Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1680_RS10665 Genome accession   NZ_CP143534
Coordinates   2440530..2441582 (-) Length   350 a.a.
NCBI ID   WP_329743779.1    Uniprot ID   -
Organism   Clostridium perfringens strain CQ92     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2435530..2446582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1680_RS10645 (V1680_10645) sigE 2436123..2436830 (-) 708 WP_003449468.1 RNA polymerase sporulation sigma factor SigE -
  V1680_RS10650 (V1680_10650) spoIIGA 2436844..2437650 (-) 807 WP_061428817.1 sigma-E processing peptidase SpoIIGA -
  V1680_RS10655 (V1680_10655) ftsZ 2438055..2439200 (-) 1146 WP_061415801.1 cell division protein FtsZ -
  V1680_RS10660 (V1680_10660) - 2439214..2440452 (-) 1239 WP_330438606.1 cell division FtsA domain-containing protein -
  V1680_RS10665 (V1680_10665) pilT 2440530..2441582 (-) 1053 WP_329743779.1 type IV pilus twitching motility protein PilT Machinery gene
  V1680_RS10670 (V1680_10670) sigK 2441763..2442464 (-) 702 WP_330438607.1 RNA polymerase sporulation sigma factor SigK -
  V1680_RS10675 (V1680_10675) - 2442527..2444194 (-) 1668 WP_124041234.1 penicillin-binding transpeptidase domain-containing protein -
  V1680_RS10680 (V1680_10680) udk 2444312..2444938 (-) 627 WP_060794470.1 uridine kinase -
  V1680_RS10685 (V1680_10685) - 2444938..2446167 (-) 1230 WP_003459619.1 peptidase U32 family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39004.68 Da        Isoelectric Point: 5.4480

>NTDB_id=929338 V1680_RS10665 WP_329743779.1 2440530..2441582(-) (pilT) [Clostridium perfringens strain CQ92]
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNIKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQTIISQQLVETVDGDRTAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=929338 V1680_RS10665 WP_329743779.1 2440530..2441582(-) (pilT) [Clostridium perfringens strain CQ92]
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTACGCCCTGAAGATACTGAAGCATATGCTAGAG
AGATATTACAGGATGCATATGAAAAGTATGATGCTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGAGTTATATCTTTAGATATGCCAACTCTAGA
TAGTTTAGGATATCCTGAGACTTTAAAGGATATATGTAATATTAAAAGGGGCCTTGTATTAGTTACAGGACCTACTGGAA
GTGGTAAAAGTACAACTTTAGCTGCTTTAATAAATGAAATAAATAGTAATAGGGAATCTCATATAATAACAATAGAAGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAGAGAGAGATAGGGAAGGACACTCTAAGTTATGAAAG
AGCCTTAAAAGCAGCACTTAGAGAGGATCCTGATGTTATTTTAATTGGAGAAATGAGAGATTTAGAGACTATTTCAACAG
CTATAACAGCAGCAGAGACTGGACACTTAGTGTTTTCAACTCTTCATACTATAGGAGCAGCTAAGACTATAGATAGAATA
GTAGATGTTTTTCCACCACATCAACAAGAGCAAATAAAAATACAGTTAGCTTCCGTTTTACAAACAATTATATCACAGCA
GTTAGTAGAAACAGTTGATGGAGATAGAACTGCGGCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAATTTAATAA
GAGAAGGAAAAACTCATCAAATAGAATCATCCATACAAACAGGAAGTAAATATGGAATGAGAACAATGGACATGGAACTT
GCTAATCTTTATAGAGAAGGTATAATAACACAGGAAACTGCAATGAACTCTGCTATAGATAGGGAGATTCTAAGTCGATT
ATTGATGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.711

98.857

0.491
  pilT Neisseria meningitidis 8013

49.709

98.286

0.489
  pilT Neisseria gonorrhoeae MS11

49.709

98.286

0.489
  pilT Pseudomonas aeruginosa PAK

50.298

96

0.483
  pilT Legionella pneumophila strain Lp02

49.415

97.714

0.483
  pilT Legionella pneumophila strain ERS1305867

49.415

97.714

0.483
  pilT Pseudomonas stutzeri DSM 10701

50

96

0.48
  pilT Acinetobacter baylyi ADP1

50.602

94.857

0.48
  pilT Acinetobacter baumannii D1279779

49.702

96

0.477
  pilT Acinetobacter nosocomialis M2

49.702

96

0.477
  pilT Acinetobacter baumannii strain A118

49.702

96

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

93.429

0.474
  pilT Vibrio cholerae strain A1552

50.765

93.429

0.474
  pilU Pseudomonas stutzeri DSM 10701

43.976

94.857

0.417
  pilU Vibrio cholerae strain A1552

40.653

96.286

0.391
  pilU Acinetobacter baylyi ADP1

39.589

97.429

0.386