Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WMO33_RS14775 Genome accession   NZ_CP150117
Coordinates   3281923..3282960 (-) Length   345 a.a.
NCBI ID   WP_014502535.1    Uniprot ID   G7TJ39
Organism   Xanthomonas oryzae pv. oryzicola strain MD-P1-1B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3276923..3287960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WMO33_RS14745 (WMO33_14705) - 3277633..3278067 (+) 435 WP_014502540.1 hypothetical protein -
  WMO33_RS14750 (WMO33_14710) - 3278064..3278636 (+) 573 WP_014502539.1 DNA-3-methyladenine glycosylase I -
  WMO33_RS14755 (WMO33_14715) - 3278673..3279506 (+) 834 WP_014502538.1 DUF72 domain-containing protein -
  WMO33_RS14765 (WMO33_14725) - 3279733..3280407 (-) 675 WP_024711780.1 YitT family protein -
  WMO33_RS14770 (WMO33_14730) pilU 3280679..3281809 (-) 1131 WP_014502536.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WMO33_RS14775 (WMO33_14735) pilT 3281923..3282960 (-) 1038 WP_014502535.1 type IV pilus twitching motility protein PilT Machinery gene
  WMO33_RS14780 (WMO33_14740) - 3283326..3284015 (+) 690 WP_047339652.1 YggS family pyridoxal phosphate-dependent enzyme -
  WMO33_RS14785 (WMO33_14745) proC 3284059..3284919 (+) 861 WP_014502533.1 pyrroline-5-carboxylate reductase -
  WMO33_RS14790 (WMO33_14750) - 3285469..3285894 (+) 426 WP_014502532.1 HU family DNA-binding protein -
  WMO33_RS14795 (WMO33_14755) - 3286001..3286642 (+) 642 WP_047339651.1 M23 family metallopeptidase -
  WMO33_RS14800 (WMO33_14760) - 3286699..3287040 (+) 342 WP_024712273.1 DUF3861 family protein -
  WMO33_RS14805 (WMO33_14765) - 3287098..3287640 (+) 543 WP_047339650.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38657.32 Da        Isoelectric Point: 6.6652

>NTDB_id=962878 WMO33_RS14775 WP_014502535.1 3281923..3282960(-) (pilT) [Xanthomonas oryzae pv. oryzicola strain MD-P1-1B]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALLYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=962878 WMO33_RS14775 WP_014502535.1 3281923..3282960(-) (pilT) [Xanthomonas oryzae pv. oryzicola strain MD-P1-1B]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCGCTTGACCATAAGCAGGTGCACGCGCTGTTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCGTTTGAAATTCCGTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCTTCTGAAGTGCTGACGCTGGA
AGATCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCTTGATCCTGGTGACCGGCCCGACTGGTT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAATCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGTCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCAGTGATTTCGCAGGC
GCTGCTGAAGAAAGTCGGCGGCGGGCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTCGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATTCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G7TJ39

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Vibrio cholerae strain A1552

40.896

97.101

0.397
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397