Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1234_RS21480 Genome accession   NZ_CP143341
Coordinates   4485335..4486357 (-) Length   340 a.a.
NCBI ID   WP_330113070.1    Uniprot ID   -
Organism   Serratia marcescens strain 2023CB-00608     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4480335..4491357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1234_RS21450 (V1234_21450) - 4481136..4481648 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  V1234_RS21455 (V1234_21455) endA 4481750..4482445 (+) 696 WP_038879275.1 deoxyribonuclease I -
  V1234_RS21460 (V1234_21460) rsmE 4482515..4483246 (+) 732 WP_070913720.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  V1234_RS21465 (V1234_21465) gshB 4483257..4484207 (+) 951 WP_047729854.1 glutathione synthase -
  V1234_RS21470 (V1234_21470) - 4484353..4484916 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  V1234_RS21475 (V1234_21475) ruvX 4484916..4485338 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  V1234_RS21480 (V1234_21480) pilT 4485335..4486357 (-) 1023 WP_330113070.1 type IV pilus twitching motility protein PilT Machinery gene
  V1234_RS21485 (V1234_21485) - 4486378..4487085 (+) 708 WP_102985233.1 YggS family pyridoxal phosphate-dependent enzyme -
  V1234_RS21490 (V1234_21490) proC 4487105..4487926 (+) 822 WP_060422650.1 pyrroline-5-carboxylate reductase -
  V1234_RS21495 (V1234_21495) - 4487958..4488512 (+) 555 WP_084827144.1 YggT family protein -
  V1234_RS21500 (V1234_21500) yggU 4488509..4488799 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  V1234_RS21505 (V1234_21505) - 4488817..4489410 (+) 594 WP_180547705.1 XTP/dITP diphosphatase -
  V1234_RS21510 (V1234_21510) hemW 4489403..4490545 (+) 1143 WP_084827142.1 radical SAM family heme chaperone HemW -
  V1234_RS21515 (V1234_21515) - 4490583..4491017 (-) 435 WP_016930094.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36457.82 Da        Isoelectric Point: 7.2889

>NTDB_id=928689 V1234_RS21480 WP_330113070.1 4485335..4486357(-) (pilT) [Serratia marcescens strain 2023CB-00608]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQIDLALHRPGGE
RLRANVFQQRAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDTHSFDAALRAALREDPDVILLGELRDVATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=928689 V1234_RS21480 WP_330113070.1 4485335..4486357(-) (pilT) [Serratia marcescens strain 2023CB-00608]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGATCGATCTGGCGCTGCACAGGCCGGGTGGGGAG
CGGCTGCGAGCCAACGTTTTTCAGCAACGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCCCCGGCCATCGTCCCGGCACTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTAACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATTCAACAGCGGGAGATCGGCCGCGACACTCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGTTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATGTCGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGCGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Acinetobacter baylyi ADP1

51.662

97.353

0.503
  pilT Neisseria meningitidis 8013

50.448

98.529

0.497
  pilT Acinetobacter nosocomialis M2

51.212

97.059

0.497
  pilT Acinetobacter baumannii D1279779

51.212

97.059

0.497
  pilT Acinetobacter baumannii strain A118

51.212

97.059

0.497
  pilT Neisseria gonorrhoeae MS11

50.453

97.353

0.491
  pilT Pseudomonas aeruginosa PAK

50

97.059

0.485
  pilT Legionella pneumophila strain ERS1305867

49.547

97.353

0.482
  pilT Legionella pneumophila strain Lp02

49.547

97.353

0.482
  pilT Pseudomonas stutzeri DSM 10701

49.394

97.059

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.614

100

0.459
  pilU Vibrio cholerae strain A1552

40.491

95.882

0.388
  pilU Acinetobacter baylyi ADP1

40.557

95

0.385
  pilU Pseudomonas stutzeri DSM 10701

39.506

95.294

0.376