Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WA016_RS26210 Genome accession   NZ_CP147913
Coordinates   6616081..6617193 (+) Length   370 a.a.
NCBI ID   WP_338864178.1    Uniprot ID   -
Organism   Myxococcus stipitatus strain KYC2006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6611081..6622193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WA016_RS26190 (WA016_05299) - 6611355..6612398 (-) 1044 WP_338864174.1 diguanylate cyclase -
  WA016_RS26195 (WA016_05300) trmB 6612508..6613128 (-) 621 WP_338864175.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  WA016_RS26200 (WA016_05301) - 6613198..6614127 (+) 930 WP_338864176.1 bifunctional riboflavin kinase/FAD synthetase -
  WA016_RS26205 (WA016_05302) pilB 6614342..6616042 (+) 1701 WP_338864177.1 type IV-A pilus assembly ATPase PilB -
  WA016_RS26210 (WA016_05303) pilT 6616081..6617193 (+) 1113 WP_338864178.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WA016_RS26215 (WA016_05304) - 6617251..6618504 (+) 1254 WP_338864179.1 type II secretion system F family protein -
  WA016_RS26220 (WA016_05305) - 6618521..6620104 (+) 1584 WP_338864180.1 ATP-binding protein -
  WA016_RS26225 (WA016_05306) - 6620152..6621540 (+) 1389 WP_338873795.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40490.82 Da        Isoelectric Point: 8.2933

>NTDB_id=949675 WA016_RS26210 WP_338864178.1 6616081..6617193(+) (pilT) [Myxococcus stipitatus strain KYC2006]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEDNELDLSFGVKGLS
RFRANIFMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQLSFVLEGVMSQALVAKAGAPGRVLALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKFGMQTFN
QALAALLLRRVITQDEAFGRSSDAEELRNILATGGGMPVGQRPAGGPAGR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=949675 WA016_RS26210 WP_338864178.1 6616081..6617193(+) (pilT) [Myxococcus stipitatus strain KYC2006]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACGTCACCACCGGCTCTCCGCC
GCAGCTGCGCGTGGACGGCGAGCTTGTCCCCTTGAAGACGGCGCCGTTGACGCCCGTGGAGACCAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGACAACGAGCTGGACTTGTCCTTCGGCGTGAAGGGCCTGTCG
CGCTTCCGCGCCAACATCTTCATGCAGCGTGGCGCGGTGGCCGGGGCCTTCCGCACCATTCCCTTCAAGATTCTGACCTT
CCAGGAGCTGGGCCTGCCGCCCGTGGTCGCCGAGCTGGTGAAGAAGCCGCGCGGCCTCATCCTGGTGACGGGCCCCACGG
GCTCGGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCACACAAGAACTGCCTAGTGAACCAGCGCGAGGTGGGAGCGGACACTCGAAACTTCAA
GACGGCGCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTGCTGGTGGGTGAGCTCCGAGACCTCGAGACCATCGAAG
CGGCGCTCACCATCGCGGAGACGGGCCACATCTGCTACGCGACGCTGCACACTAACAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTCTTCCCGCCGTACCAGCAGCCGCAGGTGCGCGCCCAGCTCTCCTTCGTGCTGGAGGGCGTGATGAGTCA
GGCGCTGGTGGCCAAGGCGGGCGCTCCGGGCCGCGTGCTGGCCCTGGAGGTCATGGTGCCCAACCCCGCCATCCGGAACC
TCATCCGCGAGGACAAGGTCCATCAGATCTACTCCTCCATGCAGGTGGGTCAGGCGAAGTTCGGCATGCAGACCTTCAAC
CAGGCGCTCGCCGCGCTGCTCCTGCGGCGGGTCATCACCCAGGATGAGGCCTTCGGACGCTCCAGTGATGCGGAAGAGCT
GCGCAACATCCTGGCCACGGGCGGTGGAATGCCTGTGGGACAGAGGCCCGCTGGCGGGCCGGCGGGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

93.243

0.481
  pilT Legionella pneumophila strain Lp02

52.836

90.541

0.478
  pilT Legionella pneumophila strain ERS1305867

52.836

90.541

0.478
  pilT Pseudomonas aeruginosa PAK

51.94

90.541

0.47
  pilT Pseudomonas stutzeri DSM 10701

51.94

90.541

0.47
  pilT Acinetobacter nosocomialis M2

52.266

89.459

0.468
  pilT Acinetobacter baumannii D1279779

52.266

89.459

0.468
  pilT Acinetobacter baumannii strain A118

52.266

89.459

0.468
  pilT Acinetobacter baylyi ADP1

52.121

89.189

0.465
  pilT Vibrio cholerae strain A1552

48.802

90.27

0.441
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.802

90.27

0.441
  pilT Neisseria meningitidis 8013

44.575

92.162

0.411
  pilT Neisseria gonorrhoeae MS11

44.575

92.162

0.411
  pilU Vibrio cholerae strain A1552

42.577

96.486

0.411
  pilU Pseudomonas stutzeri DSM 10701

41.026

94.865

0.389
  pilU Acinetobacter baylyi ADP1

39.481

93.784

0.37