Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AIN32_RS03955 Genome accession   NZ_CP140737
Coordinates   823348..824328 (+) Length   326 a.a.
NCBI ID   WP_001055642.1    Uniprot ID   A0A3V9WZJ6
Organism   Salmonella enterica subsp. enterica serovar Newport strain CFSAN003353     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 818348..829328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AIN32_RS03925 (AIN32_003925) - 818926..819933 (+) 1008 WP_000252193.1 DUF1202 domain-containing protein -
  AIN32_RS03930 (AIN32_003930) hemW 820024..821160 (-) 1137 WP_001096528.1 radical SAM family heme chaperone HemW -
  AIN32_RS03935 (AIN32_003935) - 821153..821746 (-) 594 WP_001174764.1 XTP/dITP diphosphatase -
  AIN32_RS03940 (AIN32_003940) yggU 821754..822044 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  AIN32_RS03945 (AIN32_003945) - 822041..822607 (-) 567 WP_001094848.1 YggT family protein -
  AIN32_RS03950 (AIN32_003950) - 822626..823330 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  AIN32_RS03955 (AIN32_003955) pilT 823348..824328 (+) 981 WP_001055642.1 type IV pilus twitching motility protein PilT Machinery gene
  AIN32_RS03960 (AIN32_003960) - 824460..825146 (+) 687 WP_000098325.1 IclR family transcriptional regulator domain-containing protein -
  AIN32_RS03965 (AIN32_003965) ruvX 825193..825609 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  AIN32_RS03970 (AIN32_003970) - 825609..826172 (-) 564 WP_001053171.1 YqgE/AlgH family protein -
  AIN32_RS03975 (AIN32_003975) gshB 826388..827335 (-) 948 WP_000593248.1 glutathione synthase -
  AIN32_RS03980 (AIN32_003980) rsmE 827355..828086 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AIN32_RS03985 (AIN32_003985) endA 828163..828870 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36125.28 Da        Isoelectric Point: 7.5355

>NTDB_id=915238 AIN32_RS03955 WP_001055642.1 823348..824328(+) (pilT) [Salmonella enterica subsp. enterica serovar Newport strain CFSAN003353]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=915238 AIN32_RS03955 WP_001055642.1 823348..824328(+) (pilT) [Salmonella enterica subsp. enterica serovar Newport strain CFSAN003353]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3V9WZJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.254

100

0.368
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365