Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WCU31_RS20670 Genome accession   NZ_CP147521
Coordinates   4256456..4257478 (-) Length   340 a.a.
NCBI ID   WP_338858685.1    Uniprot ID   -
Organism   Serratia marcescens strain GN02777     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4251456..4262478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU31_RS20640 (WCU31_20640) - 4252257..4252769 (+) 513 WP_308322106.1 SprT family zinc-dependent metalloprotease -
  WCU31_RS20645 (WCU31_20645) endA 4252871..4253566 (+) 696 WP_038879275.1 deoxyribonuclease I -
  WCU31_RS20650 (WCU31_20650) rsmE 4253636..4254367 (+) 732 WP_174136631.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WCU31_RS20655 (WCU31_20655) gshB 4254378..4255328 (+) 951 WP_038879277.1 glutathione synthase -
  WCU31_RS20660 (WCU31_20660) - 4255474..4256037 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  WCU31_RS20665 (WCU31_20665) ruvX 4256037..4256459 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  WCU31_RS20670 (WCU31_20670) pilT 4256456..4257478 (-) 1023 WP_338858685.1 type IV pilus twitching motility protein PilT Machinery gene
  WCU31_RS20675 (WCU31_20675) - 4257499..4258206 (+) 708 WP_192974193.1 YggS family pyridoxal phosphate-dependent enzyme -
  WCU31_RS20680 (WCU31_20680) proC 4258226..4259047 (+) 822 WP_126180722.1 pyrroline-5-carboxylate reductase -
  WCU31_RS20685 (WCU31_20685) - 4259079..4259633 (+) 555 WP_033635944.1 YggT family protein -
  WCU31_RS20690 (WCU31_20690) yggU 4259630..4259920 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  WCU31_RS20695 (WCU31_20695) - 4259938..4260531 (+) 594 WP_338858686.1 XTP/dITP diphosphatase -
  WCU31_RS20700 (WCU31_20700) hemW 4260524..4261666 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  WCU31_RS20705 (WCU31_20705) - 4261702..4261950 (-) 249 WP_038879292.1 DUF2442 domain-containing protein -
  WCU31_RS20710 (WCU31_20710) - 4261934..4262164 (-) 231 WP_240023512.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36458.81 Da        Isoelectric Point: 6.8839

>NTDB_id=946627 WCU31_RS20670 WP_338858685.1 4256456..4257478(-) (pilT) [Serratia marcescens strain GN02777]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQIDLVLHRPGGE
RLRANVFQQSAGMSLALRRIAGQVPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDVATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGD

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=946627 WCU31_RS20670 WP_338858685.1 4256456..4257478(-) (pilT) [Serratia marcescens strain GN02777]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTACTGAATGCGCAGCAGCGCGAGAGTCTGCGGCGGCTGGGGCAGATCGATCTGGTGCTGCACAGGCCGGGTGGGGAG
CGGCTGCGAGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGTGCCTTCGCT
TGCCGAGCTGGCGGCCCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATTCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATGTCGCCACCATTCGGT
TGGCGCTCACTGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAAAGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGTTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(68-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.662

97.353

0.503
  pilT Neisseria meningitidis 8013

50.298

98.824

0.497
  pilT Acinetobacter baumannii strain A118

51.212

97.059

0.497
  pilT Acinetobacter nosocomialis M2

51.212

97.059

0.497
  pilT Acinetobacter baumannii D1279779

51.212

97.059

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

97.647

0.491
  pilT Pseudomonas aeruginosa PAK

50

97.059

0.485
  pilT Legionella pneumophila strain ERS1305867

49.547

97.353

0.482
  pilT Legionella pneumophila strain Lp02

49.547

97.353

0.482
  pilT Pseudomonas stutzeri DSM 10701

49.394

97.059

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.322

100

0.456
  pilU Vibrio cholerae strain A1552

39.815

95.294

0.379
  pilU Acinetobacter baylyi ADP1

39.628

95

0.376
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368