Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P9935_RS02405 Genome accession   NZ_CP143648
Coordinates   511586..512620 (+) Length   344 a.a.
NCBI ID   WP_285347043.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CPO459     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506586..517620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P9935_RS02380 (P9935_002380) - 506874..508841 (-) 1968 WP_003121751.1 dynamin-like GTPase family protein -
  P9935_RS02385 (P9935_002385) - 508949..509245 (-) 297 WP_031628111.1 DUF167 domain-containing protein -
  P9935_RS02390 (P9935_002390) - 509242..509835 (-) 594 WP_003084544.1 YggT family protein -
  P9935_RS02395 (P9935_002395) proC 509846..510667 (-) 822 WP_003084546.1 pyrroline-5-carboxylate reductase -
  P9935_RS02400 (P9935_002400) - 510679..511371 (-) 693 WP_015649764.1 YggS family pyridoxal phosphate-dependent enzyme -
  P9935_RS02405 (P9935_002405) pilT 511586..512620 (+) 1035 WP_285347043.1 type IV pilus twitching motility protein PilT Machinery gene
  P9935_RS02410 (P9935_002410) pilU 512798..513946 (+) 1149 WP_003100959.1 type IV pilus ATPase PilU Machinery gene
  P9935_RS02415 (P9935_002415) czcD 513954..514853 (-) 900 WP_003100957.1 CDF family zinc transporter CzcD -
  P9935_RS02420 (P9935_002420) - 515007..515411 (+) 405 WP_003084560.1 NINE protein -
  P9935_RS02425 (P9935_002425) - 515654..517027 (+) 1374 WP_003100955.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38010.85 Da        Isoelectric Point: 6.8364

>NTDB_id=930323 P9935_RS02405 WP_285347043.1 511586..512620(+) (pilT) [Pseudomonas aeruginosa strain CPO459]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGEVFKRVSDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDSDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQSVISQTLIKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLVAKGLISRENAREKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=930323 P9935_RS02405 WP_285347043.1 511586..512620(+) (pilT) [Pseudomonas aeruginosa strain CPO459]
ATGGATATTACCGAGCTGCTCGCCTTCAGTGCCAAACAGGGCGCTTCGGACCTGCACCTCTCCGCCGGCCTGCCACCCAT
GATCCGGGTGGATGGCGATGTACGCCGGATCAACCTGCCACCGCTGGAACACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAATTCCTCGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTATTCCGGACCATTCCCTCCAAGGTACTGACCATGGA
GGAGCTTGGCATGGGAGAAGTGTTCAAACGTGTTTCAGACGTCCCGCGCGGGTTGGTACTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCGATGCTCGATTACCTGAATAACACCAAGTACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCTCGGCTTCAGCGA
AGCGCTGCGCTCGGCGCTGCGGGAGGACTCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CCCTGACCGCGGCGGAGACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCGAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAAAAGGCCATGGTTCGCTCGATGCTCTCCGAGTCGCTGCAATCGGTGATCTCGCAGAC
CCTGATCAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTCGACATGTGCCTC
AAGGGCCTGGTCGCCAAGGGCCTGATCAGCCGCGAGAACGCCCGCGAGAAGGCGAAGATCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

99.709

100

0.997
  pilT Pseudomonas stutzeri DSM 10701

93.895

100

0.939
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

81.395

100

0.814
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.471

98.837

0.657
  pilT Vibrio cholerae strain A1552

66.471

98.837

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392