Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MWM02_RS04555 Genome accession   NZ_AP025627
Coordinates   903479..904537 (+) Length   352 a.a.
NCBI ID   WP_064549675.1    Uniprot ID   A0A1B7KWL2
Organism   Parageobacillus sp. KH3-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 898479..909537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM02_RS04540 (PspKH34_08530) - 899610..900410 (+) 801 WP_244403038.1 PRC-barrel domain-containing protein -
  MWM02_RS04545 (PspKH34_08540) - 900431..901792 (+) 1362 WP_244403039.1 VanW family protein -
  MWM02_RS04550 (PspKH34_08550) - 901796..903466 (+) 1671 WP_244403040.1 ATPase, T2SS/T4P/T4SS family -
  MWM02_RS04555 (PspKH34_08560) pilT 903479..904537 (+) 1059 WP_064549675.1 type IV pilus twitching motility protein PilT Machinery gene
  MWM02_RS04560 (PspKH34_08570) - 904524..905735 (+) 1212 WP_244403041.1 type II secretion system F family protein -
  MWM02_RS04565 (PspKH34_08580) - 905829..906353 (+) 525 WP_244403042.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MWM02_RS04570 (PspKH34_08590) - 906368..907117 (+) 750 WP_244403043.1 A24 family peptidase -
  MWM02_RS04575 (PspKH34_08600) pilM 907132..908094 (+) 963 WP_244403044.1 pilus assembly protein PilM -
  MWM02_RS04580 (PspKH34_08610) - 908075..908677 (+) 603 WP_244403045.1 fimbrial protein -
  MWM02_RS04585 (PspKH34_08620) pilO 908674..909381 (+) 708 WP_064549679.1 type 4a pilus biogenesis protein PilO -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38915.13 Da        Isoelectric Point: 9.2635

>NTDB_id=93282 MWM02_RS04555 WP_064549675.1 903479..904537(+) (pilT) [Parageobacillus sp. KH3-4]
MRQRLDALLRAAFEVKASDVHLTVGVPPIFRINGELKKYGQDVLRPEDTEGMAKAIIPSHLWLRFQEKGELDFSYGIPGV
SRFRVNTYRQRSCVSLAIRIIPTKIPTIDELRLPEVIKQIAQKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMRKHIITL
EDPIEYLHKHGSCIIDQREVGFDTNNFANGLRAALRQDPDVILVGELRDLETIHTAITAAETGHLVLGTLHTSSAPATID
RIIDVFPPAQQSQIRIQLASVLVAIISQRLFPTPQKTGRIAATEILINNAAVANLIRNEKVHQIVNVMQTSRALGMRTLA
MSIKELMQAGLILRDAAEPYLAQESGDSYASV

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=93282 MWM02_RS04555 WP_064549675.1 903479..904537(+) (pilT) [Parageobacillus sp. KH3-4]
ATGAGACAAAGATTGGACGCTCTTTTACGTGCAGCGTTTGAAGTAAAAGCCTCCGACGTTCATTTAACGGTCGGCGTGCC
GCCGATTTTCCGCATCAATGGCGAATTAAAAAAATACGGCCAAGACGTTTTGCGGCCGGAAGATACAGAAGGAATGGCCA
AGGCGATCATCCCGTCTCATTTATGGCTGCGATTTCAGGAAAAAGGAGAATTGGATTTTTCCTACGGGATTCCCGGCGTC
TCCCGCTTTCGCGTCAATACGTATAGGCAGCGGTCGTGCGTGTCGCTGGCCATCCGCATCATTCCGACAAAAATCCCAAC
GATTGATGAACTGCGCCTTCCTGAAGTGATAAAGCAAATCGCGCAAAAGCCGCACGGGCTTGTGCTAGTGACAGGACCGA
CGGGAAGCGGAAAGTCGACGACGCTTGCGGCAATGATCGACTATATGAACAAAACGATGCGCAAACATATTATTACGCTG
GAGGATCCAATTGAATATTTGCACAAGCACGGAAGCTGCATTATTGACCAGCGTGAAGTCGGCTTTGATACGAACAATTT
TGCCAACGGGTTGCGTGCGGCACTAAGGCAAGATCCCGATGTCATTTTAGTCGGGGAACTGCGCGATTTGGAAACGATCC
ATACCGCGATTACCGCCGCGGAAACGGGGCATCTCGTCCTTGGCACGCTACATACATCGAGCGCGCCGGCAACGATCGAC
CGGATTATTGACGTGTTTCCGCCTGCCCAGCAGTCACAAATCCGCATTCAGCTGGCGTCTGTTCTTGTCGCGATTATTTC
TCAACGGCTCTTTCCAACGCCGCAAAAAACAGGGCGGATCGCCGCCACGGAAATTTTAATCAATAACGCTGCGGTCGCCA
ATTTAATACGCAACGAAAAAGTTCATCAAATCGTCAACGTCATGCAAACAAGCCGCGCCCTTGGCATGCGTACGCTGGCG
ATGAGCATTAAAGAGCTGATGCAAGCAGGGCTGATCCTTCGCGACGCGGCTGAACCTTATCTTGCGCAGGAGAGTGGCGA
TTCGTATGCCTCAGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B7KWL2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.439

93.182

0.489
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.755

94.034

0.477
  pilT Vibrio cholerae strain A1552

50.755

94.034

0.477
  pilT Pseudomonas stutzeri DSM 10701

49.102

94.886

0.466
  pilT Pseudomonas aeruginosa PAK

49.695

93.182

0.463
  pilT Acinetobacter baumannii D1279779

49.845

91.761

0.457
  pilT Acinetobacter nosocomialis M2

49.845

91.761

0.457
  pilT Acinetobacter baumannii strain A118

49.845

91.761

0.457
  pilT Neisseria meningitidis 8013

47.879

93.75

0.449
  pilT Neisseria gonorrhoeae MS11

47.879

93.75

0.449
  pilT Acinetobacter baylyi ADP1

48.308

92.33

0.446
  pilT Legionella pneumophila strain ERS1305867

46.97

93.75

0.44
  pilT Legionella pneumophila strain Lp02

46.97

93.75

0.44
  pilU Pseudomonas stutzeri DSM 10701

43.544

94.602

0.412
  pilU Vibrio cholerae strain A1552

39.058

100

0.401
  pilU Acinetobacter baylyi ADP1

40.867

91.761

0.375

Multiple sequence alignment