Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1501_RS15625 Genome accession   NZ_CP143545
Coordinates   3082165..3083217 (-) Length   350 a.a.
NCBI ID   WP_409289547.1    Uniprot ID   -
Organism   Peribacillus sp. SCS-37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3077165..3088217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1501_RS15595 (V1501_15595) - 3077202..3077930 (-) 729 WP_409289537.1 pilus assembly protein PilO -
  V1501_RS15600 (V1501_15600) - 3077927..3078604 (-) 678 WP_409289539.1 PilN domain-containing protein -
  V1501_RS15605 (V1501_15605) pilM 3078607..3079599 (-) 993 WP_409289540.1 type IV pilus biogenesis protein PilM -
  V1501_RS15610 (V1501_15610) - 3079624..3080379 (-) 756 WP_409289542.1 prepilin peptidase -
  V1501_RS15615 (V1501_15615) - 3080380..3080853 (-) 474 WP_409289543.1 type II secretion system protein -
  V1501_RS15620 (V1501_15620) - 3080967..3082178 (-) 1212 WP_409289545.1 type II secretion system F family protein -
  V1501_RS15625 (V1501_15625) pilT 3082165..3083217 (-) 1053 WP_409289547.1 type IV pilus twitching motility protein PilT Machinery gene
  V1501_RS15630 (V1501_15630) - 3083229..3084890 (-) 1662 WP_409293400.1 GspE/PulE family protein -
  V1501_RS15635 (V1501_15635) - 3084919..3086346 (-) 1428 WP_409289548.1 hypothetical protein -
  V1501_RS15640 (V1501_15640) - 3086350..3087072 (-) 723 WP_409289550.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38396.22 Da        Isoelectric Point: 7.3351

>NTDB_id=929515 V1501_RS15625 WP_409289547.1 3082165..3083217(-) (pilT) [Peribacillus sp. SCS-37]
MAIDIEHILRAGYELKASDIHLTVGVPPVMRINGDLKRYGKDALAPGDTEDMARKIVPGDMWESFKERGELDFSYGLAGV
SRFRVNAYHQRACISLAIRIVPRRIPALDELGLPEVLKRVAEKPHGLVLVTGPTGSGKSTTLASMISYINQTMKKHIVTL
EDPIEYLHKHGSCIIDQREVGFDTNSFANGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVFGTLHTSSAPSTID
RIIDVFPAHQQPQIRIQLASVLVAIVSQRLFPTAARDGRAAATEILINNPAIANLIRNEKIHQIASVIQTSKAQGMHTLE
SQIKDMLAAGRISREAASVFLQEKIENGQI

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=929515 V1501_RS15625 WP_409289547.1 3082165..3083217(-) (pilT) [Peribacillus sp. SCS-37]
ATGGCTATAGATATTGAACATATACTTCGTGCAGGCTATGAATTAAAGGCATCTGATATCCACCTGACCGTTGGGGTCCC
GCCTGTTATGCGCATAAACGGCGACCTGAAACGATATGGAAAGGACGCGCTGGCACCCGGGGATACAGAGGATATGGCAA
GGAAAATTGTACCCGGGGATATGTGGGAATCCTTCAAAGAGCGGGGGGAGCTCGACTTTTCTTACGGTCTCGCAGGGGTT
TCACGGTTCAGGGTGAATGCGTACCACCAGCGGGCTTGTATCTCGCTTGCCATCCGCATCGTACCTAGGAGGATTCCGGC
CCTCGATGAGCTTGGACTGCCGGAGGTGCTGAAAAGGGTGGCGGAGAAGCCGCACGGCCTCGTCCTTGTGACTGGTCCAA
CCGGAAGCGGCAAGTCGACGACGCTTGCCTCAATGATTTCGTACATAAACCAGACGATGAAAAAGCATATCGTGACTCTT
GAGGATCCGATTGAATATCTGCATAAGCACGGCAGCTGCATCATTGACCAGCGGGAAGTCGGCTTTGACACCAACAGTTT
TGCCAACGGACTCCGGGCAGCGCTCCGCCAGGATCCAGACGTGATCCTCGTCGGGGAAATGCGGGATCTTGAAACGATTC
AGACAGCCATCACCGCCGCGGAGACAGGCCATCTTGTTTTCGGGACTCTCCATACGTCCAGTGCGCCGTCGACTATCGAT
AGAATCATAGATGTCTTCCCGGCTCACCAGCAGCCGCAGATCCGCATCCAGCTGGCATCAGTGCTCGTGGCCATCGTCTC
CCAGCGGCTGTTTCCGACGGCCGCACGCGACGGACGGGCCGCGGCGACTGAGATATTGATCAATAATCCGGCAATCGCCA
ATTTGATACGCAATGAGAAAATCCACCAGATTGCCAGTGTGATTCAGACATCAAAGGCCCAGGGGATGCATACACTGGAA
AGCCAGATCAAGGACATGCTTGCTGCTGGACGGATATCCCGTGAAGCGGCTTCCGTCTTTTTACAGGAGAAGATAGAAAA
TGGCCAGATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.067

93.143

0.494
  pilT Vibrio cholerae strain A1552

49.419

98.286

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.419

98.286

0.486
  pilT Pseudomonas aeruginosa PAK

49.851

95.714

0.477
  pilT Pseudomonas stutzeri DSM 10701

49.254

95.714

0.471
  pilT Acinetobacter baumannii strain A118

48.358

95.714

0.463
  pilT Neisseria gonorrhoeae MS11

48.358

95.714

0.463
  pilT Neisseria meningitidis 8013

48.358

95.714

0.463
  pilT Acinetobacter baumannii D1279779

48.358

95.714

0.463
  pilT Acinetobacter nosocomialis M2

48.06

95.714

0.46
  pilT Legionella pneumophila strain Lp02

47.761

95.714

0.457
  pilT Legionella pneumophila strain ERS1305867

47.761

95.714

0.457
  pilT Acinetobacter baylyi ADP1

48.338

94.571

0.457
  pilU Pseudomonas stutzeri DSM 10701

42.773

96.857

0.414
  pilU Acinetobacter baylyi ADP1

41.265

94.857

0.391
  pilU Vibrio cholerae strain A1552

40.683

92

0.374