NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	WJ127_RS03740	Genome accession	NZ_CP149468
Coordinates	777977..778957 (+)	Length	326 a.a.
NCBI ID	WP_080205543.1	Uniprot ID	-
Organism	Salmonella enterica strain 2024CK-1945744
Function	type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 772977..783957

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WJ127_RS03710 (WJ127_03705)	-	773555..774562 (+)	1008	Protein_727	DUF1202 domain-containing protein	-
WJ127_RS03715 (WJ127_03710)	hemW	774653..775789 (-)	1137	WP_080205544.1	radical SAM family heme chaperone HemW	-
WJ127_RS03720 (WJ127_03715)	-	775782..776375 (-)	594	WP_001174769.1	XTP/dITP diphosphatase	-
WJ127_RS03725 (WJ127_03720)	yggU	776383..776673 (-)	291	WP_001277205.1	DUF167 family protein YggU	-
WJ127_RS03730 (WJ127_03725)	-	776670..777236 (-)	567	WP_001094848.1	YggT family protein	-
WJ127_RS03735 (WJ127_03730)	-	777255..777959 (-)	705	WP_000997790.1	YggS family pyridoxal phosphate-dependent enzyme	-
WJ127_RS03740 (WJ127_03735)	pilT	777977..778957 (+)	981	WP_080205543.1	type IV pilus twitching motility protein PilT	Machinery gene
WJ127_RS03745 (WJ127_03740)	-	779089..779775 (+)	687	WP_000098325.1	IclR family transcriptional regulator	-
WJ127_RS03750 (WJ127_03745)	ruvX	779822..780238 (-)	417	WP_079981321.1	Holliday junction resolvase RuvX	-
WJ127_RS03755 (WJ127_03750)	-	780238..780801 (-)	564	WP_001053167.1	YqgE/AlgH family protein	-
WJ127_RS03760 (WJ127_03755)	gshB	781017..781964 (-)	948	WP_000593248.1	glutathione synthase	-
WJ127_RS03765 (WJ127_03760)	rsmE	781984..782715 (-)	732	WP_001222489.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
WJ127_RS03770 (WJ127_03765)	endA	782792..783499 (-)	708	WP_000286123.1	deoxyribonuclease I	-

Sequence

Protein

Download Length: 326 a.a. Molecular weight: 36111.34 Da Isoelectric Point: 8.1072

                                >NTDB_id=959301 WJ127_RS03740 WP_080205543.1 777977..778957(+) (pilT) [Salmonella enterica strain 2024CK-1945744]

MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA

SAFTHQQGTSLALRLLPERCPDLAKIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE

YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS

FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR

QAQGRL

Nucleotide

Download Length: 981 bp

                                >NTDB_id=959301 WJ127_RS03740 WP_080205543.1 777977..778957(+) (pilT) [Salmonella enterica strain 2024CK-1945744]

ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG

ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG

ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC

AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGCTGTCCTGATCTGGCGAAAATCCA

GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT

CCACCACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG

TATCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG

CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG

CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGTGGCGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT

TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT

CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA

AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT

CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.624	100	0.488
pilT	Vibrio cholerae strain A1552	48.624	100	0.488
pilT	Neisseria meningitidis 8013	46.951	100	0.472
pilT	Neisseria gonorrhoeae MS11	46.646	100	0.469
pilT	Acinetobacter baylyi ADP1	44.954	100	0.451
pilT	Pseudomonas stutzeri DSM 10701	44.954	100	0.451
pilT	Acinetobacter baumannii D1279779	44.954	100	0.451
pilT	Acinetobacter baumannii strain A118	44.954	100	0.451
pilT	Acinetobacter nosocomialis M2	44.648	100	0.448
pilT	Pseudomonas aeruginosa PAK	44.648	100	0.448
pilT	Legionella pneumophila strain Lp02	44.654	97.546	0.436
pilT	Legionella pneumophila strain ERS1305867	44.654	97.546	0.436
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	38.245	97.853	0.374
pilU	Acinetobacter baylyi ADP1	36.842	99.08	0.365
pilU	Pseudomonas stutzeri DSM 10701	35.65	100	0.362

Detailed information NTG0959301