Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SFB89_RS04055 Genome accession   NZ_CP140614
Coordinates   808007..809065 (-) Length   352 a.a.
NCBI ID   WP_331775666.1    Uniprot ID   -
Organism   Sulfurospirillum sp. 1612     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 803007..814065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SFB89_RS04030 (SFB89_04030) - 803180..804427 (-) 1248 WP_331775661.1 serine hydroxymethyltransferase -
  SFB89_RS04035 (SFB89_04035) lysS 804431..805954 (-) 1524 WP_331775662.1 lysine--tRNA ligase -
  SFB89_RS04040 (SFB89_04040) - 805968..806444 (-) 477 WP_331775663.1 Fur family transcriptional regulator -
  SFB89_RS04045 (SFB89_04045) - 806452..806991 (-) 540 WP_331775664.1 CvpA family protein -
  SFB89_RS04050 (SFB89_04050) - 806995..807939 (-) 945 WP_331775665.1 GGDEF domain-containing protein -
  SFB89_RS04055 (SFB89_04055) pilT 808007..809065 (-) 1059 WP_331775666.1 type IV pilus twitching motility protein PilT Machinery gene
  SFB89_RS04060 (SFB89_04060) gatC 809076..809366 (-) 291 WP_331775667.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  SFB89_RS04065 (SFB89_04065) - 809485..810069 (+) 585 WP_331775668.1 hypothetical protein -
  SFB89_RS04070 (SFB89_04070) - 810066..811076 (+) 1011 WP_331775669.1 PQQ-binding-like beta-propeller repeat protein -
  SFB89_RS04075 (SFB89_04075) - 811079..811420 (+) 342 WP_331775670.1 hypothetical protein -
  SFB89_RS04080 (SFB89_04080) - 811407..812027 (+) 621 WP_331775671.1 type III pantothenate kinase -
  SFB89_RS04085 (SFB89_04085) hisG 812021..812632 (+) 612 WP_331775672.1 ATP phosphoribosyltransferase -
  SFB89_RS04090 (SFB89_04090) - 812647..813027 (-) 381 WP_331775673.1 response regulator -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39410.34 Da        Isoelectric Point: 6.7778

>NTDB_id=914879 SFB89_RS04055 WP_331775666.1 808007..809065(-) (pilT) [Sulfurospirillum sp. 1612]
MNIKSLLKNVLNYKASDLHIVSRSEPQIRIDGVLVPLDLEKLSGVAIEEMCYTLLSDKQKKALEEEKELDFAIEFPDIGR
FRANYYYTMNGELAAAFRIIPIEIPSLDELRTPPIFKDLVQREKGLILVTGPTGSGKSTTLAALLNEINLTENKHIITIE
DPVEFVHQNKKSLFSHRNVGTDTKSFARGLKFSLREDPDVILVGEIRDQETISTAITAAETGHLVFATLHTNSAVQTINR
IVDSFEGAEQIQVRNMLSTSLYAVISQSLLPKVGSGRIAIHEILINNAAISNLIRENKLHQIYSQMQLNQQKTGMMTQTQ
SLLKHLNAKLITKENAIRYSTQTQELMNSLGI

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=914879 SFB89_RS04055 WP_331775666.1 808007..809065(-) (pilT) [Sulfurospirillum sp. 1612]
ATGAACATCAAATCATTACTCAAAAACGTTCTCAATTATAAGGCTTCTGATCTTCATATTGTCAGTCGTAGTGAACCTCA
GATTCGGATTGATGGGGTATTAGTACCTCTAGATCTTGAAAAACTCTCAGGAGTTGCAATAGAAGAGATGTGTTACACTC
TGCTCAGTGACAAACAAAAAAAGGCTCTTGAAGAGGAAAAAGAACTCGATTTTGCGATAGAATTCCCAGATATCGGACGT
TTTCGTGCCAACTACTATTACACTATGAATGGTGAATTAGCCGCTGCTTTTAGGATTATTCCTATTGAAATTCCTTCCTT
AGATGAACTTAGAACTCCCCCTATTTTCAAGGATTTAGTCCAAAGAGAAAAAGGGTTAATCTTAGTCACCGGACCAACAG
GAAGTGGGAAATCAACCACGCTTGCGGCATTACTAAATGAAATTAATCTCACAGAGAACAAGCACATTATCACGATTGAA
GATCCTGTCGAATTTGTGCATCAAAACAAAAAATCTCTCTTTTCTCATAGAAATGTCGGAACCGATACTAAAAGTTTTGC
GAGAGGATTGAAATTTTCACTGCGTGAAGATCCCGATGTCATTCTCGTGGGTGAAATTCGAGATCAAGAAACAATCAGTA
CGGCCATCACAGCCGCTGAAACCGGACACCTTGTTTTTGCAACCTTGCATACAAACTCAGCTGTCCAAACGATTAATAGA
ATTGTCGATAGTTTTGAAGGTGCGGAACAAATTCAAGTCAGAAATATGCTCTCAACTTCTCTTTATGCCGTCATTTCACA
AAGCTTACTTCCTAAAGTCGGTAGCGGAAGAATTGCGATTCATGAGATACTCATCAATAATGCAGCGATTTCGAATCTTA
TTAGAGAAAATAAATTGCATCAAATATACTCGCAAATGCAGTTAAATCAACAAAAAACCGGTATGATGACACAAACACAA
TCCCTTCTCAAACATTTAAATGCTAAATTAATAACAAAAGAGAATGCGATTCGCTACTCCACACAAACACAAGAGTTGAT
GAACAGCTTAGGAATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

50.72

98.58

0.5
  pilT Neisseria meningitidis 8013

50.432

98.58

0.497
  pilT Legionella pneumophila strain Lp02

51.488

95.455

0.491
  pilT Legionella pneumophila strain ERS1305867

51.488

95.455

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.275

98.011

0.483
  pilT Acinetobacter baylyi ADP1

49.275

98.011

0.483
  pilT Acinetobacter baumannii D1279779

48.696

98.011

0.477
  pilT Acinetobacter baumannii strain A118

48.696

98.011

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.025

91.193

0.474
  pilT Vibrio cholerae strain A1552

52.025

91.193

0.474
  pilT Acinetobacter nosocomialis M2

47.839

98.58

0.472
  pilT Pseudomonas aeruginosa PAK

49.107

95.455

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.363

98.58

0.418
  pilU Acinetobacter baylyi ADP1

38.889

97.159

0.378
  pilU Vibrio cholerae strain A1552

38.218

98.864

0.378
  pilU Pseudomonas stutzeri DSM 10701

37.644

98.864

0.372