Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VXM60_RS14535 Genome accession   NZ_CP143082
Coordinates   3328770..3329807 (-) Length   345 a.a.
NCBI ID   WP_126167633.1    Uniprot ID   -
Organism   Shewanella khirikhana strain JW44     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3323770..3334807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VXM60_RS14500 (VXM60_14500) - 3324262..3324822 (-) 561 WP_126167627.1 DUF4136 domain-containing protein -
  VXM60_RS14505 (VXM60_14505) - 3325007..3325324 (+) 318 WP_126167628.1 hypothetical protein -
  VXM60_RS14510 (VXM60_14510) yciH 3325439..3325768 (+) 330 WP_126167629.1 stress response translation initiation inhibitor YciH -
  VXM60_RS14515 (VXM60_14515) - 3325773..3326336 (+) 564 WP_240591730.1 YqgE/AlgH family protein -
  VXM60_RS14520 (VXM60_14520) ruvX 3326336..3326755 (+) 420 WP_338589476.1 Holliday junction resolvase RuvX -
  VXM60_RS14525 (VXM60_14525) - 3326870..3327496 (-) 627 WP_338589478.1 glutathione peroxidase -
  VXM60_RS14530 (VXM60_14530) pilU 3327648..3328760 (-) 1113 WP_126167632.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VXM60_RS14535 (VXM60_14535) pilT 3328770..3329807 (-) 1038 WP_126167633.1 type IV pilus twitching motility protein PilT Machinery gene
  VXM60_RS14540 (VXM60_14540) - 3329843..3330592 (+) 750 WP_338589480.1 YggS family pyridoxal phosphate-dependent enzyme -
  VXM60_RS14545 (VXM60_14545) proC 3330669..3331484 (+) 816 WP_126167635.1 pyrroline-5-carboxylate reductase -
  VXM60_RS14550 (VXM60_14550) - 3331544..3332092 (+) 549 WP_338589482.1 YggT family protein -
  VXM60_RS14555 (VXM60_14555) yggU 3332092..3332379 (+) 288 WP_240591735.1 DUF167 family protein YggU -
  VXM60_RS14560 (VXM60_14560) - 3332460..3332894 (+) 435 WP_126167638.1 DUF4426 domain-containing protein -
  VXM60_RS14565 (VXM60_14565) rdgB 3333037..3333633 (+) 597 WP_126167639.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  VXM60_RS14570 (VXM60_14570) hemW 3333639..3334805 (+) 1167 WP_338589484.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38220.95 Da        Isoelectric Point: 7.0499

>NTDB_id=928153 VXM60_RS14535 WP_126167633.1 3328770..3329807(-) (pilT) [Shewanella khirikhana strain JW44]
MEITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHAGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSQILTLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAGMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
ANLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=928153 VXM60_RS14535 WP_126167633.1 3328770..3329807(-) (pilT) [Shewanella khirikhana strain JW44]
ATGGAAATCACAGAGTTACTGGCGTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTGTCTGCCGGGGTTCCCCCCAT
GATCCGTGTGGACGGCGAAGTGCGTAAAATCAATCTGCCTGCACTCGATCATGCCGGCGTACACTCCTTGGTGTACGACA
TTATGAATGACAAGCAGCGCAAAGACTATGAAGAGCACCTGGAAATCGACTTTTCCTTCGAAGTGCCCAATCTCGCCCGT
TTCCGTGTGAACGCCTTTAACCAGTCCCGCGGTGCGGCGGCGGTGTTCCGTACCATTCCCAGCCAGATCCTGACCCTTGA
GCAGCTGGGCGCGCCGGAAATCTTCAAAAAAATCGCCAGTTTCCCCCGTGGTCTGGTGCTGGTAACCGGCCCAACCGGTT
CCGGTAAAAGTACCACCCTGGCCGGTATGATTGACTACGTGAACGAAAACCGTCACGACCACATCCTGACCATCGAAGAC
CCTATCGAATTTGTGCATCAGAACAAACAGTGTCTGATTAACCAGCGTGAAGTGCATCGCCACACCCACAGCTTTAACGC
CGCCCTTCGAAGCGCGCTGCGTGAAGACCCCGACGTGATCCTTGTGGGTGAGATGCGTGACCTTGAAACTATTCGTCTGG
CCATGACAGCCGCAGAAACCGGTCACCTGGTTTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGTGTG
GTTGACGTATTCCCCGCCGGTGAAAAAGACATGGTGCGTACCATGTTGTCAGAGTCCCTGCAGGCCGTAATTTCCCAGAC
CCTGATCAAGAAGATTGGCGGCGGCCGGGTGGCGGCACACGAAATCATGATGGGTACTCCGGCTATCCGTAACCTTATCC
GTGAGGACAAGGTGGCGCAGATGTATTCGGCCATTCAAACCGGTATGGCCCATGGCATGCAGACCCTCGAGCAGTGTCTG
GCCAATCTGGTGAACCGTGGTCTCATCACCCGTGAAGATGCCATGGCCAAGAGTTCCAACAAGCAGGCGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.702

99.13

0.8
  pilT Acinetobacter baumannii D1279779

80.702

99.13

0.8
  pilT Acinetobacter baumannii strain A118

80.702

99.13

0.8
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baylyi ADP1

78.134

99.42

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.294

97.681

0.716
  pilT Vibrio cholerae strain A1552

73.294

97.681

0.716
  pilT Neisseria meningitidis 8013

68.605

99.71

0.684
  pilT Neisseria gonorrhoeae MS11

68.314

99.71

0.681
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.737

98.261

0.499
  pilU Pseudomonas stutzeri DSM 10701

42.857

99.42

0.426
  pilU Vibrio cholerae strain A1552

41.983

99.42

0.417
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391