Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ORY85_RS07800 Genome accession   NZ_CP145606
Coordinates   1631385..1632425 (+) Length   346 a.a.
NCBI ID   WP_274572507.1    Uniprot ID   -
Organism   Neisseria leonii strain 3986     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1626385..1637425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORY85_RS07780 (ORY85_07780) gap 1627727..1628734 (+) 1008 WP_274572503.1 type I glyceraldehyde-3-phosphate dehydrogenase -
  ORY85_RS07785 (ORY85_07785) dksA 1629046..1629462 (+) 417 WP_274572504.1 RNA polymerase-binding protein DksA -
  ORY85_RS07790 (ORY85_07790) proC 1629557..1630372 (-) 816 WP_274572505.1 pyrroline-5-carboxylate reductase -
  ORY85_RS07795 (ORY85_07795) - 1630604..1631296 (-) 693 WP_274572506.1 YggS family pyridoxal phosphate-dependent enzyme -
  ORY85_RS07800 (ORY85_07800) pilT 1631385..1632425 (+) 1041 WP_274572507.1 type IV pilus twitching motility protein PilT Machinery gene
  ORY85_RS07805 (ORY85_07805) pdxA 1632568..1633563 (+) 996 WP_274572508.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
  ORY85_RS07810 (ORY85_07810) - 1633598..1633831 (+) 234 WP_274572509.1 cytochrome-c oxidase -
  ORY85_RS07815 (ORY85_07815) - 1634091..1634918 (+) 828 WP_274572510.1 ABC transporter ATP-binding protein -
  ORY85_RS07820 (ORY85_07820) mlaE 1634922..1635698 (+) 777 WP_274572511.1 lipid asymmetry maintenance ABC transporter permease subunit MlaE -
  ORY85_RS07825 (ORY85_07825) mlaD 1635704..1636222 (+) 519 WP_274572512.1 outer membrane lipid asymmetry maintenance protein MlaD -
  ORY85_RS07830 (ORY85_07830) - 1636296..1636889 (+) 594 WP_274572513.1 phospholipid-binding protein MlaC -
  ORY85_RS07835 (ORY85_07835) - 1636889..1637164 (+) 276 WP_274572514.1 lipid asymmetry maintenance protein MlaB -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 36812.22 Da        Isoelectric Point: 6.7283

>NTDB_id=939014 ORY85_RS07800 WP_274572507.1 1631385..1632425(+) (pilT) [Neisseria leonii strain 3986]
MADLVGFLLEAAQKGASDVHLAAGEPAVLRIDGSLTRTEQAAWTAEQIENAVLPLMNEAQRTVWRDGGEVDFAFVLPDNK
RCRANVFQTASGAAAALRLIADNIPTLADLGVPPVVHKLAELPRGLVLVTGPTGSGKSTTLAAMIDALNRSRPLHILTIE
DPIEFVHTNRTALIRQRALHEHTKSFAAALKSALREDPDVILVGELRDRETVRLALTAAETGHLVLATLHTTGAAKTIDR
IIDVFEAGEKDSVRAMLSESLRAVVSQLLLPALGGGRVAAHEILMATPAVRHLIRENKTAQIANAIQTGQAAGMQTLAQS
LQQLLHSGRIDAQTAARFGGSESGLI

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=939014 ORY85_RS07800 WP_274572507.1 1631385..1632425(+) (pilT) [Neisseria leonii strain 3986]
ATGGCGGACTTGGTCGGTTTTCTATTGGAAGCGGCGCAAAAAGGTGCATCCGATGTCCATCTGGCGGCGGGCGAACCGGC
CGTTTTGCGTATCGACGGCAGCCTGACGCGCACGGAACAGGCGGCATGGACGGCAGAGCAGATCGAAAATGCCGTTTTGC
CGCTGATGAACGAGGCACAGCGTACCGTCTGGCGCGATGGCGGCGAAGTCGATTTTGCGTTTGTGCTGCCCGACAACAAA
CGCTGCCGTGCCAATGTTTTTCAGACGGCCTCCGGTGCGGCCGCCGCGTTGCGCCTGATTGCGGACAATATTCCGACATT
GGCCGATTTGGGCGTACCGCCGGTGGTGCACAAGCTGGCTGAATTGCCGCGCGGTTTGGTGTTGGTCACCGGCCCCACCG
GTTCGGGCAAGTCCACCACGCTGGCAGCCATGATTGACGCGCTCAACCGCAGCCGGCCGCTGCATATTCTGACGATTGAA
GACCCGATCGAATTTGTCCATACCAACCGTACGGCATTAATCCGCCAGCGCGCGCTGCACGAGCACACCAAAAGTTTTGC
TGCCGCGCTCAAATCCGCGCTGCGCGAAGATCCCGATGTGATTCTGGTGGGCGAATTGCGTGACCGCGAAACCGTCCGGC
TGGCTTTAACGGCAGCCGAAACCGGACATTTGGTGCTGGCCACGCTGCATACCACCGGCGCGGCGAAAACGATAGACCGC
ATTATCGATGTGTTTGAAGCGGGCGAAAAAGACAGCGTACGCGCCATGCTGTCCGAAAGCCTGCGCGCCGTGGTGTCGCA
GCTTTTGCTGCCCGCGCTCGGCGGCGGGCGCGTGGCGGCGCACGAAATTCTGATGGCGACACCGGCGGTGCGCCATTTAA
TCCGCGAGAACAAAACGGCGCAAATCGCCAATGCCATTCAAACCGGACAGGCCGCCGGTATGCAGACTTTGGCACAGTCA
TTGCAGCAGCTGCTGCACAGCGGCCGTATCGATGCGCAAACCGCCGCCCGTTTCGGCGGCAGCGAAAGCGGCTTGATATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

58.58

97.688

0.572
  pilT Neisseria meningitidis 8013

58.284

97.688

0.569
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

57.402

95.665

0.549
  pilT Vibrio cholerae strain A1552

57.402

95.665

0.549
  pilT Acinetobacter baylyi ADP1

56.757

96.243

0.546
  pilT Acinetobacter baumannii D1279779

56.456

96.243

0.543
  pilT Acinetobacter baumannii strain A118

56.456

96.243

0.543
  pilT Acinetobacter nosocomialis M2

56.456

96.243

0.543
  pilT Pseudomonas stutzeri DSM 10701

55.556

96.243

0.535
  pilT Legionella pneumophila strain Lp02

54.328

96.821

0.526
  pilT Legionella pneumophila strain ERS1305867

54.328

96.821

0.526
  pilT Pseudomonas aeruginosa PAK

54.354

96.243

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.538

93.931

0.465
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

37.681

99.711

0.376
  pilU Vibrio cholerae strain A1552

39.252

92.775

0.364