Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAEM60_RS16470 Genome accession   NZ_CP145886
Coordinates   3227498..3228535 (-) Length   345 a.a.
NCBI ID   WP_299738786.1    Uniprot ID   -
Organism   Rossellomorea sp. y25     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3222498..3233535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEM60_RS16440 (V2W31_16440) - 3222668..3223438 (-) 771 WP_299738775.1 hypothetical protein -
  AAEM60_RS16445 (V2W31_16445) - 3223435..3224052 (-) 618 WP_341356678.1 hypothetical protein -
  AAEM60_RS16450 (V2W31_16450) pilM 3224036..3225007 (-) 972 WP_341356679.1 pilus assembly protein PilM -
  AAEM60_RS16455 (V2W31_16455) - 3225029..3225775 (-) 747 WP_299738779.1 A24 family peptidase -
  AAEM60_RS16460 (V2W31_16460) - 3225777..3226166 (-) 390 WP_299738781.1 type II secretion system protein -
  AAEM60_RS16465 (V2W31_16465) - 3226284..3227495 (-) 1212 WP_299738784.1 type II secretion system F family protein -
  AAEM60_RS16470 (V2W31_16470) pilT 3227498..3228535 (-) 1038 WP_299738786.1 type IV pilus twitching motility protein PilT Machinery gene
  AAEM60_RS16475 (V2W31_16475) - 3228550..3230208 (-) 1659 WP_341356680.1 ATPase, T2SS/T4P/T4SS family -
  AAEM60_RS16480 (V2W31_16480) - 3230234..3231292 (-) 1059 WP_341356681.1 hypothetical protein -
  AAEM60_RS16485 (V2W31_16485) - 3231554..3233266 (-) 1713 WP_299738792.1 sensor domain-containing diguanylate cyclase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38668.62 Da        Isoelectric Point: 7.6954

>NTDB_id=940917 AAEM60_RS16470 WP_299738786.1 3227498..3228535(-) (pilT) [Rossellomorea sp. y25]
MKERIDHLLREAFESKASDIHLTIGSAPVFRIHGDLKRYGQDVLKPEDTEQMAKTIVPEHLWTEFKERGEMDFSYALPGV
SRFRVNAYFQRSCISIALRVVPRNIPTLEELKLPPVIMKIAEKHQGLVLVTGPTGSGKSTTLASMIHYMNQTMRKHIITL
EDPIEYLHKHGSSIIDQREVGFDTKSFAYGLRSALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTSSAVSTIE
RIIDVFPAEQQSQVRVQLATVLQGVIAQRLLPTVDKKGRIAVSEILLNNSAVANLIRSEKIHQIPTIMQTSKAQGMELFE
SNVSRYLHAGMISKEVASPFLQENR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=940917 AAEM60_RS16470 WP_299738786.1 3227498..3228535(-) (pilT) [Rossellomorea sp. y25]
TTGAAGGAACGGATAGATCATCTATTACGGGAGGCATTTGAATCAAAGGCCTCTGATATTCATTTAACGATAGGATCAGC
CCCTGTTTTCCGAATTCATGGAGACTTAAAGAGATATGGGCAGGATGTGCTGAAGCCGGAAGATACAGAGCAAATGGCAA
AAACCATTGTTCCCGAGCATTTATGGACGGAATTCAAGGAACGGGGGGAAATGGACTTTTCTTATGCCCTCCCAGGTGTC
TCACGGTTCCGTGTGAATGCCTATTTTCAACGTTCTTGTATTTCGATTGCATTACGTGTTGTGCCCAGAAATATTCCGAC
CCTGGAAGAACTAAAGCTCCCCCCTGTGATAATGAAGATAGCAGAAAAGCATCAAGGGTTGGTGTTGGTGACGGGCCCGA
CCGGAAGCGGAAAATCGACGACGCTTGCTTCGATGATTCATTATATGAATCAGACGATGAGAAAGCATATCATTACGCTT
GAGGATCCGATAGAGTACTTACATAAGCACGGTTCTTCCATTATTGATCAACGGGAAGTCGGGTTTGACACAAAGTCCTT
TGCATACGGATTGCGAAGTGCATTGAGGCAGGATCCCGATGTCATTCTGGTAGGAGAAATGCGCGATCTGGAAACCATTC
AAACAGCCATTACAGCAGCTGAAACCGGTCATTTAGTCCTGGGGACGCTGCATACGTCCAGTGCCGTTTCGACAATCGAA
CGGATTATAGATGTATTCCCGGCTGAACAGCAATCCCAGGTCCGGGTTCAGCTTGCAACGGTTCTGCAGGGAGTGATTGC
TCAGCGCCTCCTGCCTACAGTTGATAAAAAAGGCAGGATTGCTGTCTCGGAAATCCTCCTGAATAATTCAGCGGTTGCGA
ATCTTATCCGGTCTGAAAAGATTCATCAAATTCCCACCATCATGCAAACTTCTAAAGCGCAGGGAATGGAGCTCTTTGAA
TCGAATGTCAGCAGATATCTACACGCCGGTATGATTTCGAAAGAAGTGGCCTCTCCATTTCTTCAGGAGAACCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.149

97.101

0.487
  pilT Vibrio cholerae strain A1552

50.149

97.101

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.526

90.435

0.484
  pilT Neisseria meningitidis 8013

50

96.812

0.484
  pilT Neisseria gonorrhoeae MS11

49.401

96.812

0.478
  pilT Pseudomonas stutzeri DSM 10701

48.171

95.072

0.458
  pilT Legionella pneumophila strain Lp02

47.447

96.522

0.458
  pilT Legionella pneumophila strain ERS1305867

47.447

96.522

0.458
  pilT Pseudomonas aeruginosa PAK

46.951

95.072

0.446
  pilT Acinetobacter nosocomialis M2

45.646

96.522

0.441
  pilT Acinetobacter baumannii D1279779

45.646

96.522

0.441
  pilT Acinetobacter baumannii strain A118

45.646

96.522

0.441
  pilT Acinetobacter baylyi ADP1

45.897

95.362

0.438
  pilU Pseudomonas stutzeri DSM 10701

42.943

96.522

0.414
  pilU Vibrio cholerae strain A1552

42.547

93.333

0.397
  pilU Acinetobacter baylyi ADP1

40.063

91.884

0.368