Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V4D31_RS01805 Genome accession   NZ_CP144374
Coordinates   369059..370138 (+) Length   359 a.a.
NCBI ID   WP_353686542.1    Uniprot ID   A0AAU8H2I6
Organism   Thermodesulfovibrio sp. 3462-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 364059..375138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4D31_RS01785 (V4D31_01770) - 366642..366797 (+) 156 WP_353686538.1 hypothetical protein -
  V4D31_RS01790 (V4D31_01775) mazG 366856..367626 (-) 771 WP_353686539.1 nucleoside triphosphate pyrophosphohydrolase -
  V4D31_RS01795 (V4D31_01780) - 367619..368362 (-) 744 WP_353686540.1 AAA family ATPase -
  V4D31_RS01800 (V4D31_01785) - 368359..369000 (-) 642 WP_353686541.1 HAD-IA family hydrolase -
  V4D31_RS01805 (V4D31_01790) pilT 369059..370138 (+) 1080 WP_353686542.1 type IV pilus twitching motility protein PilT Machinery gene
  V4D31_RS01810 (V4D31_01795) - 370135..371307 (+) 1173 WP_353686543.1 PilT/PilU family type 4a pilus ATPase -
  V4D31_RS01815 (V4D31_01800) - 371313..371711 (+) 399 WP_353686544.1 hypothetical protein -
  V4D31_RS01820 (V4D31_01805) - 371762..372004 (+) 243 WP_353686545.1 Slp family lipoprotein -
  V4D31_RS01825 (V4D31_01810) mutL 372017..373621 (+) 1605 WP_353686546.1 DNA mismatch repair endonuclease MutL -
  V4D31_RS01830 (V4D31_01815) - 373769..374266 (+) 498 WP_353686547.1 flagellar basal body-associated protein FliL -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 40012.17 Da        Isoelectric Point: 6.8368

>NTDB_id=933874 V4D31_RS01805 WP_353686542.1 369059..370138(+) (pilT) [Thermodesulfovibrio sp. 3462-1]
MARIDAFFKLMLENKASDLHLVAGSQPVLRIHGELVRVQYKVLDNQELRSLLYEITPEEKIKVFEETGDLDFAHEIPGVA
RFRVNYFMHKEGIGAAFRHIPNEIKTIDQLGLPQVLKRFAMLKKGIVLVTGPTGSGKSTTLAAIIDYANTNRKERIITIE
DPIEFIHPNKGCVISYREVGTHTESFAKALRAALREDPDIILVGEMRDIETIQLALEAAATGHFVLSTLHTSSAIKTVDR
IIDVFPPEQQAQVRTSLSESLQAVVAQTLLRRADGRGRVAACEILINTYAVGNLIREGKTHQILSIMQTSKSLGMQTMDD
ALLQLLQEGKITAEDAYERATDKQKFARFLKEVPQEGIG

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=933874 V4D31_RS01805 WP_353686542.1 369059..370138(+) (pilT) [Thermodesulfovibrio sp. 3462-1]
ATGGCTCGCATTGATGCTTTTTTCAAATTAATGCTTGAAAACAAAGCAAGTGACCTTCACCTTGTTGCTGGAAGCCAGCC
TGTATTAAGAATTCATGGAGAACTTGTGAGGGTTCAGTATAAGGTTCTTGATAATCAGGAACTGAGGTCTCTTCTTTATG
AAATCACACCAGAGGAAAAAATCAAAGTATTTGAAGAAACAGGTGATCTTGATTTTGCTCATGAAATCCCTGGTGTTGCT
CGTTTTCGTGTGAACTACTTCATGCATAAAGAAGGAATTGGTGCAGCCTTCAGGCATATTCCCAATGAAATAAAAACAAT
TGATCAACTTGGTTTGCCACAGGTGCTAAAGCGCTTTGCAATGCTTAAGAAAGGAATTGTTCTTGTTACAGGACCTACAG
GTTCTGGAAAATCAACAACCTTGGCTGCAATAATTGATTATGCAAATACTAATAGAAAAGAAAGAATAATCACCATTGAA
GACCCAATTGAATTTATTCATCCCAACAAAGGATGTGTCATATCTTACAGAGAAGTGGGAACTCATACAGAGTCCTTTGC
AAAAGCTCTGAGAGCAGCACTTAGAGAAGACCCTGATATAATTCTTGTGGGTGAAATGCGTGATATTGAGACAATTCAGC
TTGCCCTTGAGGCTGCTGCAACTGGTCATTTTGTTCTGAGCACCCTTCATACATCATCGGCAATAAAAACAGTTGATAGA
ATTATTGATGTCTTCCCTCCTGAGCAGCAGGCACAGGTAAGAACATCACTGAGTGAGTCTTTACAGGCAGTTGTTGCTCA
AACTCTTTTAAGAAGAGCTGATGGCCGTGGAAGAGTCGCAGCCTGTGAGATTTTGATTAATACCTATGCTGTTGGAAATC
TTATAAGAGAGGGAAAAACTCACCAGATACTTTCAATTATGCAGACAAGTAAAAGCCTAGGTATGCAGACAATGGACGAT
GCTTTACTACAACTTCTTCAGGAAGGTAAAATCACTGCAGAAGATGCCTATGAAAGGGCAACAGACAAACAAAAGTTTGC
AAGATTTCTTAAGGAAGTTCCTCAGGAAGGTATTGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.381

92.201

0.501
  pilT Acinetobacter baylyi ADP1

52.478

95.543

0.501
  pilT Acinetobacter baumannii D1279779

52.187

95.543

0.499
  pilT Acinetobacter baumannii strain A118

52.187

95.543

0.499
  pilT Acinetobacter nosocomialis M2

52.187

95.543

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.281

99.164

0.499
  pilT Pseudomonas stutzeri DSM 10701

53.776

92.201

0.496
  pilT Legionella pneumophila strain ERS1305867

52.836

93.315

0.493
  pilT Legionella pneumophila strain Lp02

52.836

93.315

0.493
  pilT Neisseria meningitidis 8013

51.045

93.315

0.476
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.893

93.593

0.476
  pilT Vibrio cholerae strain A1552

50.893

93.593

0.476
  pilT Neisseria gonorrhoeae MS11

50.746

93.315

0.474
  pilU Pseudomonas stutzeri DSM 10701

42.241

96.936

0.409
  pilU Acinetobacter baylyi ADP1

42.985

93.315

0.401
  pilU Vibrio cholerae strain A1552

40.805

96.936

0.396