Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VIJ98_RS16410 Genome accession   NZ_CP142688
Coordinates   3539861..3540853 (-) Length   330 a.a.
NCBI ID   WP_323275034.1    Uniprot ID   -
Organism   Pantoea sp. S18     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3534861..3545853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VIJ98_RS16380 (VIJ98_16370) - 3535582..3536094 (+) 513 WP_154155290.1 SprT family zinc-dependent metalloprotease -
  VIJ98_RS16385 (VIJ98_16375) endA 3536208..3536915 (+) 708 WP_061720234.1 deoxyribonuclease I -
  VIJ98_RS16390 (VIJ98_16380) rsmE 3536990..3537721 (+) 732 WP_008107252.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VIJ98_RS16395 (VIJ98_16385) gshB 3537800..3538744 (+) 945 WP_323275032.1 glutathione synthase -
  VIJ98_RS16400 (VIJ98_16390) - 3538827..3539390 (+) 564 WP_008107249.1 YqgE/AlgH family protein -
  VIJ98_RS16405 (VIJ98_16395) ruvX 3539390..3539806 (+) 417 WP_008107247.1 Holliday junction resolvase RuvX -
  VIJ98_RS16410 (VIJ98_16400) pilT 3539861..3540853 (-) 993 WP_323275034.1 type IV pilus twitching motility protein PilT Machinery gene
  VIJ98_RS16415 (VIJ98_16405) - 3540870..3541577 (+) 708 WP_008107243.1 YggS family pyridoxal phosphate-dependent enzyme -
  VIJ98_RS16420 (VIJ98_16410) - 3541590..3542144 (+) 555 WP_008107240.1 YggT family protein -
  VIJ98_RS16425 (VIJ98_16415) - 3542165..3542758 (+) 594 WP_154155286.1 XTP/dITP diphosphatase -
  VIJ98_RS16430 (VIJ98_16420) hemW 3542751..3543902 (+) 1152 WP_323275036.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35861.28 Da        Isoelectric Point: 6.6651

>NTDB_id=926446 VIJ98_RS16410 WP_323275034.1 3539861..3540853(-) (pilT) [Pantoea sp. S18]
MELDEIVALSVKHNAADLHLCSGHLPHWRRQGVLEPIPQQNALDGGWLEAFMQQWLTPPQQAELEENGHVDFAITLASGI
RLRANLFMQRHGLSLALRLIASHAPDLSSLHLPDVVSQLLQLEEGLILITGATGSGKSTTLAAMVDCLNRQQARHILTLE
DPIEFVHRSQRSLIQQREVGAHCASFQHGLKAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGATQAVDR
LVDVFPAEEKNLVRTQLAGSLKAVLAQRLVPAKAGGRIGLFEVLVATPAVANLIREGKMHQLPGVLQTGAQAGMQTFAQS
EQARQLAGMI

Nucleotide


Download         Length: 993 bp        

>NTDB_id=926446 VIJ98_RS16410 WP_323275034.1 3539861..3540853(-) (pilT) [Pantoea sp. S18]
ATGGAGTTGGATGAAATTGTGGCCCTTAGTGTAAAGCATAACGCCGCCGATCTGCACCTTTGCAGCGGACATTTGCCGCA
CTGGCGGCGACAAGGCGTGCTGGAGCCGATTCCGCAGCAAAACGCACTGGATGGCGGCTGGCTGGAAGCCTTTATGCAGC
AGTGGCTGACGCCACCGCAACAGGCCGAGCTGGAGGAGAACGGCCACGTTGACTTTGCCATCACGCTTGCCAGCGGCATT
CGACTGCGCGCCAACCTTTTTATGCAGCGTCACGGCTTATCGCTGGCGCTGCGGTTGATTGCCAGCCACGCGCCCGATCT
CAGCAGCCTGCATTTACCCGACGTAGTGAGCCAGTTGTTACAGCTGGAAGAGGGGCTGATTTTAATTACCGGTGCCACCG
GCAGCGGCAAATCAACTACGCTGGCGGCGATGGTGGATTGCCTGAACCGCCAACAGGCGCGGCACATCCTGACGCTGGAA
GATCCGATTGAGTTTGTGCATCGCAGCCAGCGATCGCTGATTCAGCAGCGCGAAGTCGGCGCACATTGCGCATCGTTTCA
ACACGGGCTGAAAGCTGCGCTGCGCGAAGATCCTGATGTGATTCTGCTCGGTGAATTGCGTGATAGCGAAACCATCCGCC
TGGCGCTCACTGCGGCAGAAACCGGCCATCTGGTGCTGGCTACGCTCCATACGCGCGGCGCAACCCAGGCGGTTGATCGG
CTGGTGGATGTGTTTCCCGCTGAGGAGAAAAATCTGGTGCGCACCCAATTAGCGGGCAGCCTGAAAGCGGTGCTGGCGCA
GCGTTTAGTGCCGGCGAAAGCGGGCGGGCGTATTGGCTTGTTTGAAGTGTTGGTAGCGACGCCAGCGGTGGCGAATCTGA
TTCGCGAGGGCAAGATGCATCAGTTGCCTGGCGTGTTGCAAACCGGTGCGCAGGCGGGCATGCAGACATTTGCGCAGAGC
GAGCAGGCGCGGCAGTTAGCGGGGATGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.048

100

0.485
  pilT Neisseria gonorrhoeae MS11

48.048

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.78

99.394

0.485
  pilT Vibrio cholerae strain A1552

48.78

99.394

0.485
  pilT Pseudomonas stutzeri DSM 10701

47.319

96.061

0.455
  pilT Acinetobacter baylyi ADP1

46.875

96.97

0.455
  pilT Legionella pneumophila strain ERS1305867

46.708

96.667

0.452
  pilT Legionella pneumophila strain Lp02

46.708

96.667

0.452
  pilT Acinetobacter nosocomialis M2

46.25

96.97

0.448
  pilT Acinetobacter baumannii D1279779

46.25

96.97

0.448
  pilT Acinetobacter baumannii strain A118

46.25

96.97

0.448
  pilT Pseudomonas aeruginosa PAK

46.372

96.061

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.41

97.576

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.925

97.576

0.409
  pilU Vibrio cholerae strain A1552

41.121

97.273

0.4
  pilU Acinetobacter baylyi ADP1

37.578

97.576

0.367