Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WHX55_RS29160 Genome accession   NZ_CP148752
Coordinates   6385273..6386307 (-) Length   344 a.a.
NCBI ID   WP_353741741.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain AB1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6380273..6391307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHX55_RS29130 (WHX55_29100) ruvX 6380406..6380843 (+) 438 WP_103395762.1 Holliday junction resolvase RuvX -
  WHX55_RS29135 (WHX55_29105) pyrR 6380923..6381426 (+) 504 WP_223456707.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  WHX55_RS29140 (WHX55_29110) - 6381454..6382458 (+) 1005 WP_007939362.1 aspartate carbamoyltransferase catalytic subunit -
  WHX55_RS29145 (WHX55_29115) - 6382455..6383726 (+) 1272 WP_353741739.1 dihydroorotase -
  WHX55_RS29150 (WHX55_29120) - 6383841..6384269 (-) 429 WP_150725059.1 TM2 domain-containing protein -
  WHX55_RS29155 (WHX55_29125) - 6384521..6385141 (+) 621 WP_353741740.1 C40 family peptidase -
  WHX55_RS29160 (WHX55_29130) pilT 6385273..6386307 (-) 1035 WP_353741741.1 type IV pilus twitching motility protein PilT Machinery gene
  WHX55_RS29165 (WHX55_29135) - 6386364..6387050 (+) 687 WP_353741742.1 YggS family pyridoxal phosphate-dependent enzyme -
  WHX55_RS29170 (WHX55_29140) proC 6387085..6387903 (+) 819 WP_353741743.1 pyrroline-5-carboxylate reductase -
  WHX55_RS29175 (WHX55_29145) - 6387928..6388512 (+) 585 WP_223460574.1 YggT family protein -
  WHX55_RS29180 (WHX55_29150) - 6388512..6388808 (+) 297 WP_046039238.1 DUF167 domain-containing protein -
  WHX55_RS29185 (WHX55_29155) - 6388991..6390130 (+) 1140 WP_150725054.1 homoserine O-acetyltransferase -
  WHX55_RS29190 (WHX55_29160) metW 6390138..6390758 (+) 621 WP_150725053.1 methionine biosynthesis protein MetW -
  WHX55_RS29195 (WHX55_29165) - 6390785..6391219 (+) 435 WP_150725052.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37802.31 Da        Isoelectric Point: 6.9127

>NTDB_id=955461 WHX55_RS29160 WP_353741741.1 6385273..6386307(-) (pilT) [Pseudomonas fluorescens strain AB1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHQQVQALIHAIMNDTQRVDFEKHLETDFSFDVPGVAR
FRVNVFNQNRGAGAVFRTIPAKVQSMDELGMGDVFHKMTDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSAIQSGGSLGMQTLDMSL
KDLVTKGLVSRDHARETARSPDGF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=955461 WHX55_RS29160 WP_353741741.1 6385273..6386307(-) (pilT) [Pseudomonas fluorescens strain AB1]
ATGGATATCACCGAACTGCTGGCTTTCAGCGCCAAACAGGGCGCATCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATTCGGGTGGATGGCGATGTGCGGCGCATCAACCTGCCGCCCCTGGACCACCAGCAGGTGCAGGCACTGATCCATGCCA
TCATGAATGACACCCAGCGGGTGGATTTCGAAAAGCATCTGGAAACCGACTTTTCCTTCGACGTGCCCGGCGTGGCGCGC
TTTCGGGTCAATGTGTTCAACCAGAACCGTGGCGCGGGTGCCGTGTTTCGCACCATCCCGGCAAAAGTGCAGAGCATGGA
CGAACTGGGCATGGGCGACGTATTCCACAAGATGACCGACGCTCCCCGTGGCCTGGTGCTGGTGACCGGCCCGACCGGTT
CCGGCAAATCCACCACGCTCGCGGCGATGATCGATTACCTCAATACCCATCGTCATCACCACATCCTCACCATCGAGGAC
CCCATCGAGTTCGTTCACGAGTCGCGCAAATGCCTGATCAATCAGCGTGAAGTCCACCGCGATACCCGTAGTTTCGCCAC
CGCCTTGCGTTCGGCGCTGCGGGAAGACCCGGATGTGATCCTGGTGGGGGAGATGCGCGATCTGGAGACTATTCGCCTGG
CATTGACCGCGGCCGAAACCGGCCATCTGGTGTTCGGCACGCTGCACACCACGTCGGCAGCGAAAACCATCGATCGGGTG
GTCGACGTGTTTCCGGGGGACGAGAAGTCGATGGTGCGCTCGATGCTTTCCGAGTCGCTGCTGGCGGTGGTGTCCCAGAC
CCTGGTGAAGAAAATCGGCGGCGGACGCATCGCGGCCCACGAGATCATGCTGGGGACATCGGCAATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATTCAGTCGGGAGGGTCGTTGGGGATGCAGACGCTGGATATGAGCTTG
AAGGACCTGGTGACCAAGGGGTTGGTCAGTCGCGACCATGCGCGGGAGACGGCGCGGTCGCCGGATGGGTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

84.012

100

0.84
  pilT Pseudomonas stutzeri DSM 10701

83.14

100

0.831
  pilT Acinetobacter nosocomialis M2

75.872

100

0.759
  pilT Acinetobacter baumannii D1279779

75.872

100

0.759
  pilT Acinetobacter baumannii strain A118

75.872

100

0.759
  pilT Acinetobacter baylyi ADP1

75.291

100

0.753
  pilT Legionella pneumophila strain Lp02

68.895

100

0.689
  pilT Legionella pneumophila strain ERS1305867

68.895

100

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.781

95.64

0.648
  pilT Vibrio cholerae strain A1552

67.781

95.64

0.648
  pilT Neisseria meningitidis 8013

64.058

100

0.642
  pilT Neisseria gonorrhoeae MS11

63.768

100

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Vibrio cholerae strain A1552

40.233

99.709

0.401
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395