Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WDB99_RS07795 Genome accession   NZ_CP147991
Coordinates   1658766..1659788 (+) Length   340 a.a.
NCBI ID   WP_033632094.1    Uniprot ID   -
Organism   Serratia marcescens isolate 60-6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1653766..1664788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDB99_RS07755 (WDB99_07755) - 1653943..1654184 (+) 242 Protein_1487 DUF4160 domain-containing protein -
  WDB99_RS07760 (WDB99_07760) - 1654168..1654416 (+) 249 WP_004937422.1 DUF2442 domain-containing protein -
  WDB99_RS07765 (WDB99_07765) hemW 1654452..1655594 (-) 1143 WP_033632089.1 radical SAM family heme chaperone HemW -
  WDB99_RS07770 (WDB99_07770) - 1655587..1656180 (-) 594 WP_049239920.1 XTP/dITP diphosphatase -
  WDB99_RS07775 (WDB99_07775) yggU 1656321..1656614 (-) 294 WP_016930091.1 DUF167 family protein YggU -
  WDB99_RS07780 (WDB99_07780) - 1656611..1657165 (-) 555 WP_033632091.1 YggT family protein -
  WDB99_RS07785 (WDB99_07785) proC 1657197..1658018 (-) 822 WP_033632092.1 pyrroline-5-carboxylate reductase -
  WDB99_RS07790 (WDB99_07790) - 1658038..1658745 (-) 708 WP_015379048.1 YggS family pyridoxal phosphate-dependent enzyme -
  WDB99_RS07795 (WDB99_07795) pilT 1658766..1659788 (+) 1023 WP_033632094.1 type IV pilus twitching motility protein PilT Machinery gene
  WDB99_RS07800 (WDB99_07800) ruvX 1659785..1660207 (-) 423 WP_033632095.1 Holliday junction resolvase RuvX -
  WDB99_RS07805 (WDB99_07805) - 1660207..1660770 (-) 564 WP_004937452.1 YqgE/AlgH family protein -
  WDB99_RS07810 (WDB99_07810) gshB 1660916..1661866 (-) 951 WP_033632096.1 glutathione synthase -
  WDB99_RS07815 (WDB99_07815) rsmE 1661877..1662608 (-) 732 WP_085058862.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WDB99_RS07820 (WDB99_07820) endA 1662678..1663373 (-) 696 WP_015379043.1 deoxyribonuclease I -
  WDB99_RS07825 (WDB99_07825) - 1663475..1663987 (-) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36377.63 Da        Isoelectric Point: 7.2888

>NTDB_id=949897 WDB99_RS07795 WP_033632094.1 1658766..1659788(+) (pilT) [Serratia marcescens isolate 60-6]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGVSLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAAEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=949897 WDB99_RS07795 WP_033632094.1 1658766..1659788(+) (pilT) [Serratia marcescens isolate 60-6]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCATTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGAGCGGGGAA
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGAGTTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCAGCCATCGTCCCGGCGTTGCTGCGGCGCGCCGACGGATTGATTCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGTTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGGGCCCAGCTGGCCGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCTGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCGGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGAGTGCGCGGCGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Neisseria gonorrhoeae MS11

49.702

98.824

0.491
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Legionella pneumophila strain ERS1305867

48.955

98.529

0.482
  pilT Legionella pneumophila strain Lp02

48.955

98.529

0.482
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Pseudomonas stutzeri DSM 10701

47.917

98.824

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.322

100

0.456
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.279

99.118

0.379