Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V9W64_RS06000 Genome accession   NZ_CP146598
Coordinates   1297657..1298697 (+) Length   346 a.a.
NCBI ID   WP_274585765.1    Uniprot ID   A0A9X4IBT1
Organism   Neisseria leonii strain 51.81     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1292657..1303697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9W64_RS05975 (V9W64_05975) lpxC 1292698..1293618 (-) 921 WP_274585785.1 UDP-3-O-acyl-N-acetylglucosamine deacetylase -
  V9W64_RS05980 (V9W64_05980) gap 1294079..1295086 (+) 1008 WP_274585768.1 type I glyceraldehyde-3-phosphate dehydrogenase -
  V9W64_RS05985 (V9W64_05985) dksA 1295353..1295769 (+) 417 WP_274572504.1 RNA polymerase-binding protein DksA -
  V9W64_RS05990 (V9W64_05990) proC 1295864..1296679 (-) 816 WP_274585767.1 pyrroline-5-carboxylate reductase -
  V9W64_RS05995 (V9W64_05995) - 1296873..1297565 (-) 693 WP_274585766.1 YggS family pyridoxal phosphate-dependent enzyme -
  V9W64_RS06000 (V9W64_06000) pilT 1297657..1298697 (+) 1041 WP_274585765.1 type IV pilus twitching motility protein PilT Machinery gene
  V9W64_RS06005 (V9W64_06005) pdxA 1298841..1299836 (+) 996 WP_274585764.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
  V9W64_RS06010 (V9W64_06010) - 1299871..1300104 (+) 234 WP_274585763.1 cytochrome-c oxidase -
  V9W64_RS06015 (V9W64_06015) - 1300365..1301192 (+) 828 WP_274585762.1 ABC transporter ATP-binding protein -
  V9W64_RS06020 (V9W64_06020) mlaE 1301196..1301972 (+) 777 WP_274585761.1 lipid asymmetry maintenance ABC transporter permease subunit MlaE -
  V9W64_RS06025 (V9W64_06025) mlaD 1301978..1302496 (+) 519 WP_274585760.1 outer membrane lipid asymmetry maintenance protein MlaD -
  V9W64_RS06030 (V9W64_06030) - 1302570..1303163 (+) 594 WP_274585759.1 phospholipid-binding protein MlaC -
  V9W64_RS06035 (V9W64_06035) - 1303163..1303438 (+) 276 WP_274585758.1 lipid asymmetry maintenance protein MlaB -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 36740.24 Da        Isoelectric Point: 7.0817

>NTDB_id=943264 V9W64_RS06000 WP_274585765.1 1297657..1298697(+) (pilT) [Neisseria leonii strain 51.81]
MADLVGFLLEAAQKGASDVHLAAGEPAVLRIDGSLTRTAQAVWTAEQIENAVLPLMNEAQRTVWRDGGEVDFAFVLPDNK
RCRANVFQTASGAAAALRLIADNIPTLAGLGVPPVVHKLAELPRGLVLVTGPTGSGKSTTLAAMIDALNRSRPLHILTIE
DPIEFVHTNRTALIRQRALHEHTKSFAAALKSALREDLDVILVGELRDRETVRLALTAAETGHLVLATLHTTGAAKTIDR
IIDVFEAGEKDSVRAMLSESLRAVVSQLLLPALGGGRVAAHEILMATPAVRHLIRENKTAQIANAIQTGQAAGMQTLAQS
LQQLLHSGRIDAQTAARFGGSESGLI

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=943264 V9W64_RS06000 WP_274585765.1 1297657..1298697(+) (pilT) [Neisseria leonii strain 51.81]
ATGGCGGACTTGGTCGGTTTTCTATTGGAAGCGGCGCAAAAAGGCGCATCCGATGTCCATCTGGCGGCGGGCGAACCGGC
CGTTTTGCGTATCGACGGCAGCCTGACGCGTACCGCACAGGCGGTATGGACGGCAGAGCAAATCGAAAATGCCGTTTTGC
CGCTGATGAATGAGGCACAGCGTACCGTCTGGCGCGATGGCGGCGAAGTCGATTTTGCTTTTGTGCTGCCCGACAACAAA
CGGTGCCGTGCCAATGTTTTTCAGACGGCCTCCGGTGCGGCCGCCGCGTTGCGCCTGATTGCGGACAATATTCCGACATT
GGCCGGTTTGGGCGTACCGCCGGTGGTGCACAAGTTGGCCGAACTGCCGCGCGGTTTGGTGTTGGTAACCGGCCCCACCG
GCTCGGGCAAGTCCACCACGCTGGCGGCCATGATTGACGCGCTCAACCGCAGCCGGCCGCTGCATATTCTGACGATTGAA
GACCCGATCGAATTTGTCCATACCAACCGTACGGCATTAATCCGCCAGCGCGCGCTGCACGAGCACACCAAAAGTTTCGC
TGCCGCGCTCAAATCAGCGTTACGCGAAGACCTCGATGTGATTCTGGTGGGCGAATTGCGCGACCGCGAAACCGTCCGCC
TGGCTTTAACGGCAGCCGAAACCGGCCATCTGGTGCTGGCCACGCTGCATACCACCGGCGCGGCGAAAACGATAGACCGC
ATTATCGATGTGTTTGAAGCGGGCGAAAAAGACAGCGTACGCGCCATGTTGTCCGAAAGCCTGCGCGCCGTAGTATCGCA
GCTTTTGCTGCCCGCGCTCGGCGGCGGGCGCGTGGCGGCGCACGAAATTCTGATGGCGACACCGGCGGTGCGCCATTTAA
TCCGCGAGAACAAAACGGCGCAAATCGCCAATGCCATTCAAACCGGACAGGCCGCCGGTATGCAGACTTTGGCACAGTCA
TTGCAGCAGCTGCTGCACAGCGGCCGTATCGATGCGCAAACCGCCGCCCGTTTCGGCGGCAGCGAAAGCGGTTTGATATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

58.284

97.688

0.569
  pilT Neisseria meningitidis 8013

57.988

97.688

0.566
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

56.798

95.665

0.543
  pilT Vibrio cholerae strain A1552

56.798

95.665

0.543
  pilT Acinetobacter baylyi ADP1

56.156

96.243

0.54
  pilT Acinetobacter baumannii D1279779

55.556

96.243

0.535
  pilT Acinetobacter baumannii strain A118

55.556

96.243

0.535
  pilT Acinetobacter nosocomialis M2

55.556

96.243

0.535
  pilT Pseudomonas stutzeri DSM 10701

54.655

96.243

0.526
  pilT Pseudomonas aeruginosa PAK

54.054

96.243

0.52
  pilT Legionella pneumophila strain Lp02

53.731

96.821

0.52
  pilT Legionella pneumophila strain ERS1305867

53.731

96.821

0.52
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.231

93.931

0.462
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.532

0.387
  pilU Acinetobacter baylyi ADP1

37.101

99.711

0.37
  pilU Vibrio cholerae strain A1552

38.941

92.775

0.361