Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V5G20_RS22160 Genome accession   NZ_CP144922
Coordinates   4626219..4627262 (-) Length   347 a.a.
NCBI ID   WP_003392164.1    Uniprot ID   M8E451
Organism   Brevibacillus borstelensis strain CP19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4621219..4632262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5G20_RS22135 (V5G20_22135) - 4621438..4622085 (-) 648 WP_338462242.1 PilN domain-containing protein -
  V5G20_RS22140 (V5G20_22140) pilM 4622091..4623119 (-) 1029 WP_003392157.1 pilus assembly protein PilM -
  V5G20_RS22145 (V5G20_22145) - 4623367..4624125 (-) 759 WP_024985515.1 A24 family peptidase -
  V5G20_RS22150 (V5G20_22150) - 4624530..4624979 (-) 450 WP_024985514.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  V5G20_RS22155 (V5G20_22155) - 4625010..4626218 (-) 1209 WP_003392162.1 type II secretion system F family protein -
  V5G20_RS22160 (V5G20_22160) pilT 4626219..4627262 (-) 1044 WP_003392164.1 type IV pilus twitching motility protein PilT Machinery gene
  V5G20_RS22165 (V5G20_22165) - 4627296..4628951 (-) 1656 WP_024985513.1 ATPase, T2SS/T4P/T4SS family -
  V5G20_RS22170 (V5G20_22170) - 4628957..4630621 (-) 1665 WP_338462247.1 VanW family protein -
  V5G20_RS22175 (V5G20_22175) - 4630630..4631823 (-) 1194 WP_327926165.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38420.39 Da        Isoelectric Point: 7.3933

>NTDB_id=935313 V5G20_RS22160 WP_003392164.1 4626219..4627262(-) (pilT) [Brevibacillus borstelensis strain CP19]
MAGVEIKKLLRYAFEKKASDLHVTVGTSPVFRIDGELRRLDISPLAPSDTEEMAKELIPQHLYPSFLEKGELDFSYGLPG
ISRFRVNAYHQRGCIGLVARVIPTGIPSLEELGLPEVIRKLCHKPQGLLLVTGPTGSGKSTTLAAMIDYMNRTMRKHIVT
LEDPIEYLHKHQLSMVNQREIGFDTKDFASGLRASLRQDPDVILVGEMRDLETISTAVTAAETGHLVLATLHTTDAPKTI
DRIIDVFPGDKQSQIRMQLSSVLVGIVSQRLFPKVGGGRAVATEILVHTHAVANLIRSEKNHQIKSLMQTGRELGMHTME
SSVKELVDKGQITPEAAQHYLNERALE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=935313 V5G20_RS22160 WP_003392164.1 4626219..4627262(-) (pilT) [Brevibacillus borstelensis strain CP19]
ATGGCTGGCGTCGAAATCAAAAAGCTGCTCAGATACGCTTTTGAAAAAAAGGCATCGGACCTGCATGTGACAGTAGGCAC
CTCTCCCGTATTCCGAATTGACGGGGAACTGCGCCGGCTCGACATTTCTCCGCTTGCGCCAAGCGACACCGAGGAAATGG
CCAAGGAGCTGATCCCGCAGCATTTGTATCCGTCCTTTCTGGAAAAGGGAGAATTGGATTTCTCCTACGGACTGCCGGGC
ATCTCGCGTTTCCGCGTCAATGCGTACCACCAGCGCGGGTGCATCGGGCTTGTCGCCCGGGTCATTCCGACAGGCATCCC
GTCACTGGAAGAGCTGGGACTGCCGGAGGTAATCCGGAAGCTTTGCCACAAGCCCCAAGGGTTACTGCTCGTTACAGGGC
CGACGGGAAGCGGCAAGTCGACTACATTGGCGGCGATGATCGACTACATGAACAGGACGATGAGAAAGCATATCGTTACG
CTGGAAGACCCGATCGAGTATTTGCACAAGCATCAGCTCTCCATGGTCAATCAGCGGGAGATCGGCTTTGATACCAAAGA
TTTTGCCAGCGGACTGCGGGCTTCGCTGCGGCAGGACCCTGACGTCATATTGGTCGGCGAGATGCGGGATCTGGAGACGA
TCTCGACAGCAGTCACAGCGGCGGAGACGGGACATTTGGTGCTCGCGACGCTGCATACAACCGATGCGCCCAAGACCATC
GACCGGATTATCGATGTCTTTCCGGGCGACAAGCAATCCCAGATTCGGATGCAGTTGTCTTCCGTTCTGGTCGGGATCGT
CTCGCAGCGCCTGTTTCCGAAGGTCGGGGGCGGCCGGGCAGTCGCCACTGAGATTCTCGTTCATACACATGCTGTCGCCA
ACCTGATACGCAGCGAGAAGAATCATCAAATCAAAAGCTTGATGCAGACGGGACGCGAACTGGGAATGCACACGATGGAA
AGCTCCGTCAAAGAGCTAGTGGACAAGGGACAGATTACTCCCGAAGCCGCGCAACACTATTTGAATGAAAGGGCGCTAGA
GTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M8E451

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.051

95.965

0.49
  pilT Vibrio cholerae strain A1552

50.595

96.83

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.595

96.83

0.49
  pilT Pseudomonas aeruginosa PAK

49.851

96.542

0.481
  pilT Pseudomonas stutzeri DSM 10701

49.851

96.542

0.481
  pilT Acinetobacter baylyi ADP1

48.955

96.542

0.473
  pilT Neisseria gonorrhoeae MS11

48.512

96.83

0.47
  pilT Neisseria meningitidis 8013

48.512

96.83

0.47
  pilT Acinetobacter baumannii strain A118

48.358

96.542

0.467
  pilT Acinetobacter baumannii D1279779

48.358

96.542

0.467
  pilT Acinetobacter nosocomialis M2

48.358

96.542

0.467
  pilT Legionella pneumophila strain Lp02

47.23

98.847

0.467
  pilT Legionella pneumophila strain ERS1305867

47.23

98.847

0.467
  pilU Pseudomonas stutzeri DSM 10701

44.776

96.542

0.432
  pilU Vibrio cholerae strain A1552

40.653

97.118

0.395
  pilU Acinetobacter baylyi ADP1

38.938

97.695

0.38