Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VZG28_RS07045 Genome accession   NZ_CP143853
Coordinates   1437043..1438131 (-) Length   362 a.a.
NCBI ID   WP_338431711.1    Uniprot ID   -
Organism   Synechococcus elongatus IITB4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1432043..1443131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VZG28_RS07025 (VZG28_07025) dusA 1432972..1433985 (-) 1014 WP_208676897.1 tRNA dihydrouridine(20/20a) synthase DusA -
  VZG28_RS07030 (VZG28_07030) - 1434012..1435244 (+) 1233 WP_338442691.1 NAD(P)/FAD-dependent oxidoreductase -
  VZG28_RS07035 (VZG28_07035) - 1435224..1435781 (-) 558 WP_338442692.1 hypothetical protein -
  VZG28_RS07040 (VZG28_07040) - 1435786..1437018 (-) 1233 WP_338442694.1 type II secretion system F family protein -
  VZG28_RS07045 (VZG28_07045) pilT 1437043..1438131 (-) 1089 WP_338431711.1 type IV pilus twitching motility protein PilT Machinery gene
  VZG28_RS07050 (VZG28_07050) pilB/pilB1 1438203..1440203 (-) 2001 WP_338442696.1 GspE/PulE family protein Machinery gene
  VZG28_RS07055 (VZG28_07055) grpE 1440489..1441112 (+) 624 WP_338442697.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 39837.99 Da        Isoelectric Point: 6.3643

>NTDB_id=931569 VZG28_RS07045 WP_338431711.1 1437043..1438131(-) (pilT) [Synechococcus elongatus IITB4]
MELMIEDLMEKLVEMGGSDLHLSVGLPPYFRISGKLTPIGDEPMGEEQCQRLIFSMLNNTQRKMLEQNWELDCSYGIRGL
ARFRVNVYKDKGTYAACLRALSAKVPEFNDLGLPQVVREMSEKPRGLILVTGPTGSGKTTTLAAVIELINRSRAEHIITV
EDPIEFVYQPAKSIIHQRQLGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RIIDVFPAEKQTQVRVQLSNSLVAVFSQTLVPKRNARPGSFGRLLAQEIMIVTPAISNLIREGKTAQIYSQIQTGGKLGM
QTLEKALADLYKAGEITFEAAAAKTSKPDEMQRLIGNVSIAA

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=931569 VZG28_RS07045 WP_338431711.1 1437043..1438131(-) (pilT) [Synechococcus elongatus IITB4]
ATGGAACTAATGATCGAAGACCTGATGGAAAAGCTCGTGGAGATGGGAGGATCTGACCTCCATCTTTCTGTTGGCTTACC
TCCTTATTTTCGAATCAGTGGAAAACTTACCCCCATCGGGGATGAACCAATGGGTGAGGAACAGTGTCAGCGCTTGATCT
TCAGTATGTTGAACAACACGCAACGCAAAATGCTGGAGCAAAATTGGGAGCTTGATTGTTCCTACGGGATTCGTGGACTC
GCTCGTTTCCGGGTCAATGTTTACAAAGATAAGGGAACCTATGCTGCTTGCTTACGGGCTTTGAGTGCTAAGGTGCCGGA
GTTTAACGACCTAGGGCTACCTCAAGTGGTGCGCGAGATGAGTGAAAAACCAAGGGGGCTCATTCTAGTCACTGGCCCTA
CAGGGTCTGGAAAAACAACAACGTTGGCAGCCGTTATTGAGTTAATTAACCGCAGCAGAGCTGAACATATCATTACGGTT
GAAGATCCCATTGAGTTTGTCTATCAACCTGCAAAAAGCATCATTCATCAACGACAGCTTGGAGAAGATACGAAGAGTTT
TGCTAATGCCCTGCGAGCAGCGCTTCGAGAAGATCCTGACATCATTCTTGTAGGTGAGATGCGGGACTTGGAAACGATTC
AGTTAGCTATCACTGCTGCAGAAACTGGTCACCTCGTTTTTGGCACCTTGCACACCAGCTCCGCTGCTCAAACTGTTGAC
CGGATTATCGATGTCTTCCCTGCTGAAAAACAGACGCAGGTAAGGGTTCAGTTGTCGAATTCACTAGTTGCGGTCTTCAG
TCAAACCCTTGTCCCCAAACGCAACGCTCGACCTGGAAGCTTTGGTCGGCTTCTAGCTCAGGAAATTATGATTGTTACGC
CGGCAATATCCAACCTGATTCGCGAGGGTAAAACAGCACAAATCTACTCTCAGATTCAGACGGGAGGAAAGCTAGGGATG
CAGACCCTAGAGAAAGCTTTAGCCGATCTTTACAAAGCAGGAGAAATCACTTTTGAAGCAGCGGCAGCCAAGACATCTAA
ACCAGATGAAATGCAGCGCCTGATCGGTAATGTTTCGATTGCTGCCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(48-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.026

95.856

0.508
  pilT Acinetobacter baylyi ADP1

53.333

95.304

0.508
  pilT Pseudomonas aeruginosa PAK

52.738

95.856

0.506
  pilT Legionella pneumophila strain ERS1305867

53.709

93.094

0.5
  pilT Legionella pneumophila strain Lp02

53.709

93.094

0.5
  pilT Acinetobacter baumannii strain A118

52.174

95.304

0.497
  pilT Acinetobacter nosocomialis M2

52.174

95.304

0.497
  pilT Acinetobacter baumannii D1279779

52.174

95.304

0.497
  pilT Neisseria gonorrhoeae MS11

49.57

96.409

0.478
  pilT Neisseria meningitidis 8013

49.57

96.409

0.478
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.732

98.066

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.824

93.923

0.459
  pilT Vibrio cholerae strain A1552

48.824

93.923

0.459
  pilU Pseudomonas stutzeri DSM 10701

39.886

96.961

0.387
  pilU Acinetobacter baylyi ADP1

37.536

96.409

0.362