Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VZH09_RS06385 Genome accession   NZ_CP143859
Coordinates   1330667..1331755 (-) Length   362 a.a.
NCBI ID   WP_338462947.1    Uniprot ID   -
Organism   Synechococcus elongatus IITB7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1325667..1336755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VZH09_RS06365 (VZH09_06365) dusA 1326597..1327610 (-) 1014 WP_338463023.1 tRNA dihydrouridine(20/20a) synthase DusA -
  VZH09_RS06370 (VZH09_06370) - 1327637..1328869 (+) 1233 WP_338462944.1 NAD(P)/FAD-dependent oxidoreductase -
  VZH09_RS06375 (VZH09_06375) - 1328849..1329406 (-) 558 WP_338462945.1 hypothetical protein -
  VZH09_RS06380 (VZH09_06380) - 1329411..1330643 (-) 1233 WP_338462946.1 type II secretion system F family protein -
  VZH09_RS06385 (VZH09_06385) pilT 1330667..1331755 (-) 1089 WP_338462947.1 type IV pilus twitching motility protein PilT Machinery gene
  VZH09_RS06390 (VZH09_06390) pilB/pilB1 1331827..1333827 (-) 2001 WP_338462948.1 GspE/PulE family protein Machinery gene
  VZH09_RS06395 (VZH09_06395) grpE 1334113..1334736 (+) 624 WP_338462949.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 39867.98 Da        Isoelectric Point: 6.3643

>NTDB_id=931580 VZH09_RS06385 WP_338462947.1 1330667..1331755(-) (pilT) [Synechococcus elongatus IITB7]
MELMIEDLMEKLVEMGGSDLHLSVGLPPYFRISGKLTPIGDEPMGEEQCQRLIFSMLNNTQRKMLEQNWELDCSYGIRGL
ARFRVNVYKDKGTYAACLRALSAKVPEFNDLGLPQVVREMSEKPRGLILVTGPTGSGKTTTLAAIIELINRSRAEHIITV
EDPIEFVYQPAKSIIHQRQLGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RIIDVFPAEKQTQVRVQLSNSLVAVFSQTLVPKRNARPGSFGRVLAQEIMIVTPAVSNLIREGKTAQIYSQIQTGGKLGM
QTLEKALADLYKAGEITFEAAASKTSRPDEMQRLIGNVSIAA

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=931580 VZH09_RS06385 WP_338462947.1 1330667..1331755(-) (pilT) [Synechococcus elongatus IITB7]
ATGGAACTAATGATCGAAGACCTGATGGAAAAGCTCGTGGAGATGGGAGGATCTGACCTCCATCTTTCTGTTGGCTTACC
TCCTTATTTTCGAATCAGTGGAAAACTTACCCCTATCGGGGATGAACCAATGGGTGAGGAACAGTGTCAGCGCTTGATCT
TCAGTATGTTGAACAACACGCAACGCAAAATGCTGGAGCAAAATTGGGAGCTTGATTGTTCCTACGGGATTCGTGGACTC
GCTCGTTTCCGAGTCAATGTTTACAAAGATAAGGGAACCTATGCCGCTTGCTTACGGGCTTTGAGTGCCAAGGTGCCGGA
GTTTAACGACCTAGGGCTACCTCAAGTGGTGCGCGAGATGAGCGAAAAGCCAAGGGGGCTCATTCTCGTCACTGGCCCTA
CAGGGTCTGGAAAAACAACAACGTTGGCAGCCATTATTGAGTTAATTAACCGCAGTCGAGCTGAACATATCATTACGGTT
GAAGACCCCATTGAATTTGTCTATCAACCAGCAAAAAGCATCATTCACCAGCGACAGCTTGGAGAAGATACGAAGAGTTT
TGCTAATGCCCTGCGAGCGGCGCTTCGAGAAGATCCTGACATCATTCTTGTGGGTGAGATGCGGGACTTGGAAACGATTC
AGTTAGCTATCACTGCTGCAGAAACTGGTCACCTCGTTTTTGGTACTTTGCACACCAGCTCCGCTGCTCAAACCGTTGAC
CGGATTATCGATGTCTTTCCTGCTGAAAAACAGACGCAGGTAAGGGTTCAGTTGTCGAATTCACTAGTTGCGGTCTTCAG
TCAAACCCTTGTTCCCAAACGCAACGCTCGACCTGGTAGCTTTGGTCGTGTCCTTGCCCAAGAGATCATGATTGTTACAC
CAGCAGTTTCCAACTTAATTCGCGAAGGAAAAACCGCACAAATTTACTCTCAAATTCAGACGGGCGGAAAACTAGGCATG
CAGACCCTAGAGAAAGCTTTGGCTGATCTTTATAAGGCAGGTGAAATCACTTTTGAAGCAGCTGCCTCTAAAACATCTAG
GCCCGATGAAATGCAACGGTTGATTGGCAATGTTTCAATTGCTGCCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(48-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.026

95.856

0.508
  pilT Pseudomonas aeruginosa PAK

52.738

95.856

0.506
  pilT Legionella pneumophila strain ERS1305867

53.043

95.304

0.506
  pilT Acinetobacter baylyi ADP1

53.043

95.304

0.506
  pilT Legionella pneumophila strain Lp02

53.043

95.304

0.506
  pilT Acinetobacter baumannii strain A118

52.174

95.304

0.497
  pilT Acinetobacter baumannii D1279779

52.174

95.304

0.497
  pilT Acinetobacter nosocomialis M2

52.174

95.304

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.296

98.066

0.483
  pilT Neisseria meningitidis 8013

50.143

96.409

0.483
  pilT Neisseria gonorrhoeae MS11

50.143

96.409

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.118

93.923

0.461
  pilT Vibrio cholerae strain A1552

49.118

93.923

0.461
  pilU Pseudomonas stutzeri DSM 10701

39.886

96.961

0.387
  pilU Acinetobacter baylyi ADP1

37.536

96.409

0.362