Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WCU10_RS21095 Genome accession   NZ_CP147539
Coordinates   4435623..4436645 (-) Length   340 a.a.
NCBI ID   WP_338808139.1    Uniprot ID   -
Organism   Serratia marcescens strain GN03438     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4430623..4441645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU10_RS21065 (WCU10_21065) - 4431427..4431939 (+) 513 WP_033654666.1 SprT family zinc-dependent metalloprotease -
  WCU10_RS21070 (WCU10_21070) endA 4432041..4432736 (+) 696 WP_033649591.1 deoxyribonuclease I -
  WCU10_RS21075 (WCU10_21075) rsmE 4432806..4433537 (+) 732 WP_033654665.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WCU10_RS21080 (WCU10_21080) gshB 4433548..4434498 (+) 951 WP_033635939.1 glutathione synthase -
  WCU10_RS21085 (WCU10_21085) - 4434641..4435204 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  WCU10_RS21090 (WCU10_21090) ruvX 4435204..4435626 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  WCU10_RS21095 (WCU10_21095) pilT 4435623..4436645 (-) 1023 WP_338808139.1 type IV pilus twitching motility protein PilT Machinery gene
  WCU10_RS21100 (WCU10_21100) - 4436666..4437373 (+) 708 WP_338808140.1 YggS family pyridoxal phosphate-dependent enzyme -
  WCU10_RS21105 (WCU10_21105) proC 4437393..4438214 (+) 822 WP_033635943.1 pyrroline-5-carboxylate reductase -
  WCU10_RS21110 (WCU10_21110) - 4438246..4438800 (+) 555 WP_033635944.1 YggT family protein -
  WCU10_RS21115 (WCU10_21115) yggU 4438797..4439087 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  WCU10_RS21120 (WCU10_21120) - 4439105..4439698 (+) 594 WP_033635946.1 XTP/dITP diphosphatase -
  WCU10_RS21125 (WCU10_21125) hemW 4439691..4440833 (+) 1143 WP_338808141.1 radical SAM family heme chaperone HemW -
  WCU10_RS21130 (WCU10_21130) - 4440870..4440956 (-) 87 Protein_4121 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36413.72 Da        Isoelectric Point: 6.7770

>NTDB_id=946786 WCU10_RS21095 WP_338808139.1 4435623..4436645(-) (pilT) [Serratia marcescens strain GN03438]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQHESLRRLGQVDLALHRPGGE
RLRVNVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMKRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=946786 WCU10_RS21095 WP_338808139.1 4435623..4436645(-) (pilT) [Serratia marcescens strain GN03438]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCACGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGGGCGGGGAG
CGGCTGCGGGTCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCCTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGTTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGACGTTCGCTGATCCAGCAGCGGGAAATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGGCATCTGGTGCTGGCGACGTTGCACACCCGCAGTGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAAGCGGCCCGGTGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGTGCGGGGGAGGGAACGGAAGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

55.152

97.059

0.535
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.152

97.059

0.535
  pilT Acinetobacter baylyi ADP1

51.662

97.353

0.503
  pilT Neisseria meningitidis 8013

50.904

97.647

0.497
  pilT Acinetobacter baumannii D1279779

51.212

97.059

0.497
  pilT Acinetobacter nosocomialis M2

51.212

97.059

0.497
  pilT Acinetobacter baumannii strain A118

51.212

97.059

0.497
  pilT Neisseria gonorrhoeae MS11

50.602

97.647

0.494
  pilT Legionella pneumophila strain ERS1305867

49.699

97.647

0.485
  pilT Legionella pneumophila strain Lp02

49.699

97.647

0.485
  pilT Pseudomonas aeruginosa PAK

50

97.059

0.485
  pilT Pseudomonas stutzeri DSM 10701

49.394

97.059

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.101

100

0.462
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371