Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V0I27_RS10450 Genome accession   NZ_CP143277
Coordinates   2401047..2402099 (-) Length   350 a.a.
NCBI ID   WP_329743779.1    Uniprot ID   -
Organism   Clostridium perfringens strain SXD-8213     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2402280..2440138 2401047..2402099 flank 181


Gene organization within MGE regions


Location: 2401047..2440138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V0I27_RS10450 (V0I27_10450) pilT 2401047..2402099 (-) 1053 WP_329743779.1 type IV pilus twitching motility protein PilT Machinery gene
  V0I27_RS10455 (V0I27_10455) - 2402280..2402552 (-) 273 Protein_2044 sigma-70 family RNA polymerase sigma factor -
  V0I27_RS10460 (V0I27_10460) - 2402641..2402877 (-) 237 WP_329743780.1 hypothetical protein -
  V0I27_RS10465 (V0I27_10465) - 2402954..2403304 (-) 351 WP_329743781.1 hypothetical protein -
  V0I27_RS10470 (V0I27_10470) - 2403319..2404107 (-) 789 WP_329743782.1 hypothetical protein -
  V0I27_RS10475 (V0I27_10475) - 2404254..2404433 (-) 180 WP_329743783.1 hypothetical protein -
  V0I27_RS10480 (V0I27_10480) - 2404861..2405889 (-) 1029 WP_329743784.1 GH25 family lysozyme -
  V0I27_RS10485 (V0I27_10485) - 2406158..2406886 (-) 729 WP_329743785.1 SH3 domain-containing protein -
  V0I27_RS10490 (V0I27_10490) - 2407028..2407405 (-) 378 WP_329743786.1 hypothetical protein -
  V0I27_RS10495 (V0I27_10495) - 2407488..2408015 (-) 528 WP_329743787.1 sigma-70 family RNA polymerase sigma factor -
  V0I27_RS10500 (V0I27_10500) - 2408244..2408735 (-) 492 WP_329743788.1 cobalt ABC transporter permease -
  V0I27_RS10505 (V0I27_10505) - 2408756..2408989 (-) 234 WP_011590771.1 hemolysin XhlA family protein -
  V0I27_RS10510 (V0I27_10510) - 2409003..2409269 (-) 267 WP_078209910.1 hypothetical protein -
  V0I27_RS10515 (V0I27_10515) - 2409284..2417905 (-) 8622 WP_329743789.1 phage tail spike protein -
  V0I27_RS10520 (V0I27_10520) - 2417923..2418156 (-) 234 WP_329743790.1 hypothetical protein -
  V0I27_RS10525 (V0I27_10525) - 2418221..2418514 (-) 294 WP_329743791.1 hypothetical protein -
  V0I27_RS10530 (V0I27_10530) - 2418540..2419241 (-) 702 WP_329743792.1 phage distal tail protein -
  V0I27_RS10535 (V0I27_10535) - 2419243..2422782 (-) 3540 WP_329743793.1 phage tail tape measure protein -
  V0I27_RS10540 (V0I27_10540) - 2422801..2422950 (-) 150 WP_167875052.1 hypothetical protein -
  V0I27_RS10545 (V0I27_10545) - 2423007..2423381 (-) 375 WP_329743794.1 hypothetical protein -
  V0I27_RS10550 (V0I27_10550) - 2423395..2424000 (-) 606 WP_224396946.1 major tail protein -
  V0I27_RS10555 (V0I27_10555) - 2424372..2424731 (-) 360 WP_323228102.1 hypothetical protein -
  V0I27_RS10560 (V0I27_10560) - 2424792..2425139 (-) 348 WP_329743795.1 DUF4064 domain-containing protein -
  V0I27_RS10565 (V0I27_10565) - 2425186..2425527 (-) 342 WP_329743796.1 phage head closure protein -
  V0I27_RS10570 (V0I27_10570) - 2425527..2425826 (-) 300 WP_329743797.1 head-tail connector protein -
  V0I27_RS10575 (V0I27_10575) - 2425829..2426998 (-) 1170 WP_329743798.1 phage major capsid protein -
  V0I27_RS10580 (V0I27_10580) - 2427033..2427854 (-) 822 WP_329743799.1 head maturation protease, ClpP-related -
  V0I27_RS10585 (V0I27_10585) - 2427841..2429106 (-) 1266 WP_329743800.1 phage portal protein -
  V0I27_RS10590 (V0I27_10590) - 2429125..2430855 (-) 1731 WP_329743801.1 terminase large subunit -
  V0I27_RS10595 (V0I27_10595) - 2430864..2431385 (-) 522 WP_329743802.1 phage terminase small subunit P27 family -
  V0I27_RS10600 (V0I27_10600) - 2431489..2431923 (-) 435 WP_329743804.1 HNH endonuclease -
  V0I27_RS10605 (V0I27_10605) - 2431916..2432110 (-) 195 WP_329743805.1 hypothetical protein -
  V0I27_RS10610 (V0I27_10610) - 2432743..2432907 (+) 165 WP_329743806.1 hypothetical protein -
  V0I27_RS10615 (V0I27_10615) - 2433287..2433727 (-) 441 WP_329743807.1 hypothetical protein -
  V0I27_RS10620 (V0I27_10620) - 2433759..2433917 (-) 159 WP_329743808.1 hypothetical protein -
  V0I27_RS10625 (V0I27_10625) - 2433929..2434204 (-) 276 WP_329743809.1 MarR family transcriptional regulator -
  V0I27_RS10630 (V0I27_10630) - 2434280..2434501 (-) 222 WP_329743810.1 hypothetical protein -
  V0I27_RS10635 (V0I27_10635) - 2434505..2435245 (-) 741 WP_329743811.1 hypothetical protein -
  V0I27_RS10640 (V0I27_10640) - 2435471..2435866 (-) 396 WP_329743812.1 hypothetical protein -
  V0I27_RS10645 (V0I27_10645) - 2435853..2436026 (-) 174 WP_329743813.1 hypothetical protein -
  V0I27_RS10650 (V0I27_10650) - 2436063..2436254 (-) 192 WP_329743814.1 hypothetical protein -
  V0I27_RS10655 (V0I27_10655) - 2436359..2436505 (-) 147 WP_329743815.1 hypothetical protein -
  V0I27_RS10660 (V0I27_10660) - 2436619..2436846 (-) 228 WP_329743816.1 helix-turn-helix transcriptional regulator -
  V0I27_RS10665 (V0I27_10665) - 2437094..2437456 (+) 363 WP_329743817.1 helix-turn-helix domain-containing protein -
  V0I27_RS10670 (V0I27_10670) - 2437831..2438049 (+) 219 WP_329743818.1 helix-turn-helix transcriptional regulator -
  V0I27_RS10675 (V0I27_10675) - 2438137..2439645 (+) 1509 WP_329743819.1 recombinase family protein -
  V0I27_RS10680 (V0I27_10680) - 2439692..2440138 (-) 447 Protein_2089 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39004.68 Da        Isoelectric Point: 5.4480

>NTDB_id=928483 V0I27_RS10450 WP_329743779.1 2401047..2402099(-) (pilT) [Clostridium perfringens strain SXD-8213]
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNIKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQTIISQQLVETVDGDRTAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=928483 V0I27_RS10450 WP_329743779.1 2401047..2402099(-) (pilT) [Clostridium perfringens strain SXD-8213]
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTACGCCCTGAAGATACTGAAGCATATGCTAGAG
AGATATTACAGGATGCATATGAAAAGTATGATGCTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGAGTTATATCTTTAGATATGCCAACTCTAGA
TAGTTTAGGATATCCTGAGACTTTAAAGGATATATGTAATATTAAAAGGGGCCTTGTATTAGTTACAGGACCTACTGGAA
GTGGTAAAAGTACAACTTTAGCTGCTTTAATAAATGAAATAAATAGTAATAGGGAATCTCATATAATAACAATAGAAGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAGAGAGAGATAGGGAAGGACACTCTAAGTTATGAAAG
AGCCTTAAAAGCAGCACTTAGAGAGGATCCTGATGTTATTTTAATTGGAGAAATGAGAGATTTAGAGACTATTTCAACAG
CTATAACAGCAGCAGAGACTGGACACTTAGTGTTTTCAACTCTTCATACTATAGGAGCAGCTAAGACTATAGATAGAATA
GTAGATGTTTTTCCACCACATCAACAAGAGCAAATAAAAATACAGTTAGCTTCCGTTTTACAAACAATTATATCACAGCA
GTTAGTAGAAACAGTTGATGGAGATAGAACTGCGGCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAATTTAATAA
GAGAAGGAAAAACTCATCAAATAGAATCATCCATACAAACAGGAAGTAAATATGGAATGAGAACAATGGACATGGAACTT
GCTAATCTTTATAGAGAAGGTATAATAACACAGGAAACTGCAATGAACTCTGCTATAGATAGGGAGATTCTAAGTCGATT
ATTGATGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.711

98.857

0.491
  pilT Neisseria meningitidis 8013

49.709

98.286

0.489
  pilT Neisseria gonorrhoeae MS11

49.709

98.286

0.489
  pilT Pseudomonas aeruginosa PAK

50.298

96

0.483
  pilT Legionella pneumophila strain Lp02

49.415

97.714

0.483
  pilT Legionella pneumophila strain ERS1305867

49.415

97.714

0.483
  pilT Pseudomonas stutzeri DSM 10701

50

96

0.48
  pilT Acinetobacter baylyi ADP1

50.602

94.857

0.48
  pilT Acinetobacter baumannii D1279779

49.702

96

0.477
  pilT Acinetobacter nosocomialis M2

49.702

96

0.477
  pilT Acinetobacter baumannii strain A118

49.702

96

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

93.429

0.474
  pilT Vibrio cholerae strain A1552

50.765

93.429

0.474
  pilU Pseudomonas stutzeri DSM 10701

43.976

94.857

0.417
  pilU Vibrio cholerae strain A1552

40.653

96.286

0.391
  pilU Acinetobacter baylyi ADP1

39.589

97.429

0.386