Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1499_RS15695 Genome accession   NZ_CP143543
Coordinates   3096379..3097416 (-) Length   345 a.a.
NCBI ID   WP_409270812.1    Uniprot ID   -
Organism   Neobacillus sp. SCS-31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3091379..3102416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1499_RS15670 (V1499_15670) - 3092095..3092730 (-) 636 WP_409270807.1 PilN domain-containing protein -
  V1499_RS15675 (V1499_15675) pilM 3092730..3093725 (-) 996 WP_409270808.1 type IV pilus biogenesis protein PilM -
  V1499_RS15680 (V1499_15680) - 3093803..3094549 (-) 747 WP_409270809.1 prepilin peptidase -
  V1499_RS15685 (V1499_15685) - 3094554..3095048 (-) 495 WP_409270810.1 type II secretion system protein -
  V1499_RS15690 (V1499_15690) - 3095162..3096376 (-) 1215 WP_409270811.1 type II secretion system F family protein -
  V1499_RS15695 (V1499_15695) pilT 3096379..3097416 (-) 1038 WP_409270812.1 type IV pilus twitching motility protein PilT Machinery gene
  V1499_RS15700 (V1499_15700) - 3097427..3099088 (-) 1662 WP_409270813.1 GspE/PulE family protein -
  V1499_RS15705 (V1499_15705) - 3099112..3100470 (-) 1359 WP_409270814.1 VanW family protein -
  V1499_RS15710 (V1499_15710) - 3100506..3101312 (-) 807 WP_409270815.1 hypothetical protein -
  V1499_RS15715 (V1499_15715) - 3101459..3101893 (+) 435 WP_409270816.1 type II secretion system protein J -
  V1499_RS15720 (V1499_15720) - 3101897..3102352 (+) 456 WP_409270817.1 PilW family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38422.53 Da        Isoelectric Point: 8.1932

>NTDB_id=929473 V1499_RS15695 WP_409270812.1 3096379..3097416(-) (pilT) [Neobacillus sp. SCS-31]
MKEKIDLILRAAFEHKASDIHMTVGIPPVFRINGELKKYGKEPLMPADTEGMARSIVPVDKWNAFKEHGELDFSYSISGL
SRFRVNTYHQRNCIAMALRTVPSKIPTIEELGMPETLHKLAEKPQGLVLVTGPTGSGKSTTLASMIHYMNMAMRKHIITL
EDPIEYLHKHGNSIIDQREVGMDTGNFANGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTSSAPATIN
RILDVFPPAQQAQIRIQLASVLVGIISQRLFPTTDRRGRKAATEIMINNAAIANLIRNEKVHQIPSVMQTSRALGMHTLE
SNIKELIEKGFIHREVAEPYFQEMV

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=929473 V1499_RS15695 WP_409270812.1 3096379..3097416(-) (pilT) [Neobacillus sp. SCS-31]
ATGAAGGAGAAAATTGACCTGATTTTAAGGGCCGCGTTTGAACATAAAGCATCGGATATCCACATGACAGTGGGCATCCC
GCCTGTTTTTCGGATTAACGGGGAACTGAAAAAGTATGGAAAGGAACCATTGATGCCAGCAGACACGGAGGGAATGGCAA
GGTCGATTGTTCCGGTTGATAAGTGGAATGCCTTTAAAGAGCACGGAGAATTGGATTTTTCGTATAGTATTTCCGGTTTA
TCGCGGTTCCGTGTTAACACGTACCACCAGCGAAACTGCATTGCGATGGCTCTTAGGACGGTTCCATCGAAGATTCCGAC
GATTGAGGAGCTTGGCATGCCCGAAACATTGCACAAGCTTGCCGAAAAACCACAGGGGCTTGTGCTCGTTACAGGCCCAA
CCGGGAGCGGAAAATCGACGACACTCGCTTCAATGATCCATTACATGAACATGGCGATGCGTAAGCATATCATTACACTC
GAAGATCCGATTGAGTACCTTCATAAACACGGGAATTCGATCATTGACCAGCGTGAAGTCGGGATGGATACCGGCAATTT
CGCAAATGGCCTCCGCGCCGCTCTGCGACAGGATCCGGACGTCATCCTTGTCGGGGAGATGCGCGATCTTGAAACGATTC
AGACGGCGATTACCGCAGCGGAAACCGGCCACCTTGTACTTGGAACGCTTCATACATCAAGCGCACCGGCCACAATCAAC
CGAATCTTGGACGTGTTTCCGCCGGCGCAGCAGGCCCAGATTCGGATTCAGCTGGCATCCGTTCTGGTCGGGATTATTTC
ACAGCGGTTGTTTCCGACCACCGACAGACGAGGGAGAAAGGCCGCGACCGAAATTATGATTAACAACGCGGCAATCGCTA
ACCTAATCCGCAACGAAAAAGTTCATCAGATACCAAGTGTCATGCAAACGTCGCGCGCCCTCGGGATGCACACGCTTGAG
TCGAACATAAAAGAACTGATTGAAAAAGGCTTTATTCATAGAGAAGTGGCGGAGCCATATTTTCAGGAGATGGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.497

96.812

0.499
  pilT Pseudomonas aeruginosa PAK

48.955

97.101

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.917

97.391

0.467
  pilT Vibrio cholerae strain A1552

49.231

94.203

0.464
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.231

94.203

0.464
  pilT Neisseria meningitidis 8013

46.707

96.812

0.452
  pilT Legionella pneumophila strain Lp02

46.97

95.652

0.449
  pilT Legionella pneumophila strain ERS1305867

46.97

95.652

0.449
  pilT Neisseria gonorrhoeae MS11

46.108

96.812

0.446
  pilT Acinetobacter baylyi ADP1

47.239

94.493

0.446
  pilT Acinetobacter nosocomialis M2

46.061

95.652

0.441
  pilT Acinetobacter baumannii D1279779

46.061

95.652

0.441
  pilT Acinetobacter baumannii strain A118

46.061

95.652

0.441
  pilU Pseudomonas stutzeri DSM 10701

43.03

95.652

0.412
  pilU Vibrio cholerae strain A1552

40.123

93.913

0.377
  pilU Acinetobacter baylyi ADP1

40.19

91.594

0.368