Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AMOR_RS16425 Genome accession   NZ_AP025591
Coordinates   3754220..3755317 (+) Length   365 a.a.
NCBI ID   WP_248352636.1    Uniprot ID   -
Organism   Anaeromyxobacter oryzae strain Red232     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3749220..3760317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMOR_RS16400 (AMOR_32640) - 3749557..3750405 (+) 849 WP_248352631.1 purine-nucleoside phosphorylase -
  AMOR_RS16405 (AMOR_32650) - 3750452..3750841 (+) 390 WP_248352632.1 PilZ domain-containing protein -
  AMOR_RS16410 (AMOR_32660) - 3750838..3751245 (+) 408 WP_248352633.1 cytidine deaminase -
  AMOR_RS16415 (AMOR_32670) ribF 3751320..3752282 (+) 963 WP_248352634.1 riboflavin biosynthesis protein RibF -
  AMOR_RS16420 (AMOR_32680) pilB 3752486..3754192 (+) 1707 WP_248352635.1 type IV-A pilus assembly ATPase PilB -
  AMOR_RS16425 (AMOR_32690) pilT 3754220..3755317 (+) 1098 WP_248352636.1 type IV pilus twitching motility protein PilT Machinery gene
  AMOR_RS16430 (AMOR_32700) - 3755394..3756647 (+) 1254 WP_248352637.1 type II secretion system F family protein -
  AMOR_RS16435 (AMOR_32710) - 3756653..3758299 (+) 1647 WP_248352638.1 nitrogen regulation protein NR(II) -
  AMOR_RS16440 (AMOR_32720) - 3758309..3759679 (+) 1371 WP_248352639.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40015.40 Da        Isoelectric Point: 9.2811

>NTDB_id=92903 AMOR_RS16425 WP_248352636.1 3754220..3755317(+) (pilT) [Anaeromyxobacter oryzae strain Red232]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRIDGKLVPLKTPPMSPVDTKQLCYSILTDAQKHKFEEENELDLSFGVKGLS
RFRANVFMQRGAVAGAFRTIPFKILTFAELGLPPIVADLAKKPRGLVLVTGPTGSGKSTTLASMIDKINTERHEHIITIE
DPIEYLHPHKNCVVNQREVGADTASFKKALKYILRQDPDVVLVGEMRDLETVEAALVISETGHLAFATLHTNSAVQTINR
ILDVFPPYQQPQVRAQLSFVLEGVLTQNLLPKAGGPGRVLIIEVMVPNPAIRNLIREDKVHQVYSQMQVGQTKFGMQTFN
QSLAAAFAKRLITLEEAMGRSSDPDELKNLLSNGGGMRPPPAAAR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=92903 AMOR_RS16425 WP_248352636.1 3754220..3755317(+) (pilT) [Anaeromyxobacter oryzae strain Red232]
GTGGCGAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGGGCCTCCGACCTCCACGTCACGACCGGCTCGCCGCC
GCAGCTCCGGATCGACGGAAAGCTCGTCCCGCTCAAGACGCCGCCGATGTCCCCGGTGGACACGAAGCAGCTCTGCTACT
CGATCCTCACCGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAGCTCGACCTGTCGTTCGGCGTGAAGGGGCTGTCG
CGGTTCCGCGCGAACGTCTTCATGCAGCGCGGCGCGGTCGCCGGCGCGTTCCGCACCATCCCGTTCAAGATCCTCACCTT
CGCCGAGCTGGGGCTCCCCCCCATCGTGGCCGATCTCGCGAAGAAGCCCCGCGGGCTGGTCCTCGTCACGGGCCCGACCG
GCTCCGGGAAGTCCACCACGCTCGCGTCGATGATCGACAAGATCAACACCGAGCGCCACGAGCACATCATCACGATCGAG
GATCCGATCGAGTACCTCCACCCCCACAAGAACTGCGTCGTGAACCAGCGCGAGGTGGGGGCGGACACGGCGTCGTTCAA
GAAGGCGCTCAAGTACATCCTCCGGCAGGATCCCGACGTCGTGCTCGTCGGCGAGATGCGCGACCTCGAGACCGTCGAGG
CGGCCCTCGTCATCTCCGAGACCGGTCACCTCGCGTTCGCGACGCTGCACACGAACTCCGCCGTCCAGACCATCAACCGC
ATCCTCGACGTGTTCCCCCCGTACCAGCAGCCGCAGGTCCGCGCGCAGCTCTCGTTCGTGCTCGAGGGCGTGCTCACCCA
GAACCTGCTCCCGAAGGCGGGCGGGCCGGGGCGCGTCCTCATCATCGAGGTGATGGTGCCGAACCCGGCGATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGGTCTACTCGCAGATGCAGGTCGGCCAGACGAAGTTCGGGATGCAGACGTTCAAC
CAGAGCCTCGCGGCGGCGTTCGCGAAGCGGCTCATCACGCTCGAGGAGGCGATGGGGCGCTCCTCCGATCCGGACGAGCT
CAAGAACCTGCTCTCGAACGGTGGCGGGATGCGCCCGCCGCCCGCAGCGGCCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.055

100

0.521
  pilT Acinetobacter baylyi ADP1

51.594

94.521

0.488
  pilT Legionella pneumophila strain Lp02

52.87

90.685

0.479
  pilT Legionella pneumophila strain ERS1305867

52.87

90.685

0.479
  pilT Pseudomonas aeruginosa PAK

50.435

94.521

0.477
  pilT Acinetobacter baumannii strain A118

50.145

94.521

0.474
  pilT Pseudomonas stutzeri DSM 10701

50.145

94.521

0.474
  pilT Acinetobacter baumannii D1279779

50.145

94.521

0.474
  pilT Acinetobacter nosocomialis M2

49.855

94.521

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.817

92.603

0.452
  pilT Vibrio cholerae strain A1552

48.817

92.603

0.452
  pilT Neisseria gonorrhoeae MS11

46.334

93.425

0.433
  pilT Neisseria meningitidis 8013

46.334

93.425

0.433
  pilU Pseudomonas stutzeri DSM 10701

42.735

96.164

0.411
  pilU Vibrio cholerae strain A1552

41.643

96.712

0.403
  pilU Acinetobacter baylyi ADP1

39.193

95.068

0.373

Multiple sequence alignment