Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WKR89_RS12025 Genome accession   NZ_CP150073
Coordinates   2580870..2581907 (-) Length   345 a.a.
NCBI ID   WP_011258220.1    Uniprot ID   A0A0K0GNF7
Organism   Xanthomonas oryzae pv. oryzae strain C2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2575870..2586907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKR89_RS11995 (WKR89_11960) - 2576291..2577487 (+) 1197 Protein_2295 hypothetical protein -
  WKR89_RS12000 (WKR89_11965) - 2577484..2578056 (+) 573 WP_011407966.1 DNA-3-methyladenine glycosylase I -
  WKR89_RS12005 (WKR89_11970) - 2578064..2578345 (+) 282 Protein_2297 DUF72 domain-containing protein -
  WKR89_RS12015 (WKR89_11980) - 2578680..2579354 (-) 675 WP_069960075.1 YitT family protein -
  WKR89_RS12020 (WKR89_11985) pilU 2579626..2580756 (-) 1131 WP_011407963.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WKR89_RS12025 (WKR89_11990) pilT 2580870..2581907 (-) 1038 WP_011258220.1 type IV pilus twitching motility protein PilT Machinery gene
  WKR89_RS12030 (WKR89_11995) - 2582271..2582963 (+) 693 WP_011407962.1 YggS family pyridoxal phosphate-dependent enzyme -
  WKR89_RS12035 (WKR89_12000) proC 2583007..2583867 (+) 861 WP_011407961.1 pyrroline-5-carboxylate reductase -
  WKR89_RS12040 (WKR89_12005) - 2583938..2585209 (+) 1272 WP_230455224.1 IS701 family transposase -
  WKR89_RS12045 (WKR89_12010) - 2585668..2586093 (+) 426 WP_011258216.1 HU family DNA-binding protein -
  WKR89_RS12050 (WKR89_12015) - 2586199..2586840 (+) 642 WP_042464628.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38678.38 Da        Isoelectric Point: 6.7747

>NTDB_id=962742 WKR89_RS12025 WP_011258220.1 2580870..2581907(-) (pilT) [Xanthomonas oryzae pv. oryzae strain C2]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEILSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVRRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=962742 WKR89_RS12025 WP_011258220.1 2580870..2581907(-) (pilT) [Xanthomonas oryzae pv. oryzae strain C2]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCGCTGGACCATAAGCAGGTGCATGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCGTTCGAAATTTTGTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCTTGATCCTGGTGACCGGCCCGACTGGTT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAATCAGCGCGAGGTGCGCCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCAGTGATTTCGCAGGC
GCTGCTGAAGAAAGTCGGCGGCGGGCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTCGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATTCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K0GNF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.71

0.786
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.517

95.362

0.672
  pilT Vibrio cholerae strain A1552

70.517

95.362

0.672
  pilT Neisseria meningitidis 8013

65.396

98.841

0.646
  pilT Neisseria gonorrhoeae MS11

65.103

98.841

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Acinetobacter baylyi ADP1

40.643

99.13

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394