Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V6667_RS09605 Genome accession   NZ_CP145811
Coordinates   2062971..2064011 (-) Length   346 a.a.
NCBI ID   WP_338809292.1    Uniprot ID   -
Organism   Neisseria leonii strain CCUG 45853     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2057971..2069011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6667_RS09570 (V6667_09570) - 2058230..2058505 (-) 276 WP_274585758.1 lipid asymmetry maintenance protein MlaB -
  V6667_RS09575 (V6667_09575) - 2058505..2059098 (-) 594 WP_338809283.1 ABC transporter substrate-binding protein -
  V6667_RS09580 (V6667_09580) mlaD 2059172..2059690 (-) 519 WP_338809285.1 outer membrane lipid asymmetry maintenance protein MlaD -
  V6667_RS09585 (V6667_09585) mlaE 2059696..2060472 (-) 777 WP_338809287.1 lipid asymmetry maintenance ABC transporter permease subunit MlaE -
  V6667_RS09590 (V6667_09590) - 2060476..2061303 (-) 828 WP_338809288.1 ABC transporter ATP-binding protein -
  V6667_RS09595 (V6667_09595) - 2061564..2061797 (-) 234 WP_274585763.1 cytochrome-c oxidase -
  V6667_RS09600 (V6667_09600) pdxA 2061832..2062827 (-) 996 WP_338809290.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
  V6667_RS09605 (V6667_09605) pilT 2062971..2064011 (-) 1041 WP_338809292.1 type IV pilus twitching motility protein PilT Machinery gene
  V6667_RS09610 (V6667_09610) - 2064103..2064795 (+) 693 WP_274585766.1 YggS family pyridoxal phosphate-dependent enzyme -
  V6667_RS09615 (V6667_09615) proC 2064988..2065803 (+) 816 WP_338809293.1 pyrroline-5-carboxylate reductase -
  V6667_RS09620 (V6667_09620) dksA 2065898..2066314 (-) 417 WP_274572504.1 RNA polymerase-binding protein DksA -
  V6667_RS09625 (V6667_09625) gap 2066626..2067633 (-) 1008 WP_338809294.1 type I glyceraldehyde-3-phosphate dehydrogenase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 36724.20 Da        Isoelectric Point: 7.0817

>NTDB_id=940028 V6667_RS09605 WP_338809292.1 2062971..2064011(-) (pilT) [Neisseria leonii strain CCUG 45853]
MADLVGFLLEAAQKGASDVHLAAGEPAVLRIDGSLTRTAQAVWTAEQIENAVLPLMNEAQRTVWRDGGEVDFAFVLPDNK
RCRANVFQTASGAAAALRLIADNIPTLAGLGVPPVVHKLAELPRGLVLVTGPTGSGKSTTLAAMIDALNRSRPLHILTIE
DPIEFVHTNRTALIRQRALHEHTKSFAAALKSALREDPDVILVGELRDRETVRLALTAAETGHLVLATLHTTGAAKTIDR
IIDVFEAGEKDSVRAMLSESLRAVVSQLLLPALGGGRVAAHEILMATPAVRHLIRENKTAQIANAIQTGQAAGMQTLAQS
LQQLLHSGRIDAQTAARFGGSESGLI

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=940028 V6667_RS09605 WP_338809292.1 2062971..2064011(-) (pilT) [Neisseria leonii strain CCUG 45853]
ATGGCGGACTTGGTCGGTTTTCTGTTGGAAGCGGCGCAAAAAGGTGCATCCGATGTCCATCTGGCGGCGGGCGAACCGGC
CGTTTTGCGTATCGACGGCAGCCTGACGCGTACCGCACAGGCGGTATGGACGGCAGAGCAAATCGAAAATGCCGTTTTGC
CGCTGATGAATGAGGCACAGCGTACCGTCTGGCGCGATGGCGGCGAAGTCGATTTTGCTTTTGTGCTGCCCGACAACAAA
CGGTGCCGTGCCAATGTTTTTCAGACGGCCTCCGGTGCGGCCGCCGCGTTGCGCCTGATTGCGGACAATATTCCGACATT
GGCCGGTTTGGGCGTACCGCCGGTGGTGCACAAGCTGGCCGAACTGCCGCGCGGTTTGGTGTTGGTAACCGGCCCCACCG
GCTCGGGCAAGTCCACCACGCTGGCGGCCATGATTGACGCGCTCAACCGCAGTCGGCCGCTGCATATTCTGACGATTGAA
GACCCGATCGAATTTGTCCATACCAACCGTACGGCATTAATCCGCCAGCGCGCGCTGCACGAGCACACCAAAAGTTTCGC
TGCCGCGCTCAAATCAGCGTTGCGCGAAGACCCCGATGTGATTCTGGTGGGCGAATTGCGCGACCGCGAAACCGTCCGCC
TGGCTTTAACGGCGGCCGAAACCGGCCATCTGGTGCTGGCCACGCTGCATACCACCGGCGCAGCGAAAACGATAGACCGC
ATTATCGATGTGTTTGAAGCGGGCGAAAAAGACAGCGTACGCGCCATGCTGTCCGAAAGCCTGCGTGCCGTGGTGTCGCA
GCTTTTGCTGCCCGCGCTCGGCGGCGGGCGCGTGGCGGCGCACGAAATTCTGATGGCGACACCGGCGGTGCGCCATTTAA
TCCGCGAGAACAAAACGGCGCAAATCGCCAATGCCATTCAAACCGGACAGGCCGCCGGTATGCAGACTTTGGCACAGTCA
TTGCAGCAGCTGCTGCACAGCGGCCGTATCGATGCGCAAACCGCCGCCCGTTTCGGCGGCAGCGAAAGCGGTTTGATATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

58.58

97.688

0.572
  pilT Neisseria meningitidis 8013

58.284

97.688

0.569
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

57.1

95.665

0.546
  pilT Vibrio cholerae strain A1552

57.1

95.665

0.546
  pilT Acinetobacter baylyi ADP1

56.456

96.243

0.543
  pilT Acinetobacter baumannii D1279779

55.856

96.243

0.538
  pilT Acinetobacter baumannii strain A118

55.856

96.243

0.538
  pilT Acinetobacter nosocomialis M2

55.856

96.243

0.538
  pilT Pseudomonas stutzeri DSM 10701

54.955

96.243

0.529
  pilT Legionella pneumophila strain Lp02

54.03

96.821

0.523
  pilT Legionella pneumophila strain ERS1305867

54.03

96.821

0.523
  pilT Pseudomonas aeruginosa PAK

54.354

96.243

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.538

93.931

0.465
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.532

0.39
  pilU Acinetobacter baylyi ADP1

37.391

99.711

0.373
  pilU Vibrio cholerae strain A1552

39.252

92.775

0.364