Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FTX54_RS12290 Genome accession   NZ_CP144914
Coordinates   2651801..2652817 (-) Length   338 a.a.
NCBI ID   WP_147803615.1    Uniprot ID   A0A5C7F7F7
Organism   Alkalicoccus halolimnae strain BZ-SZ-XJ29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2646801..2657817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FTX54_RS12275 (FTX54_012270) - 2647845..2648627 (-) 783 WP_147803502.1 A24 family peptidase -
  FTX54_RS12280 (FTX54_012275) - 2649692..2650165 (-) 474 WP_147803501.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FTX54_RS12285 (FTX54_012280) - 2650580..2651785 (-) 1206 WP_147803500.1 type II secretion system F family protein -
  FTX54_RS12290 (FTX54_012285) pilT 2651801..2652817 (-) 1017 WP_147803615.1 type IV pilus twitching motility protein PilT Machinery gene
  FTX54_RS12295 (FTX54_012290) - 2652852..2654507 (-) 1656 WP_147803499.1 GspE/PulE family protein -
  FTX54_RS12300 (FTX54_012295) - 2654525..2656519 (-) 1995 WP_338484769.1 VanW family protein -
  FTX54_RS12305 (FTX54_012300) - 2656531..2656818 (-) 288 WP_147803497.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37466.00 Da        Isoelectric Point: 6.3467

>NTDB_id=935119 FTX54_RS12290 WP_147803615.1 2651801..2652817(-) (pilT) [Alkalicoccus halolimnae strain BZ-SZ-XJ29]
MELLKEAFDKEASDIHITVGMPPVFRINGELVRRIGEAMKPEDTEAAARSVIPETMWDTFKEKGELDFSYGLPGVSRFRI
NAYYQRACISLAVRIVPTSVPTIQELGMPASLEKIAQKQQGLVLVTGPTGSGKSTTLASLIHYMNKTMNRHIITLEDPIE
YLHTHDKSLVDQREIGFDTHSFANGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVLGTLHTTDASSTVDRIIDV
FPPEQQGQVRIQTASVLAAVISQRLLLTADKQERVAATEILMNTPAVKNLIRNEKIHQIPTVMQTGRTQGMHTMEMSVRK
LLQGRRIDQETAAVYVSD

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=935119 FTX54_RS12290 WP_147803615.1 2651801..2652817(-) (pilT) [Alkalicoccus halolimnae strain BZ-SZ-XJ29]
ATGGAACTTTTAAAAGAAGCATTTGATAAAGAGGCGTCGGATATCCATATTACGGTCGGTATGCCTCCGGTTTTCCGCAT
TAACGGCGAACTCGTACGCCGCATCGGGGAAGCGATGAAGCCCGAAGATACTGAAGCAGCAGCCCGTAGCGTTATTCCGG
AAACGATGTGGGATACATTCAAAGAAAAAGGGGAGCTGGACTTTTCCTACGGACTTCCCGGTGTATCAAGATTCCGTATC
AACGCTTATTACCAGCGGGCGTGCATCAGCCTTGCTGTCCGAATTGTACCGACATCGGTCCCTACGATTCAGGAGCTCGG
CATGCCTGCTTCGCTCGAAAAAATAGCTCAGAAACAGCAGGGACTCGTGCTCGTGACCGGACCGACCGGATCGGGGAAAT
CAACGACGCTCGCTTCCCTGATCCACTACATGAACAAAACGATGAACCGTCATATTATTACGCTTGAGGATCCGATCGAG
TATCTGCATACTCACGATAAATCTCTCGTGGACCAGCGGGAAATCGGCTTTGATACACACAGCTTTGCCAATGGGCTGAG
AGCAGCTCTGCGGCAGGATCCCGATGTGATTCTCGTCGGGGAAATGCGCGATCTGGAGACGATCAGCACCGCAATTACCG
CAGCGGAAACCGGCCACCTTGTACTCGGTACGCTCCATACGACCGATGCCTCCTCTACGGTGGACCGCATCATCGACGTC
TTCCCTCCAGAACAGCAGGGCCAGGTCCGTATCCAGACGGCGTCGGTCCTCGCAGCTGTTATTTCCCAGCGTCTGCTGCT
CACCGCAGATAAACAGGAACGGGTCGCTGCAACCGAAATATTGATGAATACGCCGGCGGTGAAAAATTTGATCCGCAATG
AAAAAATCCACCAGATCCCTACCGTCATGCAGACTGGTCGCACACAGGGAATGCATACGATGGAAATGTCGGTGAGGAAG
CTGCTGCAGGGAAGAAGGATCGACCAGGAAACCGCGGCAGTTTACGTAAGTGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(2-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C7F7F7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.795

95.266

0.503
  pilT Vibrio cholerae strain A1552

50.45

98.521

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.45

98.521

0.497
  pilT Neisseria meningitidis 8013

49.849

97.929

0.488
  pilT Neisseria gonorrhoeae MS11

49.547

97.929

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.795

98.225

0.479
  pilT Acinetobacter baylyi ADP1

48.358

99.112

0.479
  pilT Acinetobacter baumannii D1279779

47.892

98.225

0.47
  pilT Acinetobacter nosocomialis M2

47.892

98.225

0.47
  pilT Acinetobacter baumannii strain A118

47.892

98.225

0.47
  pilT Pseudomonas aeruginosa PAK

47.59

98.225

0.467
  pilT Legionella pneumophila strain Lp02

45.619

97.929

0.447
  pilT Legionella pneumophila strain ERS1305867

45.619

97.929

0.447
  pilU Pseudomonas stutzeri DSM 10701

40.719

98.817

0.402
  pilU Vibrio cholerae strain A1552

38.82

95.266

0.37
  pilU Acinetobacter baylyi ADP1

37.309

96.746

0.361