Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WCY20_RS06860 Genome accession   NZ_CP147919
Coordinates   1363331..1364407 (-) Length   358 a.a.
NCBI ID   WP_345978071.1    Uniprot ID   -
Organism   Sulfurimonas sp. HSL3-7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1358331..1369407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCY20_RS06835 (WCY20_06835) lysS 1358647..1360167 (-) 1521 WP_345978065.1 lysine--tRNA ligase -
  WCY20_RS06840 (WCY20_06840) - 1360188..1360667 (-) 480 WP_345978067.1 Fur family transcriptional regulator -
  WCY20_RS06845 (WCY20_06845) - 1360670..1361335 (-) 666 WP_345978069.1 CvpA family protein -
  WCY20_RS06850 (WCY20_06850) - 1361537..1362889 (+) 1353 WP_345978227.1 3-deoxy-7-phosphoheptulonate synthase class II -
  WCY20_RS06855 (WCY20_06855) - 1363020..1363310 (+) 291 WP_345978229.1 DUF3293 domain-containing protein -
  WCY20_RS06860 (WCY20_06860) pilT 1363331..1364407 (-) 1077 WP_345978071.1 type IV pilus twitching motility protein PilT Machinery gene
  WCY20_RS06865 (WCY20_06865) - 1364523..1364864 (-) 342 WP_345978073.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  WCY20_RS06870 (WCY20_06870) gatC 1364868..1365158 (-) 291 WP_345978075.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  WCY20_RS06875 (WCY20_06875) - 1365301..1365873 (+) 573 WP_345978076.1 hypothetical protein -
  WCY20_RS06880 (WCY20_06880) - 1365876..1366868 (+) 993 WP_345978078.1 hypothetical protein -
  WCY20_RS06885 (WCY20_06885) - 1366872..1367687 (+) 816 WP_345978080.1 tyrosine-type recombinase/integrase -
  WCY20_RS06890 (WCY20_06890) - 1367909..1368229 (+) 321 WP_345978082.1 hypothetical protein -
  WCY20_RS06895 (WCY20_06895) - 1368226..1368837 (+) 612 WP_345978084.1 type III pantothenate kinase -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39833.85 Da        Isoelectric Point: 6.4001

>NTDB_id=949738 WCY20_RS06860 WP_345978071.1 1363331..1364407(-) (pilT) [Sulfurimonas sp. HSL3-7]
MSQALDIRKLLKSVVAYKASDLHLVSRSEPQIRIDGRLVALNLPTLDGKMIEDMAYSLLTEKQKKEFEEHGELDFAIMLP
EIGRFRANYYKTLEDLACAFRIIPIDIPSLDDLNANPAFKKIIQREKGLILVTGPTGSGKSTTLAAMLNEINETENRHII
TVEDPVEFIHNNKKSLFSQRNVGNDTKSFAIALKYAMRQDPDVILIGEMRDQETIGAALTAAETGHLVFGTLHTNSAPQT
INRIIDVFPGEVQPQIRAQLSTSLVAVIAQALLPKIGGGRIASQEFMLTNPAIANLIREDKVHQVYSQMQLNQSETGMVT
QTQQLVEFVQKRIVTRNIAVAYSNRADELIRILESSGL

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=949738 WCY20_RS06860 WP_345978071.1 1363331..1364407(-) (pilT) [Sulfurimonas sp. HSL3-7]
ATGAGTCAAGCATTAGATATTAGAAAATTATTAAAAAGTGTTGTTGCCTATAAAGCATCGGATCTTCACCTTGTCTCGCG
TTCTGAACCTCAGATTCGTATTGACGGCCGTTTGGTCGCACTGAACCTGCCGACGCTCGACGGCAAGATGATCGAAGATA
TGGCCTACTCACTGTTGACCGAAAAGCAGAAAAAAGAGTTTGAAGAGCATGGCGAACTTGACTTTGCGATCATGCTGCCG
GAGATCGGGCGTTTCCGTGCCAACTACTATAAAACGTTAGAAGATCTTGCCTGTGCCTTCCGTATCATTCCTATCGATAT
TCCGAGTCTTGATGACCTTAACGCAAATCCTGCATTTAAAAAGATCATTCAACGCGAAAAAGGTCTGATCCTTGTCACTG
GTCCGACCGGTTCCGGTAAATCGACCACACTGGCGGCGATGCTCAACGAGATCAATGAGACGGAGAACAGACACATCATT
ACCGTTGAAGACCCGGTCGAGTTCATTCATAACAATAAAAAAAGTCTCTTCTCCCAACGTAATGTCGGTAATGACACCAA
AAGTTTCGCCATAGCTCTCAAATATGCGATGCGTCAGGATCCCGATGTCATCCTCATCGGGGAGATGCGTGACCAGGAGA
CAATCGGTGCAGCATTGACCGCAGCGGAGACAGGTCACCTTGTATTTGGGACACTTCACACCAACTCGGCACCGCAGACG
ATCAACCGTATTATCGATGTTTTCCCAGGCGAAGTGCAGCCACAAATACGGGCGCAGCTCTCAACATCACTGGTTGCCGT
TATTGCGCAGGCGCTGCTGCCGAAGATCGGCGGGGGGCGTATCGCTTCACAGGAATTTATGCTGACAAACCCTGCAATTG
CCAACCTTATCCGTGAAGACAAGGTCCACCAGGTCTATTCACAAATGCAATTGAATCAGTCAGAGACCGGTATGGTGACA
CAGACGCAGCAACTTGTCGAATTTGTACAGAAACGGATTGTAACCAGAAACATAGCTGTCGCTTATTCCAACCGTGCGGA
TGAGCTTATCCGTATTCTTGAATCTTCCGGGTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

52.727

92.179

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.727

92.179

0.486
  pilT Legionella pneumophila strain Lp02

51.94

93.575

0.486
  pilT Legionella pneumophila strain ERS1305867

51.94

93.575

0.486
  pilT Pseudomonas stutzeri DSM 10701

51.045

93.575

0.478
  pilT Acinetobacter baumannii strain A118

50.746

93.575

0.475
  pilT Acinetobacter nosocomialis M2

50.746

93.575

0.475
  pilT Acinetobacter baumannii D1279779

50.746

93.575

0.475
  pilT Acinetobacter baylyi ADP1

50.909

92.179

0.469
  pilT Pseudomonas aeruginosa PAK

50.149

93.575

0.469
  pilT Neisseria meningitidis 8013

49.405

93.855

0.464
  pilT Neisseria gonorrhoeae MS11

50.151

92.458

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.152

100

0.455
  pilU Acinetobacter baylyi ADP1

39.481

96.927

0.383
  pilU Vibrio cholerae strain A1552

37.216

98.324

0.366
  pilU Pseudomonas stutzeri DSM 10701

37.861

96.648

0.366