Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WJ291_RS04220 Genome accession   NZ_CP149471
Coordinates   778142..779122 (+) Length   326 a.a.
NCBI ID   WP_080205543.1    Uniprot ID   -
Organism   Salmonella enterica strain 2024CK-1945753     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 773142..784122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ291_RS04190 (WJ291_04190) - 773720..774727 (+) 1008 Protein_726 DUF1202 domain-containing protein -
  WJ291_RS04195 (WJ291_04195) hemW 774818..775954 (-) 1137 WP_080205544.1 radical SAM family heme chaperone HemW -
  WJ291_RS04200 (WJ291_04200) - 775947..776540 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  WJ291_RS04205 (WJ291_04205) yggU 776548..776838 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  WJ291_RS04210 (WJ291_04210) - 776835..777401 (-) 567 WP_001094848.1 YggT family protein -
  WJ291_RS04215 (WJ291_04215) - 777420..778124 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  WJ291_RS04220 (WJ291_04220) pilT 778142..779122 (+) 981 WP_080205543.1 type IV pilus twitching motility protein PilT Machinery gene
  WJ291_RS04225 (WJ291_04225) - 779254..779940 (+) 687 WP_000098325.1 IclR family transcriptional regulator -
  WJ291_RS04230 (WJ291_04230) ruvX 779987..780403 (-) 417 WP_079981321.1 Holliday junction resolvase RuvX -
  WJ291_RS04235 (WJ291_04235) - 780403..780966 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  WJ291_RS04240 (WJ291_04240) gshB 781182..782129 (-) 948 WP_000593248.1 glutathione synthase -
  WJ291_RS04245 (WJ291_04245) rsmE 782149..782880 (-) 732 WP_001222489.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WJ291_RS04250 (WJ291_04250) endA 782957..783664 (-) 708 WP_000286123.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36111.34 Da        Isoelectric Point: 8.1072

>NTDB_id=959323 WJ291_RS04220 WP_080205543.1 778142..779122(+) (pilT) [Salmonella enterica strain 2024CK-1945753]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAKIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=959323 WJ291_RS04220 WP_080205543.1 778142..779122(+) (pilT) [Salmonella enterica strain 2024CK-1945753]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGCTGTCCTGATCTGGCGAAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACCACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TATCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGTGGCGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.245

97.853

0.374
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365
  pilU Pseudomonas stutzeri DSM 10701

35.65

100

0.362