Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1502_RS15445 Genome accession   NZ_CP143546
Coordinates   3114429..3115472 (-) Length   347 a.a.
NCBI ID   WP_409251524.1    Uniprot ID   -
Organism   Bacillus sp. SCS-153A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3109429..3120472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1502_RS15415 (V1502_15415) - 3109558..3110319 (-) 762 WP_409251518.1 hypothetical protein -
  V1502_RS15420 (V1502_15420) - 3110319..3110933 (-) 615 WP_409251519.1 PilN domain-containing protein -
  V1502_RS15425 (V1502_15425) pilM 3110933..3111895 (-) 963 WP_409251520.1 type IV pilus biogenesis protein PilM -
  V1502_RS15430 (V1502_15430) - 3111914..3112666 (-) 753 WP_409251521.1 prepilin peptidase -
  V1502_RS15435 (V1502_15435) - 3112666..3113106 (-) 441 WP_409251522.1 type II secretion system protein -
  V1502_RS15440 (V1502_15440) - 3113218..3114429 (-) 1212 WP_409251523.1 type II secretion system F family protein -
  V1502_RS15445 (V1502_15445) pilT 3114429..3115472 (-) 1044 WP_409251524.1 type IV pilus twitching motility protein PilT Machinery gene
  V1502_RS15450 (V1502_15450) - 3115482..3117143 (-) 1662 WP_409251525.1 GspE/PulE family protein -
  V1502_RS15455 (V1502_15455) - 3117171..3118535 (-) 1365 WP_409251526.1 VanW family protein -
  V1502_RS15460 (V1502_15460) - 3118555..3119292 (-) 738 WP_409251527.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38597.65 Da        Isoelectric Point: 8.1494

>NTDB_id=929571 V1502_RS15445 WP_409251524.1 3114429..3115472(-) (pilT) [Bacillus sp. SCS-153A]
MKERIDYLLRSAYELKASDIHLTVGAPPVFRLHGDLRRYGKEVLKPADTEGMAKSIIPEDLWKMFLDQGELDFSYGIPGI
SRFRINAYQQRSCVSLAIRIVPTSIPSVDELGLPPVLKKITEKPQGLVLVTGPTGSGKSTTLASMIDHINRTMRKHVITL
EDPIEYLHKHGTSIIDQREVGFDTKNFSKGLRSALRQDPDIILVGEMRDLETIHTAITAAETGHLVFATLHTSSAAATIE
RIVDVFPPEQQAQIRIQLASVLVAILSQRLFPVVDKRGRRAATELLVNNAAIANLIRSEKVHQIDNILQTSKGLGMHLMS
TSIKNLYESGVISRESAFPFIEMEQMS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=929571 V1502_RS15445 WP_409251524.1 3114429..3115472(-) (pilT) [Bacillus sp. SCS-153A]
ATGAAAGAAAGAATAGATTACTTATTGCGTTCAGCGTATGAATTGAAGGCATCTGATATACACTTGACAGTCGGGGCTCC
TCCCGTTTTTCGGCTTCATGGTGACCTGAGGCGATACGGAAAGGAAGTATTAAAACCGGCTGATACGGAAGGTATGGCAA
AGTCGATCATCCCTGAAGATCTTTGGAAAATGTTTTTAGATCAGGGAGAGCTTGATTTTTCTTACGGTATCCCTGGCATT
TCACGGTTCAGGATCAATGCTTATCAGCAACGCTCCTGTGTCTCGCTTGCCATCCGGATTGTTCCGACAAGCATACCGAG
TGTTGATGAGCTTGGTCTTCCGCCTGTATTAAAGAAAATCACGGAAAAACCTCAAGGTCTTGTGTTGGTGACAGGTCCTA
CAGGAAGCGGAAAATCGACTACACTCGCGTCAATGATCGATCATATTAACCGTACGATGAGGAAGCATGTCATTACACTG
GAAGATCCCATTGAGTATCTTCATAAACATGGAACCAGCATCATCGATCAGCGTGAAGTCGGATTCGACACAAAGAACTT
TTCTAAAGGTCTTCGAAGCGCTCTCCGTCAGGATCCGGACATTATTCTTGTCGGCGAGATGCGGGACCTGGAAACAATCC
ATACAGCGATCACCGCGGCGGAGACGGGCCACCTCGTGTTCGCCACACTTCATACATCAAGTGCGGCAGCGACCATTGAA
CGGATTGTGGATGTATTTCCTCCTGAACAGCAGGCTCAGATAAGAATACAGCTTGCCTCTGTCCTGGTAGCGATCCTCTC
ACAAAGACTATTTCCTGTTGTGGATAAAAGGGGAAGAAGAGCTGCAACAGAATTACTTGTCAACAATGCGGCCATCGCCA
ATTTGATCCGGTCTGAAAAGGTCCATCAAATCGATAATATTTTACAGACATCCAAAGGGCTGGGCATGCACCTCATGTCT
ACCAGCATTAAGAATCTCTATGAATCCGGGGTCATTTCCCGTGAATCCGCCTTCCCTTTTATTGAAATGGAGCAGATGTC
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(95-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.898

96.254

0.49
  pilT Pseudomonas stutzeri DSM 10701

48.649

95.965

0.467
  pilT Pseudomonas aeruginosa PAK

48.348

95.965

0.464
  pilT Neisseria meningitidis 8013

46.707

96.254

0.45
  pilT Acinetobacter nosocomialis M2

46.547

95.965

0.447
  pilT Acinetobacter baumannii D1279779

46.547

95.965

0.447
  pilT Acinetobacter baumannii strain A118

46.547

95.965

0.447
  pilT Neisseria gonorrhoeae MS11

46.407

96.254

0.447
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45

97.983

0.441
  pilT Vibrio cholerae strain A1552

45

97.983

0.441
  pilT Legionella pneumophila strain Lp02

45.646

95.965

0.438
  pilT Legionella pneumophila strain ERS1305867

45.646

95.965

0.438
  pilT Acinetobacter baylyi ADP1

45.646

95.965

0.438
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.254

0.386
  pilU Acinetobacter baylyi ADP1

39.759

95.677

0.38
  pilU Vibrio cholerae strain A1552

39.319

93.084

0.366