Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WAC51_RS02645 Genome accession   NZ_CP146845
Coordinates   575682..576809 (-) Length   375 a.a.
NCBI ID   WP_049455379.1    Uniprot ID   A0A3S0HDZ5
Organism   Stenotrophomonas geniculata strain WXY53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 570682..581809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAC51_RS02625 (WAC51_02625) - 571142..572131 (+) 990 WP_049434945.1 fumarylacetoacetate hydrolase family protein -
  WAC51_RS02630 (WAC51_02630) maiA 572146..572829 (+) 684 WP_338713206.1 maleylacetoacetate isomerase -
  WAC51_RS02635 (WAC51_02635) - 572863..574359 (-) 1497 WP_338713207.1 UdgX family uracil-DNA binding protein -
  WAC51_RS02640 (WAC51_02640) - 574340..575623 (-) 1284 WP_126560922.1 putative DNA modification/repair radical SAM protein -
  WAC51_RS02645 (WAC51_02645) pilU 575682..576809 (-) 1128 WP_049455379.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WAC51_RS02650 (WAC51_02650) - 576931..577296 (+) 366 WP_010480631.1 DUF4398 domain-containing protein -
  WAC51_RS02655 (WAC51_02655) - 577293..578177 (+) 885 WP_010480632.1 hypothetical protein -
  WAC51_RS02660 (WAC51_02660) - 578502..578738 (+) 237 WP_005407964.1 YdcH family protein -
  WAC51_RS02665 (WAC51_02665) - 579039..580409 (+) 1371 WP_010480640.1 pyridoxal-phosphate dependent enzyme -
  WAC51_RS02670 (WAC51_02670) - 580533..581705 (+) 1173 WP_338713209.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41370.65 Da        Isoelectric Point: 6.3738

>NTDB_id=944654 WAC51_RS02645 WP_049455379.1 575682..576809(-) (pilU) [Stenotrophomonas geniculata strain WXY53]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKAGTIDQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=944654 WAC51_RS02645 WP_049455379.1 575682..576809(-) (pilU) [Stenotrophomonas geniculata strain WXY53]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCCTGACCACCGGAGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTTTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGGAAACGATGGAGGCCG
CCATCGCTTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGCCGTCGCCTGCCGGCCACCGAAGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GTCGTGGCCAGGTGCACGAGATCAAGGCAGCAATGGAAGGCTCACTGGAGGAAGGCATGGAGAGTTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCACCATCGATCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAACACGACCCGTACGCGGATTTCTCGGCCGGCGCGCCGAGCATCACCCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S0HDZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.471

90.667

0.376
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

41.141

88.8

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.8

0.365
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363