Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LMS42_RS01560 Genome accession   NZ_CP143271
Coordinates   331933..332985 (+) Length   350 a.a.
NCBI ID   WP_224377808.1    Uniprot ID   -
Organism   Clostridium perfringens strain P201     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326933..337985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMS42_RS01540 (LMS42_001540) - 327350..328579 (+) 1230 WP_115613601.1 peptidase U32 family protein -
  LMS42_RS01545 (LMS42_001545) udk 328579..329205 (+) 627 WP_003459577.1 uridine kinase -
  LMS42_RS01550 (LMS42_001550) - 329318..330988 (+) 1671 WP_228571881.1 penicillin-binding transpeptidase domain-containing protein -
  LMS42_RS01555 (LMS42_001555) sigK 331051..331752 (+) 702 WP_003451260.1 RNA polymerase sporulation sigma factor SigK -
  LMS42_RS01560 (LMS42_001560) pilT 331933..332985 (+) 1053 WP_224377808.1 type IV pilus twitching motility protein PilT Machinery gene
  LMS42_RS01565 (LMS42_001565) - 333063..334301 (+) 1239 WP_228571879.1 cell division FtsA domain-containing protein -
  LMS42_RS01570 (LMS42_001570) ftsZ 334315..335460 (+) 1146 WP_003449411.1 cell division protein FtsZ -
  LMS42_RS01575 (LMS42_001575) spoIIGA 335865..336671 (+) 807 WP_003458523.1 sigma-E processing peptidase SpoIIGA -
  LMS42_RS01580 (LMS42_001580) sigE 336685..337392 (+) 708 WP_003449468.1 RNA polymerase sporulation sigma factor SigE -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39057.78 Da        Isoelectric Point: 5.4480

>NTDB_id=928457 LMS42_RS01560 WP_224377808.1 331933..332985(+) (pilT) [Clostridium perfringens strain P201]
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEVYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQIIISQQLVETVDGDRNAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=928457 LMS42_RS01560 WP_224377808.1 331933..332985(+) (pilT) [Clostridium perfringens strain P201]
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTACGCCCTGAAGATACTGAAGTGTATGCTAGAG
AGATATTACAGGATGCATATGAAAAGTATGATGCTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGAGTTATATCTTTAGATATGCCAACTTTAGA
TAGTTTAGGATATCCTGAGACTTTAAAGGATATATGTAATCTAAAAAGAGGACTTGTATTAGTTACAGGACCTACTGGAA
GTGGTAAAAGTACAACTTTAGCAGCTTTAATAAATGAAATAAATAGTAATAGAGAATCTCATATAATAACAATAGAAGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAAAGAGAGATAGGCAAGGACACTCTAAGTTATGAAAG
AGCCTTAAAAGCAGCTCTTAGAGAGGATCCTGATGTTATTTTAATTGGAGAAATGAGAGATTTAGAGACTATTTCAACAG
CTATAACAGCGGCAGAGACTGGACACTTAGTATTTTCAACTCTTCATACTATAGGAGCTGCTAAGACTATAGATAGAATA
GTAGATGTTTTTCCACCACATCAGCAGGAGCAAATAAAAATACAGTTAGCTTCTGTTTTACAAATAATTATATCACAACA
ATTAGTGGAAACCGTTGATGGAGATAGAAATGCTGCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAATTTAATAA
GAGAAGGAAAAACTCATCAAATAGAATCTTCTATCCAAACTGGAAGTAAATATGGAATGAGAACGATGGACATGGAACTT
GCTAATCTGTATAGAGAAGGTATAATAACACAAGAAACGGCAATGAATTCTGCTATAGATAGGGAGATTTTAAGTCGATT
ATTGATGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.711

98.857

0.491
  pilT Neisseria meningitidis 8013

49.709

98.286

0.489
  pilT Neisseria gonorrhoeae MS11

49.709

98.286

0.489
  pilT Legionella pneumophila strain ERS1305867

49.415

97.714

0.483
  pilT Legionella pneumophila strain Lp02

49.415

97.714

0.483
  pilT Pseudomonas aeruginosa PAK

50

96

0.48
  pilT Acinetobacter baylyi ADP1

50.602

94.857

0.48
  pilT Acinetobacter baumannii strain A118

49.702

96

0.477
  pilT Pseudomonas stutzeri DSM 10701

49.702

96

0.477
  pilT Acinetobacter baumannii D1279779

49.702

96

0.477
  pilT Acinetobacter nosocomialis M2

49.702

96

0.477
  pilT Vibrio cholerae strain A1552

50.765

93.429

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

93.429

0.474
  pilU Pseudomonas stutzeri DSM 10701

43.976

94.857

0.417
  pilU Vibrio cholerae strain A1552

40.356

96.286

0.389
  pilU Acinetobacter baylyi ADP1

39.823

96.857

0.386