1279779

47.605

95.429

0.454
  pilT Acinetobacter baumannii strain A118

47.605

95.429

0.454
  pilT Pseudomonas aeruginosa PAK

46.707

95.429

0.446
  pilT Pseudomonas stutzeri DSM 10701

46.407

95.429

0.443
  pilT Legionella pneumophila strain Lp02

44.311

95.429

0.423
  pilT Legionella pneumophila strain ERS1305867

44.311

95.429

0.423
  pilU Pseudomonas stutzeri DSM 10701

41.003

96.857

0.397
  pilU Vibrio cholerae strain A1552

41.018

95.429

0.391
  pilU Acinetobacter baylyi ADP1

39.359

98

0.386

Multiple sequence alignment