Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VXM67_RS03680 Genome accession   NZ_CP142890
Coordinates   804690..805688 (+) Length   332 a.a.
NCBI ID   WP_027907908.1    Uniprot ID   -
Organism   Pseudomonas sp. Rh2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 799690..810688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VXM67_RS03650 (VXM67_03650) - 800167..800601 (-) 435 WP_023378207.1 DUF4426 domain-containing protein -
  VXM67_RS03655 (VXM67_03655) metW 800623..801243 (-) 621 WP_023378208.1 methionine biosynthesis protein MetW -
  VXM67_RS03660 (VXM67_03660) - 801251..802390 (-) 1140 WP_027907907.1 homoserine O-acetyltransferase -
  VXM67_RS03665 (VXM67_03665) - 802480..803070 (-) 591 WP_023378210.1 YggT family protein -
  VXM67_RS03670 (VXM67_03670) proC 803081..803899 (-) 819 WP_202675815.1 pyrroline-5-carboxylate reductase -
  VXM67_RS03675 (VXM67_03675) - 803938..804624 (-) 687 WP_023378212.1 YggS family pyridoxal phosphate-dependent enzyme -
  VXM67_RS03680 (VXM67_03680) pilT 804690..805688 (+) 999 WP_027907908.1 type IV pilus twitching motility protein PilT Machinery gene
  VXM67_RS03685 (VXM67_03685) - 805764..806390 (-) 627 WP_023378214.1 C40 family peptidase -
  VXM67_RS03690 (VXM67_03690) - 806559..806978 (+) 420 WP_023378215.1 TM2 domain-containing protein -
  VXM67_RS03695 (VXM67_03695) - 807020..807718 (-) 699 WP_023378216.1 SPOR domain-containing protein -
  VXM67_RS03700 (VXM67_03700) argS 807720..809456 (-) 1737 WP_023378217.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35697.30 Da        Isoelectric Point: 6.6988

>NTDB_id=927478 VXM67_RS03680 WP_027907908.1 804690..805688(+) (pilT) [Pseudomonas sp. Rh2]
MDVTDLLARAMAAGASDLHLAAGDVPLIRLDGELQRLDLPPLSATALHDGLAPLLAEAQCRQWAQGDELDLALDLPSLGR
IRLNLFRQSNGPAVAMRIIAQRIATLEALGLTEVFRAFAQHTDGLVLVGGPTGSGKSSTLAALVDQLNRERSLHIITLED
PIEVIHSCQRSLVNQREIGRHCRDFAQGLRSALRQDPDVIVIGELRDLETIRLALRAAETGHLVLATVHTRSAINSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVKRRGGGRVAAREVLVATPAVRNLIREGRAAQLQSVMQSGAAAGMKTMEGAM
EALRAEGLIDAL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=927478 VXM67_RS03680 WP_027907908.1 804690..805688(+) (pilT) [Pseudomonas sp. Rh2]
ATGGACGTGACTGACCTCTTGGCCCGGGCGATGGCTGCAGGGGCGAGTGACCTGCACCTGGCCGCGGGCGACGTACCGCT
GATTCGCCTGGATGGCGAGCTGCAACGCCTGGATCTGCCGCCGTTGTCGGCGACGGCGCTGCACGACGGGCTGGCGCCTT
TGCTGGCCGAAGCGCAGTGTCGCCAGTGGGCGCAAGGTGATGAGCTGGACCTGGCGCTCGATCTCCCCTCGCTTGGGCGT
ATCCGCTTGAATCTGTTTCGCCAGTCGAATGGGCCTGCGGTGGCCATGCGGATCATTGCGCAACGAATCGCCACACTCGA
AGCGTTAGGCCTGACAGAAGTGTTTCGAGCTTTTGCCCAACATACCGATGGCCTCGTCCTGGTAGGTGGGCCGACCGGTA
GCGGGAAGTCCAGCACGCTGGCCGCGCTGGTCGACCAGCTCAATCGCGAGCGCTCGCTGCACATCATCACATTGGAAGAC
CCTATCGAAGTTATCCACAGTTGCCAGCGCAGCCTGGTCAACCAGCGCGAGATCGGACGCCACTGTCGTGACTTCGCCCA
GGGGTTGCGCAGTGCACTGCGCCAAGACCCGGATGTCATCGTGATCGGCGAACTGAGGGATCTCGAAACGATTCGTCTGG
CGCTCCGGGCTGCCGAGACGGGGCATCTGGTGCTGGCGACCGTCCATACCCGGTCGGCGATCAACAGCATCGACCGTCTG
GTGGAGGTGTTTGCCGCCGAGGAGAAGCCGTTGGTCAGGGCCATGCTGGCCGAATCGCTGCGCTTGGTGGTAGCGCAGGT
GCTGGTGAAACGAAGAGGTGGCGGCCGGGTGGCGGCGCGGGAAGTGTTGGTGGCGACGCCCGCTGTGCGTAACCTGATCC
GAGAGGGGCGAGCGGCGCAGTTGCAGTCCGTGATGCAGTCGGGGGCGGCGGCGGGGATGAAGACGATGGAAGGAGCGATG
GAGGCGTTGAGGGCAGAAGGGCTGATCGACGCGTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.064

99.096

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.755

99.699

0.506
  pilT Vibrio cholerae strain A1552

50.755

99.699

0.506
  pilT Acinetobacter baylyi ADP1

50.606

99.398

0.503
  pilT Pseudomonas stutzeri DSM 10701

50.456

99.096

0.5
  pilT Acinetobacter baumannii strain A118

50.152

99.096

0.497
  pilT Acinetobacter baumannii D1279779

50.152

99.096

0.497
  pilT Acinetobacter nosocomialis M2

49.848

99.096

0.494
  pilT Neisseria meningitidis 8013

48.485

99.398

0.482
  pilT Legionella pneumophila strain Lp02

48.632

99.096

0.482
  pilT Legionella pneumophila strain ERS1305867

48.632

99.096

0.482
  pilT Neisseria gonorrhoeae MS11

48.182

99.398

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.061

99.398

0.458
  pilU Pseudomonas stutzeri DSM 10701

37.879

99.398

0.377
  pilU Vibrio cholerae strain A1552

37.346

97.59

0.364
  pilF Thermus thermophilus HB27

31.25

100

0.361