Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1498_RS05720 Genome accession   NZ_CP143542
Coordinates   1148907..1149959 (+) Length   350 a.a.
NCBI ID   WP_409297427.1    Uniprot ID   -
Organism   Peribacillus sp. SCS-26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1143907..1154959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1498_RS05705 (V1498_05705) - 1145077..1145790 (+) 714 WP_409297421.1 PRC-barrel domain-containing protein -
  V1498_RS05710 (V1498_05710) - 1145811..1147205 (+) 1395 WP_409297423.1 hypothetical protein -
  V1498_RS05715 (V1498_05715) - 1147234..1148895 (+) 1662 WP_409297425.1 GspE/PulE family protein -
  V1498_RS05720 (V1498_05720) pilT 1148907..1149959 (+) 1053 WP_409297427.1 type IV pilus twitching motility protein PilT Machinery gene
  V1498_RS05725 (V1498_05725) - 1149946..1151157 (+) 1212 WP_409297429.1 type II secretion system F family protein -
  V1498_RS05730 (V1498_05730) - 1151271..1151741 (+) 471 WP_409297431.1 type II secretion system protein -
  V1498_RS05735 (V1498_05735) - 1151742..1152497 (+) 756 WP_409297433.1 prepilin peptidase -
  V1498_RS05740 (V1498_05740) pilM 1152522..1153514 (+) 993 WP_409297434.1 type IV pilus biogenesis protein PilM -
  V1498_RS05745 (V1498_05745) - 1153517..1154194 (+) 678 WP_409297436.1 PilN domain-containing protein -
  V1498_RS05750 (V1498_05750) - 1154191..1154919 (+) 729 WP_409297437.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38401.28 Da        Isoelectric Point: 7.3014

>NTDB_id=929399 V1498_RS05720 WP_409297427.1 1148907..1149959(+) (pilT) [Peribacillus sp. SCS-26]
MAIDIEHILRAGYELKASDIHLTVGVPPVMRINGDLKRYGKDVLAPGDTENMARKIVPGDMWETFKERGELDFSYGLAGV
SRFRVNAYHQRACISLAIRIVPRRIPALDELGLPEVLKKVAEKPHGLVLVTGPTGSGKSTTLASMISYINQTMKKHIVTL
EDPIEYLHKHGSCIIDQREIGFDTNSFANGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVFGTLHTSSAPSTID
RIIDVFPADQQPQIRIQLASVLVAIVSQRLFPTAARDGRAAATEILINNPAIANLIRNEKIHQIASVIQTSKAQGMHTLE
SQIKDMLAAGRISREAASVFLQEKIENGQI

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=929399 V1498_RS05720 WP_409297427.1 1148907..1149959(+) (pilT) [Peribacillus sp. SCS-26]
ATGGCAATTGATATTGAACATATACTTCGTGCAGGCTATGAATTGAAGGCATCTGATATCCACCTGACCGTTGGGGTCCC
GCCTGTTATGCGCATAAACGGCGATCTGAAGCGGTATGGAAAGGACGTTCTGGCACCCGGGGATACAGAGAATATGGCAA
GGAAAATTGTACCCGGGGATATGTGGGAAACTTTCAAGGAGCGGGGGGAGCTTGACTTTTCTTACGGTCTCGCAGGGGTT
TCGCGGTTCAGGGTGAATGCATACCACCAGCGGGCTTGCATATCGCTTGCCATCCGCATCGTGCCGAGGCGGATTCCGGC
CCTTGATGAGCTGGGACTGCCGGAGGTGCTGAAAAAGGTGGCAGAGAAGCCGCACGGCCTCGTCCTGGTGACTGGTCCAA
CCGGGAGCGGGAAGTCTACGACGCTTGCCTCGATGATTTCGTACATAAACCAGACGATGAAAAAGCATATCGTGACCCTG
GAGGATCCGATTGAATATCTGCATAAGCACGGCAGCTGCATTATTGACCAGCGGGAAATCGGCTTTGACACCAACAGCTT
TGCCAACGGACTCCGGGCAGCGCTCCGCCAGGATCCTGATGTGATTCTTGTTGGCGAAATGCGTGATCTTGAAACGATCC
AGACAGCCATCACCGCAGCGGAGACAGGCCACCTCGTTTTCGGGACTCTCCATACATCCAGTGCGCCGTCGACAATCGAT
AGGATCATAGATGTCTTCCCGGCCGACCAGCAGCCGCAGATCCGCATTCAGCTGGCATCGGTACTCGTGGCCATCGTCTC
CCAGCGGCTGTTTCCGACGGCCGCCCGCGACGGACGTGCCGCCGCGACCGAGATATTGATCAATAATCCGGCGATCGCCA
ATTTGATACGCAATGAGAAAATCCACCAGATTGCCAGTGTGATCCAGACATCAAAGGCACAGGGGATGCATACACTGGAA
AGCCAGATCAAGGACATGCTTGCTGCCGGACGGATATCCCGTGAAGCGGCTTCCGTCTTTTTACAGGAGAAGATAGAAAA
TGGCCAGATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.761

93.143

0.491
  pilT Vibrio cholerae strain A1552

49.128

98.286

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.128

98.286

0.483
  pilT Pseudomonas aeruginosa PAK

49.254

95.714

0.471
  pilT Pseudomonas stutzeri DSM 10701

48.955

95.714

0.469
  pilT Neisseria gonorrhoeae MS11

48.955

95.714

0.469
  pilT Neisseria meningitidis 8013

48.955

95.714

0.469
  pilT Acinetobacter baumannii strain A118

47.761

95.714

0.457
  pilT Acinetobacter baumannii D1279779

47.761

95.714

0.457
  pilT Legionella pneumophila strain ERS1305867

47.463

95.714

0.454
  pilT Legionella pneumophila strain Lp02

47.463

95.714

0.454
  pilT Acinetobacter nosocomialis M2

47.463

95.714

0.454
  pilT Acinetobacter baylyi ADP1

48.036

94.571

0.454
  pilU Pseudomonas stutzeri DSM 10701

43.068

96.857

0.417
  pilU Acinetobacter baylyi ADP1

41.265

94.857

0.391
  pilU Vibrio cholerae strain A1552

40.062

92

0.369