Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WCU29_RS20590 Genome accession   NZ_CP147621
Coordinates   4310090..4311112 (-) Length   340 a.a.
NCBI ID   WP_060430992.1    Uniprot ID   -
Organism   Serratia marcescens strain GN02966     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4305090..4316112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU29_RS20560 (WCU29_20555) - 4305891..4306403 (+) 513 WP_060429432.1 SprT family zinc-dependent metalloprotease -
  WCU29_RS20565 (WCU29_20560) endA 4306505..4307200 (+) 696 WP_033649591.1 deoxyribonuclease I -
  WCU29_RS20570 (WCU29_20565) rsmE 4307270..4308001 (+) 732 WP_060430995.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WCU29_RS20575 (WCU29_20570) gshB 4308012..4308962 (+) 951 WP_047729854.1 glutathione synthase -
  WCU29_RS20580 (WCU29_20575) - 4309108..4309671 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  WCU29_RS20585 (WCU29_20580) ruvX 4309671..4310093 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  WCU29_RS20590 (WCU29_20585) pilT 4310090..4311112 (-) 1023 WP_060430992.1 type IV pilus twitching motility protein PilT Machinery gene
  WCU29_RS20595 (WCU29_20590) - 4311133..4311840 (+) 708 WP_060426992.1 YggS family pyridoxal phosphate-dependent enzyme -
  WCU29_RS20600 (WCU29_20595) proC 4311860..4312681 (+) 822 WP_033649586.1 pyrroline-5-carboxylate reductase -
  WCU29_RS20605 (WCU29_20600) - 4312713..4313267 (+) 555 WP_033635944.1 YggT family protein -
  WCU29_RS20610 (WCU29_20605) yggU 4313264..4313554 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  WCU29_RS20615 (WCU29_20610) - 4313572..4314165 (+) 594 WP_060388227.1 XTP/dITP diphosphatase -
  WCU29_RS20620 (WCU29_20615) hemW 4314158..4315300 (+) 1143 WP_060388228.1 radical SAM family heme chaperone HemW -
  WCU29_RS20625 (WCU29_20620) - 4315337..4315423 (-) 87 Protein_4024 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36444.72 Da        Isoelectric Point: 6.8881

>NTDB_id=947653 WCU29_RS20590 WP_060430992.1 4310090..4311112(-) (pilT) [Serratia marcescens strain GN02966]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAVPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGESAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=947653 WCU29_RS20590 WP_060430992.1 4310090..4311112(-) (pilT) [Serratia marcescens strain GN02966]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGCCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACCGGCCGGGCGGGGAA
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCAGGGATGTCTCTCGCGTTGCGGCGCATCGCCGGGCAGGCGCCCTCGCT
TGCCGAGCTGGCGGTGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGGCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGTGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGAGTGCGGGGGAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Pseudomonas aeruginosa PAK

49.555

99.118

0.491
  pilT Neisseria meningitidis 8013

50.301

97.647

0.491
  pilT Neisseria gonorrhoeae MS11

49.702

98.824

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.961

99.118

0.485
  pilT Legionella pneumophila strain ERS1305867

49.398

97.647

0.482
  pilT Legionella pneumophila strain Lp02

49.398

97.647

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.82

100

0.459
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371