Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WKR89_RS15255 Genome accession   NZ_CP150073
Coordinates   3254436..3255569 (+) Length   377 a.a.
NCBI ID   WP_011407601.1    Uniprot ID   A0A0K0GQ53
Organism   Xanthomonas oryzae pv. oryzae strain C2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3249436..3260569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKR89_RS15230 (WKR89_15190) - 3249611..3250804 (-) 1194 WP_011257665.1 cystathionine gamma-synthase -
  WKR89_RS15235 (WKR89_15195) - 3250895..3252265 (-) 1371 WP_011407603.1 pyridoxal-phosphate dependent enzyme -
  WKR89_RS15240 (WKR89_15200) - 3252507..3252725 (-) 219 WP_003484103.1 YdcH family protein -
  WKR89_RS15245 (WKR89_15205) - 3253067..3253957 (-) 891 WP_011257662.1 membrane protein -
  WKR89_RS15250 (WKR89_15210) - 3253954..3254337 (-) 384 WP_011407602.1 DUF4398 domain-containing protein -
  WKR89_RS15255 (WKR89_15215) pilU 3254436..3255569 (+) 1134 WP_011407601.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WKR89_RS15260 (WKR89_15220) maiA 3255908..3256534 (-) 627 WP_042464484.1 maleylacetoacetate isomerase -
  WKR89_RS15265 (WKR89_15225) - 3256578..3257636 (-) 1059 WP_178383140.1 fumarylacetoacetate hydrolase family protein -
  WKR89_RS15270 (WKR89_15230) - 3257947..3259329 (+) 1383 WP_011407600.1 DEAD/DEAH box helicase -
  WKR89_RS15275 (WKR89_15235) queD 3259742..3260095 (+) 354 WP_027703598.1 6-carboxytetrahydropterin synthase QueD -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41796.09 Da        Isoelectric Point: 6.0564

>NTDB_id=962747 WKR89_RS15255 WP_011407601.1 3254436..3255569(+) (pilU) [Xanthomonas oryzae pv. oryzae strain C2]
MDIGYFLKLMTEKNASDMFLTTGAPVHIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNIAIALPDAGR
FRVNAFKQRGEVGMVIRAIRSRIPSIEELHLPQVLKDIIMAPRGLVLVVGSTGSGKSTSLAAMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHNNVLMNLALNLRAVISQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEDGSGEHDPYADYETPAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=962747 WKR89_RS15255 WP_011407601.1 3254436..3255569(+) (pilU) [Xanthomonas oryzae pv. oryzae strain C2]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGCA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGATTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAACTCGAACTCAACATTGCCATCGCCCTGCCCGACGCCGGCCGC
TTCCGCGTGAACGCGTTCAAACAGCGCGGTGAGGTAGGCATGGTGATCCGTGCCATTCGCAGCCGGATCCCCAGCATCGA
AGAGCTCCACCTGCCACAGGTGCTCAAGGACATCATCATGGCCCCGCGCGGGCTGGTGCTGGTGGTGGGCTCGACCGGCT
CGGGCAAGTCGACCTCGCTTGCCGCGATGATCGACCACCGCAACAGCACAACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGATACCCATGCTTTCCAGAG
CGCGCTGAAGAATGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAGACCATGGAGGCAG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGTATC
CTCAATTTCTTCCCGGAAAGCGCGCACAATAACGTGCTGATGAACCTGGCGCTCAACCTGCGCGCGGTCATCTCCCAGCG
TCTGGTCAAGGGCCTGGACGGCCGTCGCCGCCCGGCCACCGAAGTACTGCTCAATACGCCGATGATCCGCGACCTGCTAC
GCCGCGGCCAGGTGCACGAGGTCAAGCAGGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGCATGGTGAAGTCGGGCGAAATCGAGCAGGAAGAAGCGCTGCGTGCCGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGTCTCTCCGAAGACGGCTCCGGCGAGCACGACCCGTACGCGGATTACGAAACGCCGGCTGCGCCGAAGATCAGCC
ACGGGTTTATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K0GQ53

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.646

95.225

0.501
  pilU Vibrio cholerae strain A1552

50.556

95.491

0.483
  pilU Acinetobacter baylyi ADP1

49.58

94.695

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.989

99.204

0.456
  pilT Pseudomonas aeruginosa PAK

43.491

89.655

0.39
  pilT Acinetobacter baumannii strain A118

42.604

89.655

0.382
  pilT Acinetobacter nosocomialis M2

42.604

89.655

0.382
  pilT Acinetobacter baumannii D1279779

42.604

89.655

0.382
  pilT Pseudomonas stutzeri DSM 10701

42.308

89.655

0.379
  pilT Acinetobacter baylyi ADP1

41.471

90.186

0.374
  pilT Legionella pneumophila strain Lp02

41.716

89.655

0.374
  pilT Legionella pneumophila strain ERS1305867

41.716

89.655

0.374
  pilT Neisseria meningitidis 8013

40.346

92.042

0.371
  pilT Neisseria gonorrhoeae MS11

40.346

92.042

0.371
  pilT Vibrio cholerae strain A1552

42.042

88.329

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.042

88.329

0.371